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Study on Identification and Content Determination in Material Benchmark of Huangqi Guizhi Wuwutang
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Ming CHEN, Liubing LI, Jiahui ZHAN, Qifu LU
Pharmacy Today | 2026, 36(3) : 179 - 184
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Pharmacy Today | 2026, 36(3): 179-184
Study on Identification and Content Determination in Material Benchmark of Huangqi Guizhi Wuwutang
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Ming CHEN, Liubing LI, Jiahui ZHAN, Qifu LU
Affiliations
  • Guangzhou BaiYunShan PanGaoShou Pharmaceutical Co., Ltd., Guangzhou, Guangdong 511490, China
Published: 2026-03-25 doi: 10.12048/j.issn.1674-229X.2026.03.004
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OBJECTIVE

To establish a TLC identification method and the content determination of paeoniflorin in material benchmark of Huangqi Guizhi Wuwutang.

METHODS

Thin-layer chromatography was employed for the identification of Astragali Radix (Astragalus root), Cinnamomi Ramulus (cinnamon twig), Paeoniae Radix Alba (white peony root) and Zingiberis Rhizoma Recens (fresh ginger) in material benchmark of Huangqi Guizhi Wuwutang. The quantitative analysis of paeoniflorin was performed by high performance liquid chromatography (HPLC) on a Thermo Hypersil GOLD C18 column (4.6 mm × 250 mm, 5 μm). The mobile phase was acetonitrile-0.1% phosphoric acid solution (14:86) with isocratic elution; the flow rate was 1.0 mL·min-1, the detection wavelength was 230 nm, and the column temperature was 25 ℃.

RESULTS

The linear range of paeoniflorin was 148.00-2 368.00 μg·mL-1 (r = 0.999 9). The average recovery of paeoniflorin was 98.48% (RSD = 0.95%).

CONCLUSION

The established method is sensitive, simple, reliable and suitable for the quality control in the research and development of the classic formula Huangqi Guizhi Wuwutang.

Huangqi Guizhi Wuwutang  /  thin-layer chromatography (TLC)  /  paeoniflorin  /  content determination  /  quality control
陈明, 黎柳冰, 詹家辉, 卢其福. 经典名方黄芪桂枝五物汤物质基准的薄层色谱鉴别与含量测定. 今日药学, 2026 , 36 (3) : 179 -184 . DOI: 10.12048/j.issn.1674-229X.2026.03.004
Ming CHEN, Liubing LI, Jiahui ZHAN, Qifu LU. Study on Identification and Content Determination in Material Benchmark of Huangqi Guizhi Wuwutang[J]. Pharmacy Today, 2026 , 36 (3) : 179 -184 . DOI: 10.12048/j.issn.1674-229X.2026.03.004
Appendix
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Year 2026 volume 36 Issue 3
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Article Info
doi: 10.12048/j.issn.1674-229X.2026.03.004
  • Receive Date：2025-06-03
  • Online Date：2026-09-23
  • Published：2026-03-25
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  • Received：2025-06-03
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    Guangzhou BaiYunShan PanGaoShou Pharmaceutical Co., Ltd., Guangzhou, Guangdong 511490, China
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https://castjournals.cast.org.cn/joweb/jryx/EN/10.12048/j.issn.1674-229X.2026.03.004
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Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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