Xyloglucan represents the primary hemicellulose component in higher plant cell walls, providing mechanical support. The XTH gene family encodes xyloglucan endotransferase/hydrolase, a crucial enzyme in cell wall remodeling. Studies examining XTH family-related genes in apples remain limited. This studyinvestigated the MdXTH30gene, isolated from apple (Malus×domestica), which demonstrated responsiveness to abscisic acid, NaCl, and polyethylene glycol (PEG) 6000, with cytoplasmic localization confirmed through subcellular mapping. To elucidate the role of MdXTH30in stress response, transgenic MdXTH30apple calli were generated andthe gene was heterologously expressed in ArabidopsisviaAgrobacterium-mediated transformation. The findings revealed that MdXTH30enhanced resistance to drought, salt stress, and pathogens through regulation of relevant genes inboth apple calli and Arabidopsis. These results identify potentially significant candidate genes for improving biotic and abiotic stress resistance at the cell wall level.

tomato  /  abscission  /  SlHSL  /  SlIDL6  /  Ethylene