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Outline
To establish a method for the simultaneous determination of catechin, epicatechin, isoquercitrin, quercetin, madecassic acid, asiatic acid, corosolic acid, oleanolic acid, ursolic acid, β-sitosterol, ethanol-soluble extractive, total ash and acid-insoluble ash in Actinidiae chinensis Radix (Radix Actinidiae Chinensis, Tengligen), and explore the application of chemometrics and weighted TOPSIS in the quality evaluation of Actinidiae chinensis radix.
An HPLC method was adopted with acetonitrile-0.2% phosphoric acid solution as the mobile phase under gradient elution.The detection wavelengths were set at 280 nm and 210 nm. Chemometric analysis (principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA)) was performed on the multi-index content results, and a weighted TOPSIS model was established to comprehensively evaluate the overall quality of Actinidiae chinensis radix.
The methodological validation results of the external standard method for HPLC simultaneous determination were consistent with the requirements of the Chinese Pharmacopoeia (ChP). The results of chemometrics analysis showed that 18 batches of Actinidiae chinensis radix were clustered into three categories, and epicatechin, β-sitosterol, asiatic acid, ursolic acid, quercetin and catechin were the main potential quality markers affecting the quality of Actinidiae chinensis radix.The Euclidean closeness degree (Ci) of the weighted TOPSIS method ranged from 0.143 8 to 0.763 4, indicating a certain degree of batch-to-batch variation in Actinidiae chinensis radix.
The multi-index quantitative evaluation method combined with chemometrics and weighted TOPSIS method of Actinidiae chinensis radix is efficient and accurate, with good repeatability and stability, which can be used for the quality evaluation of Actinidiae chinensis radix.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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