To establish a quality control method for Aquilaria sinensis and provide a basis for improving its quality standards.
A fingerprint identification method for Aquilaria sinensis was established. The contents of 4-phenyl-2-butanone, 4-methoxyphenyl-2-butanone, and Nootkatone in Aquilaria sinensis were determined by GC-MS.
Reference fingerprint for Aquilaria sinensis was established. The similarity among 25 batches of samples ranged from 0.718 to 0.994. In the content determination, 4-phenyl-2-butanone, 4-methoxyphenyl-2-butanone, and Nootkatone showed good linear relationships within the concentration ranges of 0.14-11.58, 0.27-21.56, 0.65-52.14 µg·mL-1, respectively.The average recovery rates of the three components were 92.5% (n=9), 92.1% (n=9), and 90.2% (n=9).
The fingerprint chromatogram and the content determination methods of the three components established in this studyt can be used for multi-index quality evaluation of Aquilaria sinensis.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |