Employees in science and technology enterprises was taken as the survey object, and dual work passion was taken as the intermediary variable to further explore the impact mechanism of career call on turnover intention. Based on the correlation analysis and regression analysis of 404 employee sample data collected by high-tech enterprises, the empirical study shows that career calling has a significant positive impact on work passion, but no significant impact on turnover intention. Forced work passion has a significant positive impact on turnover intention, while harmonious work passion has a significant negative impact on turnover intention. Work passion plays a completely intermediary role in career calling and turnover intention. Based on the research conclusions, it can be concluded that improving the occupational calling level of employees in science and technology enterprises can enhance the work passion of employees, reduce the turnover tendency and improve work efficiency.