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ORCID: SHI Minmin (0009-0001-1197-5941)
Outline
Coprinellus micaceus, the most common species of coprinoid fungi with extremely abundant resources, having potential in enhancing seed germination of medicinal orchid Cremastra appendiculata was investigated. Fruiting bodies of Co. micaceus were collected from the wild, and the hyphae were isolated from inner side of hollow stipe. Hyphal tip purification method was used to obtain multiple strains, and the two strains first obtained were named as S-1 and S-2 for subsequent experiments. S-1 was co-cultivated with Cr. appendiculata seeds on oat media to observe seed germination. Acid fuchsin staining was used to observe the morphology of protocorms formed on oat media and the hyphal colonization, and ITS sequencing of S-1 was performed. Spawn of S-2 was fragmented and mixed into potting soil, and covered with soil to induce fruiting bodies, while seeds of Cr. appendiculata were sown to simulate field inoculation. Endogenous strain were isolated from the protocorms, yielding S-3, and ITS sequencing of S-3 was performed. Additionally, dried and ground mycelial fragments of Co. micaceus were added to oat media to test whether the fungus could still promote seed germination under sterile conditions. The results showed that the fruiting body morphology and ITS sequences of both S-1 and S-3 matched those of Co. micaceus. Co. micaceus enhanced seed germination in both oat media and soil substrates. Acid fuchsin staining revealed that protocorms could digest invasive hyphae. Moreover, the mycelial powder of Co. micaceus in sterile oat media could also promote the germination of Cr. appendiculata seeds. This study demonstrates that Co. micaceus possesses potential to support the conservation and cultivation of Cr. appendiculata.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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