The strawberry crimp nematode (Aphelenchoidesfragariae) is a serious pathogen of ornamental crops and a significant quarantine concern in approximately 50 countries and regions, including China. A nematode population belonging to the genus Aphelenchoideswas isolated from symptomatic leaves of fuchsia plants (Fuchsia×hybridaHort. ex Sieb. & Voss.) in Chengdu, Sichuan Province, China. Morphological and morphometric characteristics were determined using light microscopy and scanning electron microscopy. Detailed examination revealed diagnostic features consistent with A.fragariae. Three ribosomal DNA (rDNA) regions, i.e., partial small subunit(SSU) rRNA, D2-D3 expansion segments of the large subunit (LSU) rRNA, andthe internal transcribed spacer (ITS), were amplified and sequenced. Bayesian phylogenetic analyses based on these sequences placed the isolate in a well-supported monophyletic clade withreference A.fragariaespecimens, clearly separated from other Aphelenchoidesspecies. Furthermore, host-suitability assays demonstrated that this nematode population not only infects and reproduces on Fuchsia×hybrida, but also on Fragariaananassaand Pterisvittata, two known hosts of A.fragariae. Collectively, morphological,molecular, and host-range evidence confirm the identification of this nematode as A.fragariae. To our knowledge, this represents the first molecular and morphological confirmation of A.fragariaein China, and the first report ofFuchsia×hybridaas a natural host for this species.

rice disease diagnosis  /  ensemble method  /  CNN-based model  /  end-to-end model  /  Inception model  /  DenseNet model  /  vision transformer model  /  attention-based model  /  support vector machine