Full
Outline
To assess uncertainty in the determination of vortioxetine concentrations in serum from patients with psychiatric disorders using HPLC-MS/MS methodology.
For the determination of vortioxetine concentration in human serum, the uncertainty introduced by repeated measurements was evaluated using the type A evaluation. The uncertainties contributed from standard weighing, purity, solution preparation, preparation of drug-containing serum, extraction recovery, matrix effect, calibration curve fitting and instrumental precision were assessed using the type B evaluation. Subsequently, the synthetic and extended uncertainties were calculated.
The extended uncertainties for low (0.6 ng·mL-1), medium (60 ng·mL-1), and high (120 ng·mL-1) concentrations of vortioxetine in human serum were 0.67, 4.10, and 10.49 ng·mL-1, respectively, when confidence probability was P = 95% (k = 2).
The method employed in this study exhibits high accuracy and is suitable for the therapeutic drug monitoring of vortioxetine. The primary source of uncertainty arises from the calibration curve fitting, with particularly elevated uncertainty values introduced at the low concentration (0.6 ng·mL -1). Implementing a rational linear range and optimizing calibration curve fitting can enhance the accuracy of measurement results.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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