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A Method for Determining the Isoelectric Point of Human Basic Fibroblast Growth Factor
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Junfang SHI1, 2, Yachao TAN2, Xiao FENG2, Wei XUE1, *
Pharmacy Today | 2026, 36(1) : 12 - 15
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Pharmacy Today | 2026, 36(1): 12-15
A Method for Determining the Isoelectric Point of Human Basic Fibroblast Growth Factor
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Junfang SHI1, 2, Yachao TAN2, Xiao FENG2, Wei XUE1, *
Affiliations
  • 1.Jinan University School of Biomedical Engineering, Guangzhou, Guangdong 510632, China
  • 2.Langtai Biopharmaceutical Co., Ltd., Foshan, Guangdong 528000, China
Published: 2026-01-25 doi: 10.12048/j.issn.1674-229X.2026.01.003
Outline
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OBJECTIVE

To establish an isoelectric focusing (IEF) analysis method for the physicochemical testing of human basic fibroblast growth factor (hbFGF).

METHODS

By comparing different IEF test conditions, the optimal method for determining the isoelectric point of hbFGF protein was screened, and the specificity, precision and durability of this method were methodologically verified.

RESULTS

The optimal system was the ampholyte system of Bio-Lyte and Ampholyte (2∶1). PBS buffer showed no interference with this method. The average pI value for repeatability was 10.0, with an RSD of 0.82% (≤3.0%). The average pI value for intermediate precision was 10.0, with a total RSD of 0.98% (≤6.0%). In terms of durability under different voltages (460, 480 and 500 V), the average pI value was 10.0, with an RSD of 1.3% (≤3.0%). In terms of durability under different electrophoresis times (70 min, 90 min, and 110 min), the average pI value was 10.0, with an RSD of 2.0% (≤3.0%).

CONCLUSION

The IEF method established for detecting the isoelectric point of hbFGF protein is accurate, durable and highly specific. It has high stability and can be used for the routine daily isoelectric point detection of hbFGF protein.

human basic fibroblast growth factor (hbFGF)  /  isoelectric focusing electrophoresis (IEF)  /  methodological validation
石俊芳, 谭雅超, 冯笑, 薛巍. 一种用于人碱性成纤维细胞生长因子等电点测定的方法. 今日药学, 2026 , 36 (1) : 12 -15 . DOI: 10.12048/j.issn.1674-229X.2026.01.003
Junfang SHI, Yachao TAN, Xiao FENG, Wei XUE. A Method for Determining the Isoelectric Point of Human Basic Fibroblast Growth Factor[J]. Pharmacy Today, 2026 , 36 (1) : 12 -15 . DOI: 10.12048/j.issn.1674-229X.2026.01.003
Appendix
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Year 2026 volume 36 Issue 1
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Article Info
doi: 10.12048/j.issn.1674-229X.2026.01.003
  • Receive Date：2025-10-29
  • Online Date：2026-09-23
  • Published：2026-01-25
Article Data
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History
  • Received：2025-10-29
Funding
Affiliations
    1.Jinan University School of Biomedical Engineering, Guangzhou, Guangdong 510632, China
    2.Langtai Biopharmaceutical Co., Ltd., Foshan, Guangdong 528000, China
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https://castjournals.cast.org.cn/joweb/jryx/EN/10.12048/j.issn.1674-229X.2026.01.003
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表12种不同金属材料的力学参数
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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