Home Journals Articles Updates About
CN
image
收藏切换
Functions of ClyA_Cry6Aa-like pore-forming toxin proteins in Sclerotinia nivalis
收藏切换
PDF
Yanxia CAO1, Shishuai GENG2, Hengjia ZHANG1, Wenjie LI1, Jian LIU2, Shuhan YANG3, Sijun WANG2, Sanhong FAN2, *, Xiaoping HU1, *
Mycosystema | 2026, 45(4) : 250263
Less
收藏切换
Mycosystema | 2026, 45(4): 250263
• Research paper •
Functions of ClyA_Cry6Aa-like pore-forming toxin proteins in Sclerotinia nivalis
Full
Yanxia CAO1, Shishuai GENG2, Hengjia ZHANG1, Wenjie LI1, Jian LIU2, Shuhan YANG3, Sijun WANG2, Sanhong FAN2, *, Xiaoping HU1, *
Affiliations
  • 1 Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China
  • 2 College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China
  • 3 College of Chemistry and Pharmacy, Northwest A&F University, Yangling 712100, Shaanxi, China
Published: 2026-04-22 doi: 10.13346/j.mycosystema.250263
Outline
收藏切换

Pore-forming toxins (PFTs) are present in fungi, but their functions remain poorly understood. In this study, analysis of differentially expressed genes between sclerotia and mycelia of Sclerotinia nivalis revealed that genes encoding pore-forming toxin proteins were highly expressed in sclerotia. Further proteomic profiling by mass spectrometry confirmed the abundant accumulation of these proteins in sclerotia. Sequence and structural analyses indicated that three pore-forming toxin proteins SnCry6Aa1, SnCry6Aa2, and SnCry6Aa3 were encoded by S. nivali SnTB1. All of which contain the conserved ClyA_Cry6Aa-like PFTs domain and exhibit the characteristic three-dimensional structure of α-PFTs. The recombinant SnCry6Aa1 and SnCry6Aa2 were successfully expressed and purified using a prokaryotic system, and their hemolytic activity and nematidal/insecticidal toxicity were subsequently assessed. The results showed that SnCry6Aa1 exhibited significant hemolytic activity at concentrations over 6.25 ng/mL under acidic conditions, whereas no similar activity was detected for SnCry6Aa2. Neither SnCry6Aa1 nor SnCry6Aa2 showed notable toxicity against Caenorhabditis elegans, Helicoverpa armigera, Spodoptera litura, Grapholita molesta, or Bradysia odoriphaga. However, both displayed low but detectable insecticidal activity against larvae of Pnyxia scabiei, with corrected mortality rates of 14% and 17%, respectively. This study is the first to identify the presence of ClyA_Cry6Aa-like PFTs at high abundance in the sclerotia of S. nivalis and provides initial insights into their biological roles, laying a foundation for further functional studies and potential applications of these proteins.

Sclerotinia nivalis  /  sclerotium  /  ClyA_Cry6Aa-like pore-forming toxin protein  /  erythrocyte lysis  /  insecticidal efficiency
Yanxia CAO, Shishuai GENG, Hengjia ZHANG, Wenjie LI, Jian LIU, Shuhan YANG, Sijun WANG, Sanhong FAN, Xiaoping HU. Functions of ClyA_Cry6Aa-like pore-forming toxin proteins in Sclerotinia nivalis[J]. Mycosystema, 2026 , 45 (4) : 250263 - . DOI: 10.13346/j.mycosystema.250263
Funding
Less
  • China Agriculture Research System(CARS-03-37)
  • Innovation Team Project for Crop Disease and Pest Monitoring and Management of Northwest A&F University(XYTD2023-04)
Appendix
Less
Year 2026 volume 45 Issue 4
PDF
117
51
Cite this Article
BibTeX
Article Info
doi: 10.13346/j.mycosystema.250263
  • Receive Date：2025-09-02
  • Online Date：2026-04-30
  • Published：2026-04-22
Article Data
Affiliations
History
  • Received：2025-09-02
  • Accepted：2025-09-30
Funding
China Agriculture Research System(CARS-03-37)
Innovation Team Project for Crop Disease and Pest Monitoring and Management of Northwest A&F University(XYTD2023-04)
Affiliations
    1 Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China
    2 College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China
    3 College of Chemistry and Pharmacy, Northwest A&F University, Yangling 712100, Shaanxi, China

Corresponding:

*HU Xiaoping, ;
FAN Sanhong,
References
Share
https://castjournals.cast.org.cn/joweb/jwxb/EN/10.13346/j.mycosystema.250263
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House