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A rapid and visual detection method for porcine deltacoronavirus with single-copy sensitivity based on the CRISPR/Cas12a assay
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Journal of Integrative Agriculture | 2026, 25(4) : 1736 - 1739
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Journal of Integrative Agriculture | 2026, 25(4): 1736-1739
• Letter •
A rapid and visual detection method for porcine deltacoronavirus with single-copy sensitivity based on the CRISPR/Cas12a assay
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Zezhao Cao1*, Zi Li1*, Kaikai Jin1, Zanheng Huang1, Ruijie Hu1, Junchao Shi1, Huabo Yu1, Gaili Wang2, Wenqi He1, Hualei Wang1, Yungang Lan1#, Haili Zhang1#
Affiliations
    1State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China
    2Jilin Academy of Animal Husbandry and Veterinary Medicine, Changchun 130062, China
About Author:
Zezhao Cao, E-mail: 599142457@qq.com; Zi Li, E-mail: lizi1227@jlu.edu.cn; #Correspondence Haili Zhang, E-mail: zhanghaili@jlu.edu.cn; Yungang Lan, E-mail: lanyungang@jlu.edu.cn * These authors contributed equally to this study.
doi: 10.1016/j.jia.2025.07.030
Outline
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. 一种基于 CRISPR/Cas12a单拷贝灵敏度的猪德尔塔冠状病毒快速可视化检测方法的建立. 农业科学学报（英文版）, 2026 , 25 (4) : 1736 -1739 . DOI: 10.1016/j.jia.2025.07.030
Zezhao Cao, Zi Li, Kaikai Jin, Zanheng Huang, Ruijie Hu, Junchao Shi, Huabo Yu, Gaili Wang, Wenqi He, Hualei Wang, Yungang Lan, Haili Zhang. A rapid and visual detection method for porcine deltacoronavirus with single-copy sensitivity based on the CRISPR/Cas12a assay[J]. Journal of Integrative Agriculture, 2026 , 25 (4) : 1736 -1739 . DOI: 10.1016/j.jia.2025.07.030
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Year 2026 volume 25 Issue 4
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doi: 10.1016/j.jia.2025.07.030
  • Online Date：2026-10-27
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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