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Screening of molecular markers specific for Tilletia controversa based on the whole genome sequence
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Ruifang GAO1, *, Peng ZHAO2, Yijing TANG1, Xiaoxiao LIU1, Xinyu XU1, Mingsen ZHENG1
Mycosystema | 2026, 45(2) : 250175
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Mycosystema | 2026, 45(2): 250175
• Research paper •
Screening of molecular markers specific for Tilletia controversa based on the whole genome sequence
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Ruifang GAO1, *, Peng ZHAO2, Yijing TANG1, Xiaoxiao LIU1, Xinyu XU1, Mingsen ZHENG1
Affiliations
  • 1 Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Customs District of P. R. China, Shenzhen 518045, Guangdong, China
  • 2 Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Published: 2026-02-22 doi: 10.13346/j.mycosystema.250175
Outline
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Wheat dwarf smut, caused by Tilletia controversa (TCK), is a dangerous fungal wheat disease that is highly destructive and difficult to eradication, possessing a significant impact. The disease is known as “No. 1 disease of wheat”, and is listed as a quarantine pest by European Plant Protection Organisation (EPPO), Australia, China and other countries/regions and organizations. Due to difficulties for distinguishing TCK from other closely related species such as Tilletia laevis in levels of morphological characteristics and molecular biology, misdiagnosis easily happen in quarantine inspections. In this study, TCK-specific molecular markers were developed at the genomic level, and four candidate genes, namely OG0008430, OG0008601, OG0008603 and OG0008610, were screened. Eight pairs of primers and four probes based on these four candidate genes were designed. Validation of combinations among the primers and probes were verified, and two sets of “triple + single” real-time fluorescence PCR methods were established. This combined method can effectively distinguish target species (TCK) from closely related species, achieving accurate identification of TCK, and it can also be used to distinguish different species within the genus Tilletia.

Tilletia controversa  /  multiple real-time fluorescence PCR  /  molecular marker  /  genome  /  detection method
Ruifang GAO, Peng ZHAO, Yijing TANG, Xiaoxiao LIU, Xinyu XU, Mingsen ZHENG. Screening of molecular markers specific for Tilletia controversa based on the whole genome sequence[J]. Mycosystema, 2026 , 45 (2) : 250175 - . DOI: 10.13346/j.mycosystema.250175
Funding
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  • Scientific Research Projects of the General Administration of Customs(2023HK001)
  • Key Research and Development Projects of the Xizang Autonomous Region Science and Technology Plan(XZ202201ZY0011N)
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Year 2026 volume 45 Issue 2
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Article Info
doi: 10.13346/j.mycosystema.250175
  • Receive Date：2025-06-10
  • Online Date：2026-04-29
  • Published：2026-02-22
Article Data
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History
  • Received：2025-06-10
  • Accepted：2025-07-31
Funding
Scientific Research Projects of the General Administration of Customs(2023HK001)
Key Research and Development Projects of the Xizang Autonomous Region Science and Technology Plan(XZ202201ZY0011N)
Affiliations
    1 Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Customs District of P. R. China, Shenzhen 518045, Guangdong, China
    2 Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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