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Visualizing enzyme-induced mineralization in fractures
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Yang Xiaoa, Chang Zhaoa, *, Qingyun Fanga, Xiang Heb, Jian Chub, Hanlong Liua
Journal of Rock Mechanics and Geotechnical Engineering | 2026, 18(5) : 4023 - 4033
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Journal of Rock Mechanics and Geotechnical Engineering | 2026, 18(5): 4023-4033
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Visualizing enzyme-induced mineralization in fractures
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Yang Xiaoa, Chang Zhaoa, *, Qingyun Fanga, Xiang Heb, Jian Chub, Hanlong Liua
Affiliations
  • aKey Laboratory of New Technology for Construction of Cities in Mountain Area, Chongqing University, Chongqing, 400045, China
  • bSchool of Civil and Environmental Engineering, Nanyang Technological University, 639798, Singapore
  • Dr. Yang Xiao was the "China Highly Cited Scholar" for a seventh consecutive year and was awarded with one national second prize, two first awards of ministerial or provincial science and technology. He is also a three-time recipient of the Fredlund Award from the Canadian Geotechnical Society. He has published more than 170 papers in reputable journals. Dr. Xiao was the associate editor of International Journal of Geomechanics (ASCE), and Canadian Geotechnical Journal, etc., and the executive deputy Editor-in-Chief of Biogeotechnics. The research group headed by Dr. Xiao has made some achievements in the fields of bacterial-scale mechanism of bio-mineralization, grain-scale and element-scale mechanisms of bio-treated soils, constitutive theory of bio-cemented soils, and field-scale bio-treatment method.

Published: 2026-05-25 doi: 10.1016/j.jrmge.2025.06.035
Outline
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Effective sealing of geological fractures is essential for subsurface stability and mitigating environmental risks such as groundwater contamination and inefficient CO2 sequestration. Enzymatically Induced Carbonate Precipitation (EICP) offers a promising bio-mediated approach due to its ability to fill and seal fractures. However, real-time precipitation patterns and clogging behavior of EICP under varying fracture and flow conditions remain poorly understood. This study employs a transparent fracture model with visualization to systematically investigate the effects of fracture aperture, flow conditions, and surface roughness on EICP-mediated sealing. Results indicate that fractures with narrower apertures promote tortuous finger-like flow paths, while wider-aperture fractures show more uniform deposition, with fewer but wider preferential flow paths. An appropriate injection rate around 1 mL/min ensures uniform precipitation and effective clogging, avoiding inlet clogging at lower rates (0.1 mL/min) and flushing effect reducing deposition at higher rates (10 mL/min). Additionally, rough fractures exhibit higher precipitation efficiency and greater permeability reduction, driven by their irregular surface geometry, which creates more deposition sites and complex flow compared to smooth fractures. Image processing reveals that precipitation patterns in rough fractures match closely with aperture distribution, compared to more concentrated deposition in smooth fractures. These findings provide insights for optimizing EICP-mediated fracture sealing, with implications for groundwater protection and geotechnical practices.

Enzymatically Induced Carbonate  /  Precipitation (EICP)  /  Fracture healing  /  Fracture roughness  /  Fracture aperture  /  Visualization method  /  Permeability evolution
Yang Xiao, Chang Zhao, Qingyun Fang, Xiang He, Jian Chu, Hanlong Liu. Visualizing enzyme-induced mineralization in fractures[J]. Journal of Rock Mechanics and Geotechnical Engineering, 2026 , 18 (5) : 4023 -4033 . DOI: 10.1016/j.jrmge.2025.06.035
  • National Natural Science Foundation of China(52425806; 52378325)
  • Fundamental Research Funds for the Central Universities(2023CDJKYJH103)
Year 2026 volume 18 Issue 5
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Article Info
doi: 10.1016/j.jrmge.2025.06.035
  • Receive Date:2025-03-05
  • Online Date:2026-06-17
  • Published:2026-05-25
Article Data
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History
  • Received:2025-03-05
  • Revised:2025-06-02
  • Accepted:2025-06-02
Funding
National Natural Science Foundation of China(52425806; 52378325)
Fundamental Research Funds for the Central Universities(2023CDJKYJH103)
Affiliations
    aKey Laboratory of New Technology for Construction of Cities in Mountain Area, Chongqing University, Chongqing, 400045, China
    bSchool of Civil and Environmental Engineering, Nanyang Technological University, 639798, Singapore

Corresponding:

* Corresponding author. E-mail address: (C. Zhao).
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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