To improve the allergic reaction test method of Cattle Encephalon Glycoside and Ignotin Injection by using the direct popliteal lymph node (D-PLNA) and reporter antigen popliteal lymph node (RA-PLNA) models in mice.

In the D-PLNA model, the Cattle Encephalon Glycoside and Ignotin Injection group, normal saline control group and positive drug D-penicillamine hydrochloride control group were set up. In the RA-PLNA model, the above groups were respectively added with reporter antigens TNP-Ficoll and TNP-OVA, and 50 μL of the drug was administered to the left toe pad. On the 7th day, the mice were sacrificed, and the popliteal lymph nodes were isolated, ground to prepare lymphocyte single-cell suspensions and counted to calculate the cell index. The cell suspension was cultured in 96-well plates, and after co-incubation with stimulators, the contents of IgE and MCP-1 in the cell supernatant were determined by ELISA.

Compared with the normal saline control group, in the RA-PLNA model of mice, the number of cells in the popliteal lymph nodes on the drug administration side increased in the group added with TNP-OVA (P < 0.05), and the contents of IgE and MCP-1 both increased (P < 0.05).

Cattle encephalon glycoside and ignotin injection only caused a positive lymph node reaction in mice when TNP-OVA was added, indicating that there may be small molecule substances in Cattle Encephalon Glycoside and Ignotin Injection that cooperate with TNP-OVA to induce allergic reactions.