Article(id=1207394340456993762, tenantId=1146029695717560320, journalId=1189873630562394117, issueId=1207394339840431074, articleNumber=null, orderNo=null, doi=10.11855/j.issn.0577-7402.2022.10.0968, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1652976000000, receivedDateStr=2022-05-20, revisedDate=null, revisedDateStr=null, acceptedDate=1657296000000, acceptedDateStr=2022-07-09, onlineDate=1765794843067, onlineDateStr=2025-12-15, pubDate=1666886400000, pubDateStr=2022-10-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1765794843067, onlineIssueDateStr=2025-12-15, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1765794843067, creator=13701087609, updateTime=1765794843067, updator=13701087609, issue=Issue{id=1207394339840431074, tenantId=1146029695717560320, journalId=1189873630562394117, year='2022', volume='47', issue='10', pageStart='957', pageEnd='1062', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=0, createTime=1765794842920, creator=13701087609, updateTime=1765794898634, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1207394573588992611, tenantId=1146029695717560320, journalId=1189873630562394117, issueId=1207394339840431074, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1207394573588992612, tenantId=1146029695717560320, journalId=1189873630562394117, issueId=1207394339840431074, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=968, endPage=975, ext={EN=ArticleExt(id=1207394341534929892, articleId=1207394340456993762, tenantId=1146029695717560320, journalId=1189873630562394117, language=EN, title=Effects of miR-203 on the biological characteristics of HPV16-positive cervical cancer cells by targeted-regulating CUL2, columnId=1190310110212751762, journalTitle=Medical Journal of Chinese People’s Liberation Army, columnName=Basic Research, runingTitle=null, highlight=null, articleAbstract=

Objective To explore the effect of hsa-microRNA-203-3p (miR-203) targeted-regulating Cullin2 (CUL2) on the biological characteristics of human papillomavirus 16 (HPV16) positive cervical cancer cells. Methods A total of 10 patients underwent cervical cancer screening from September 2018 to September 2019 in the Department of Obstetrics and Gynecology, the Second Hospital of Shanxi Medical University. HPV genotyping was performed as single HPV16 positive, and pathological examination showed cervical squamous cell carcinoma (SCC). Ten corresponding paracancer normal tissue samples were collected as control group. Real-time quantitative reverse transcription PCR (qRT-PCR) was performed to detect the expression of miR-203 in cervical squamous cell carcinoma and corresponding adjacent tissues, cervical cancer cell line (SiHa) and human immortal keratinocyte line (HaCaT) cells. GO and KEGG enrichment were applied to analyze the functions and pathways miR-203 involved. The regulatory relationship between miR-203 and CUL2 were verified via TargetScan website and dual luciferase reporter assay. The miR-203 mimics or inhibitor were transfected into SiHa cells to establish cell models of high and low expression of miR-203,and the expressions of CUL2 mRNA and protein were detected by qRT-PCR and Western blotting. The proliferation, migration and apoptosis of SiHa cells were assessed by CCK-8, scratch assay and Annexin V-APC/PI double staining, respectively. Results Results of qRT-PCR indicated that, compared with the corresponding adjacent tissues and HaCaT cells, the relative expression level of miR-203 decreased obviously in both cervical SCC tissues and SiHa cells (P<0.01). The results of GO and KEGG enrichment methods showed that miR-203 was widely involved in the ubiquitination process and the signaling pathways involved in the malignancies. TargetScan website and dual-luciferase reporter assay showed that the targeting regulatory relationship existed between miR-203 and CUL2 (P<0.01). qRT-PCR and Western blotting indicated that overexpression of miR-203 reduced the expressions of CUL2 mRNA and protein (P<0.05 or P<0.01); While low expression of miR-203 up-regulated the expressions of CUL2 mRNA and protein (P<0.01). CCK-8, scratch experiments and Annexin V-APC/PI double staining method confirmed that overexpression of miR-203 decreased proliferation rate and migration rate of SiHa cells (P<0.01), and elevated cell apoptosis rate (P<0.05). In contrast, low expression of miR-203 increased the proliferation rate and migration rate of SiHa cells (P<0.01), and reduced the apoptosis rate(P<0.01). Conclusion The miR-203 might suppress the biological characteristics of HPV16-positive cervical cancer cells SiHa by targeting CUL2, and it was expected to become a new candidate gene in diagnosis and treatment of cervical cancer.

, correspAuthors=Wei-Hong Zhao, authorNote=null, correspAuthorsNote=, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Ya-Qin Li, Ya-Tao Liu, Hong-Lei Zhang, Wei-Hong Zhao), CN=ArticleExt(id=1207394342302487540, articleId=1207394340456993762, tenantId=1146029695717560320, journalId=1189873630562394117, language=CN, title=miR-203靶向调控CUL2对HPV16阳性宫颈癌细胞生物学特性的影响, columnId=1190310110472798614, journalTitle=解放军医学杂志, columnName=基础研究, runingTitle=null, highlight=null, articleAbstract=

目的 探讨微小RNA 203-3p(miR-203)靶向调控枯灵素2(CUL2)对人乳头瘤病毒16型(HPV16)阳性宫颈癌细胞生物学特性的影响。方法 收集2018年9月－2019年9月于山西医科大学第二医院妇产科行宫颈癌筛查，HPV检测为单一HPV16阳性，病理检查为宫颈鳞癌(SCC)的样本及相应癌旁组织(对照组)各10份。采用实时定量反转录聚合酶链反应(qRT-PCR)检测SCC与相应癌旁组织，以及人SCC细胞(SiHa)与人永生化表皮细胞(HaCaT)中miR-203的表达。采用GO和KEGG富集法分析miR-203参与的功能和通路，TargetScan网站及双荧光素酶报告基因实验验证miR-203与CUL2的靶向调控作用。将miR-203模拟物或抑制剂转染至SiHa细胞，建立过表达或低表达miR-203的细胞模型，采用qRT-PCR和Western blotting检测CUL2 mRNA及蛋白的表达；采用CCK-8法、划痕实验、AnnexinⅤ-APC/PI双染色法评估SiHa细胞的增殖、迁移和凋亡能力。结果 qRT-PCR检测分析结果显示，与相应癌旁组织和HaCaT细胞相比，miR-203在SCC组织和SiHa细胞中的表达水平均明显降低(P<0.01)。GO和KEGG富集结果显示，miR-203广泛参与泛素化过程并与恶性肿瘤参与的信号通路有关。TargetScan与双荧光素酶报告基因实验表明，miR-203与CUL2存在靶向调控关系(P<0.01)。qRT-PCR及Western blotting结果表明，过表达miR-203时，CUL2 mRNA及蛋白表达水平均明显降低(P<0.05或P<0.01)，而低表达miR-203则可上调CUL2 mRNA及蛋白的表达(P<0.01)。CCK-8、划痕实验和Annexin Ⅴ-APC/PI双染结果显示，过表达miR-203可降低SiHa细胞的增殖率和迁移率(P<0.01)，升高细胞凋亡率(P<0.05)；相反，低表达miR-203可升高SiHa细胞的增殖率和迁移率(P<0.01)，降低细胞凋亡率(P<0.01)。结论 miR-203可能通过靶向CUL2抑制HPV16阳性宫颈癌细胞SiHa的生物学特性，有望成为宫颈癌诊断和治疗的一个新的候选基因。

, correspAuthors=赵卫红, authorNote=null, correspAuthorsNote=, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=QxzAdkzvicY0c78MW2c82w==, magXml=0qoMpmdGkS7yn5H2XTTGtw==, pdfUrl=null, pdf=6B22QaJIfaQE0jqW55YzNg==, pdfFileSize=8671135, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=BatbyIsDI+RAVqZtSP2CBA==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=96k4PzUIBtF23BTqlAAYYg==, mapNumber=null, authorCompany=null, fund=null, authors=, authorsList=李亚芹, 刘亚涛, 张宏蕾, 赵卫红)}, authors=[Author(id=1207394342805803017, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1207394342872911887, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, authorId=1207394342805803017, language=EN, stringName=Ya-Qin Li, firstName=Ya-Qin, middleName=null, lastName=Li, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=^{1, 2}, address=¹Department of Obstetrics and Gynecology, the Second Clinical Medical College, Shanxi Medical University, Taiyuan, Shanxi 030001, China
²Department of Obstetrics and Gynecology, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1207394342952603667, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, authorId=1207394342805803017, language=CN, stringName=李亚芹, firstName=亚芹, middleName=null, lastName=李, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=^{1, 2}, address=¹山西医科大学第二临床医学院妇产科，山西太原 030001
²山西医科大学第二医院妇产科，山西太原 030001, bio={"content":"

李亚芹，硕士研究生，主要从事妇科肿瘤发病机制方面的研究

"}, bioImg=null, bioContent=

李亚芹，硕士研究生，主要从事妇科肿瘤发病机制方面的研究

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1207394342499619832, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, xref=1, ext=[AuthorCompanyExt(id=1207394342508008441, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342499619832, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=¹Department of Obstetrics and Gynecology, the Second Clinical Medical College, Shanxi Medical University, Taiyuan, Shanxi 030001, China), AuthorCompanyExt(id=1207394342516397050, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342499619832, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=¹山西医科大学第二临床医学院妇产科，山西太原 030001)]), AuthorCompany(id=1207394342575117309, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, xref=2, ext=[AuthorCompanyExt(id=1207394342583505918, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342575117309, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=²Department of Obstetrics and Gynecology, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China), AuthorCompanyExt(id=1207394342591894528, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342575117309, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=²山西医科大学第二医院妇产科，山西太原 030001)])]), Author(id=1207394343053266969, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1207394343208456233, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, authorId=1207394343053266969, language=EN, stringName=Ya-Tao Liu, firstName=Ya-Tao, middleName=null, lastName=Liu, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=^{1, 2}, address=¹Department of Obstetrics and Gynecology, the Second Clinical Medical College, Shanxi Medical University, Taiyuan, Shanxi 030001, China
²Department of Obstetrics and Gynecology, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1207394343271370798, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, authorId=1207394343053266969, language=CN, stringName=刘亚涛, firstName=亚涛, middleName=null, lastName=刘, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=^{1, 2}, address=¹山西医科大学第二临床医学院妇产科，山西太原 030001
²山西医科大学第二医院妇产科，山西太原 030001, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1207394342499619832, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, xref=1, ext=[AuthorCompanyExt(id=1207394342508008441, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342499619832, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=¹Department of Obstetrics and Gynecology, the Second Clinical Medical College, Shanxi Medical University, Taiyuan, Shanxi 030001, China), AuthorCompanyExt(id=1207394342516397050, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342499619832, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=¹山西医科大学第二临床医学院妇产科，山西太原 030001)]), AuthorCompany(id=1207394342575117309, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, xref=2, ext=[AuthorCompanyExt(id=1207394342583505918, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342575117309, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=²Department of Obstetrics and Gynecology, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China), AuthorCompanyExt(id=1207394342591894528, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342575117309, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=²山西医科大学第二医院妇产科，山西太原 030001)])]), Author(id=1207394343355256882, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1207394343422365751, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, authorId=1207394343355256882, language=EN, stringName=Hong-Lei Zhang, firstName=Hong-Lei, middleName=null, lastName=Zhang, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=³, address=³Department of Pathology and Pathophysiology, Basic Medical College, Shanxi Medical University, Taiyuan, Shanxi 030001, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1207394343518834746, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, authorId=1207394343355256882, language=CN, stringName=张宏蕾, firstName=宏蕾, middleName=null, lastName=张, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=³, address=³山西医科大学基础医学院病理学与病理生理学系，山西太原 030001, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1207394342726111235, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, xref=3, ext=[AuthorCompanyExt(id=1207394342734499844, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342726111235, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=³Department of Pathology and Pathophysiology, Basic Medical College, Shanxi Medical University, Taiyuan, Shanxi 030001, China), AuthorCompanyExt(id=1207394342742888453, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342726111235, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=³山西医科大学基础医学院病理学与病理生理学系，山西太原 030001)])]), Author(id=1207394343653052482, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=sydeyzwh@sxmu.edu.cn, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1207394343770493001, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, authorId=1207394343653052482, language=EN, stringName=Wei-Hong Zhao, firstName=Wei-Hong, middleName=null, lastName=Zhao, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=^{2, *}, address=²Department of Obstetrics and Gynecology, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1207394343858573393, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, authorId=1207394343653052482, language=CN, stringName=赵卫红, firstName=卫红, middleName=null, lastName=赵, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=^{2, *}, address=²山西医科大学第二医院妇产科，山西太原 030001, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1207394342575117309, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, xref=2, ext=[AuthorCompanyExt(id=1207394342583505918, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342575117309, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=²Department of Obstetrics and Gynecology, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China), AuthorCompanyExt(id=1207394342591894528, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342575117309, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=²山西医科大学第二医院妇产科，山西太原 030001)])])], keywords=[Keyword(id=1207394344043122777, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=EN, orderNo=1, keyword=cervical cancer), Keyword(id=1207394344152174692, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=EN, orderNo=2, keyword=hsa-microRNA-203-3p), Keyword(id=1207394344298975338, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=EN, orderNo=3, keyword=Cullin2), Keyword(id=1207394344399638640, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=EN, orderNo=4, keyword=human papillomavirus 16), Keyword(id=1207394344504496252, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=CN, orderNo=1, keyword=宫颈癌), Keyword(id=1207394344621936773, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=CN, orderNo=2, keyword=微小RNA 203-3p), Keyword(id=1207394345834090639, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=CN, orderNo=3, keyword=枯灵素2), Keyword(id=1207394345905393815, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=CN, orderNo=4, keyword=人乳头瘤病毒16型)], refs=[Reference(id=1207394348006740268, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2021, volume=71, issue=3, pageStart=209, pageEnd=249, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=Sung H, Ferlay J, Siegel RL, journalName=CA Cancer J Clin, refType=null, unstructuredReference=Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71(3): 209-249., articleTitle=Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries, refAbstract=null), Reference(id=1207394348149346610, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2021, volume=13, issue=2, pageStart=321, pageEnd=null, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=Della Fera AN, Warburton A, Coursey TL, journalName=Viruses, refType=null, unstructuredReference=Della Fera AN, Warburton A, Coursey TL, et al. Persistent human papillomavirus infection[J]. Viruses, 2021, 13(2): 321., articleTitle=Persistent human papillomavirus infection, refAbstract=null), Reference(id=1207394348266787130, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2020, volume=45, issue=7, pageStart=691, pageEnd=696, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=Zhao XY, Zhang M, Zhu ZH, journalName=Med J Chin PLA, refType=null, unstructuredReference=Zhao XY, Zhang M, Zhu ZH, et al. Effect and mechanism of 6-gingerol on invasion and migration of HPV-positive and negative cervical cancer cells[J]. Med J Chin PLA, 2020, 45(7):691-696., articleTitle=Effect and mechanism of 6-gingerol on invasion and migration of HPV-positive and negative cervical cancer cells, refAbstract=null), Reference(id=1207394348388421951, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2020, volume=null, issue=7, pageStart=691, pageEnd=696, url=null, language=null, rfNumber=[3], rfOrder=3, authorNames=赵行宇, 张漠, 朱志华, journalName=解放军医学杂志, refType=null, unstructuredReference=[赵行宇, 张漠, 朱志华, 等. 6-姜烯酚对HPV阳性及阴性宫颈癌细胞侵袭及迁移的作用及其机制[J]. 解放军医学杂志, 2020, 45(7): 691-696.], articleTitle=6-姜烯酚对HPV阳性及阴性宫颈癌细胞侵袭及迁移的作用及其机制, refAbstract=null), Reference(id=1207394348476502338, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2022, volume=174, issue=null, pageStart=103675, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=4, authorNames=Bhattacharjee R, Das SS, Biswal SS, journalName=Crit Rev Oncol Hematol, refType=null, unstructuredReference=Bhattacharjee R, Das SS, Biswal SS, et al. Mechanistic role of HPV-associated early proteins in cervical cancer: molecular pathways and targeted therapeutic strategies[J]. Crit Rev Oncol Hematol, 2022, 174: 103675., articleTitle=Mechanistic role of HPV-associated early proteins in cervical cancer: molecular pathways and targeted therapeutic strategies, refAbstract=null), Reference(id=1207394348589748557, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2021, volume=null, issue=6, pageStart=S1044-579X(21)00161-9, pageEnd=null, url=null, language=null, rfNumber=[5], rfOrder=5, authorNames=Khan A, Das BC, Abiha U, journalName=Semin Cancer Biol, refType=null, unstructuredReference=Khan A, Das BC, Abiha U, et al. Insights into the role of complement regulatory proteins in HPV mediated cervical carcinogenesis[J]. Semin Cancer Biol, 2021(6): S1044-579X(21)00161-9., articleTitle=Insights into the role of complement regulatory proteins in HPV mediated cervical carcinogenesis, refAbstract=null), Reference(id=1207394348711383379, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2021, volume=77, issue=4, pageStart=547, pageEnd=555, url=null, language=null, rfNumber=[6], rfOrder=6, authorNames=Ocadiz-Delgado R, Cruz-Colin JL, Alvarez-Rios E, journalName=J Physiol Biochem, refType=null, unstructuredReference=Ocadiz-Delgado R, Cruz-Colin JL, Alvarez-Rios E, et al. Expression of miR-34a and miR-15b during the progression of cervical cancer in a murine model expressing the HPV16 E7 oncoprotein[J]. J Physiol Biochem, 2021, 77(4): 547-555., articleTitle=Expression of miR-34a and miR-15b during the progression of cervical cancer in a murine model expressing the HPV16 E7 oncoprotein, refAbstract=null), Reference(id=1207394348853989721, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2016, volume=11, issue=6, pageStart=3863, pageEnd=3869, url=null, language=null, rfNumber=[7], rfOrder=7, authorNames=Hao M, Zhao WH, Zhang LL, journalName=Oncol Lett, refType=null, unstructuredReference=Hao M, Zhao WH, Zhang LL, et al. Low folate levels are associated with methylation-mediated transcriptional repression of miR-203 and miR-375 during cervical carcinogenesis[J]. Oncol Lett, 2016, 11(6): 3863-3869., articleTitle=Low folate levels are associated with methylation-mediated transcriptional repression of miR-203 and miR-375 during cervical carcinogenesis, refAbstract=null), Reference(id=1207394348950458718, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2016, volume=166, issue=5, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[8], rfOrder=8, authorNames=Scott DC, Rhee DY, Duda DM, journalName=Cell, refType=null, unstructuredReference=Scott DC, Rhee DY, Duda DM, et al. Two distinct types of E3 ligases work in unison to regulate substrate ubiquitylation[J]. Cell, 2016, 166(5): 1198-1214, e24., articleTitle=Two distinct types of E3 ligases work in unison to regulate substrate ubiquitylation, refAbstract=null), Reference(id=1207394349038539108, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2008, volume=15, issue=7, pageStart=1187, pageEnd=1195, url=null, language=null, rfNumber=[9], rfOrder=9, authorNames=Lena AM, Shalom-Feuerstein R, di Val Cervo PR, journalName=Cell Death Differ, refType=null, unstructuredReference=Lena AM, Shalom-Feuerstein R, di Val Cervo PR, et al. miR-203 represses 'stemness' by repressing ΔNp63[J]. Cell Death Differ, 2008, 15(7): 1187-1195., articleTitle=miR-203 represses 'stemness' by repressing ΔNp63, refAbstract=null), Reference(id=1207394350267470186, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2020, volume=20, issue=1, pageStart=1028, pageEnd=null, url=null, language=null, rfNumber=[10], rfOrder=10, authorNames=Meng Y, Hu X, Li S, journalName=BMC Cancer, refType=null, unstructuredReference=Meng Y, Hu X, Li S, et al. miR-203 inhibits cell proliferation and ERK pathway in prostate cancer by targeting IRS-1[J]. BMC Cancer, 2020, 20(1): 1028., articleTitle=miR-203 inhibits cell proliferation and ERK pathway in prostate cancer by targeting IRS-1, refAbstract=null), Reference(id=1207394350376522096, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2021, volume=35, issue=3, pageStart=1109, pageEnd=1115, url=null, language=null, rfNumber=[11], rfOrder=11, authorNames=Wang SL, Dong XW, Zhao F, journalName=J Biol Regul Homeost Agents, refType=null, unstructuredReference=Wang SL, Dong XW, Zhao F, et al. MiR-203 inhibits cell proliferation, invasion, and migration of ovarian cancer through regulating RGS17[J]. J Biol Regul Homeost Agents, 2021, 35(3): 1109-1115., articleTitle=MiR-203 inhibits cell proliferation, invasion, and migration of ovarian cancer through regulating RGS17, refAbstract=null), Reference(id=1207394350452019574, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2011, volume=4, issue=10, pageStart=1698, pageEnd=1709, url=null, language=null, rfNumber=[12], rfOrder=12, authorNames=Saini S, Arora S, Majid S, journalName=Cancer Prev Res (Phila), refType=null, unstructuredReference=Saini S, Arora S, Majid S, et al. Curcumin modulates microRNA-203-mediated regulation of the Src-Akt axis in bladder cancer[J]. Cancer Prev Res (Phila), 2011, 4(10): 1698-1709., articleTitle=Curcumin modulates microRNA-203-mediated regulation of the Src-Akt axis in bladder cancer, refAbstract=null), Reference(id=1207394350577848700, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2011, volume=278, issue=5, pageStart=786, pageEnd=792, url=null, language=null, rfNumber=[13], rfOrder=13, authorNames=Bo JJ, Yang GL, Huo KL, journalName=FEBS J, refType=null, unstructuredReference=Bo JJ, Yang GL, Huo KL, et al. microRNA-203 suppresses bladder cancer development by repressing bcl-w expression[J]. FEBS J, 2011, 278(5): 786-792., articleTitle=microRNA-203 suppresses bladder cancer development by repressing bcl-w expression, refAbstract=null), Reference(id=1207394350720455038, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2018, volume=26, issue=8, pageStart=1155, pageEnd=1165, url=null, language=null, rfNumber=[14], rfOrder=14, authorNames=Shen J, Zhang JH, Xiao MH, journalName=Oncol Res, refType=null, unstructuredReference=Shen J, Zhang JH, Xiao MH, et al. miR-203 suppresses bladder cancer cell growth and targets Twist1[J]. Oncol Res, 2018, 26(8): 1155-1165., articleTitle=miR-203 suppresses bladder cancer cell growth and targets Twist1, refAbstract=null), Reference(id=1207394350783369603, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2022, volume=41, issue=null, pageStart=9603271211062854, pageEnd=null, url=null, language=null, rfNumber=[15], rfOrder=15, authorNames=Wang S, Zhang C, Chen R, journalName=Hum Exp Toxicol, refType=null, unstructuredReference=Wang S, Zhang C, Chen R. Circ_0006220 promotes non-small cell lung cancer progression via sponging miR-203-3p and regulating RGS17 expression[J]. Hum Exp Toxicol, 2022, 41:9603271211062854., articleTitle=Circ_0006220 promotes non-small cell lung cancer progression via sponging miR-203-3p and regulating RGS17 expression, refAbstract=null), Reference(id=1207394350871449993, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2013, volume=8, issue=2, pageStart=220, pageEnd=228, url=null, language=null, rfNumber=[16], rfOrder=16, authorNames=Wilting SM, Verlaat W, Jaspers A, journalName=Epigenetics, refType=null, unstructuredReference=Wilting SM, Verlaat W, Jaspers A, et al. Methylation-mediated transcriptional repression of microRNAs during cervical carcinogenesis[J]. Epigenetics, 2013, 8(2): 220-228., articleTitle=Methylation-mediated transcriptional repression of microRNAs during cervical carcinogenesis, refAbstract=null), Reference(id=1207394350959530381, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2019, volume=12, issue=1, pageStart=92, pageEnd=null, url=null, language=null, rfNumber=[17], rfOrder=17, authorNames=Zheng ZH, Wu DM, Fan SH, journalName=J Ovarian Res, refType=null, unstructuredReference=Zheng ZH, Wu DM, Fan SH, et al. LncRNA AB209371 up-regulated survivin gene by down-regulating miR-203 in ovarian carcinoma[J]. J Ovarian Res, 2019, 12(1): 92., articleTitle=LncRNA AB209371 up-regulated survivin gene by down-regulating miR-203 in ovarian carcinoma, refAbstract=null), Reference(id=1207394351060193681, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2021, volume=2021, issue=null, pageStart=4190764, pageEnd=null, url=null, language=null, rfNumber=[18], rfOrder=18, authorNames=Zhang W, Liu J, Wu Q, journalName=J Oncol, refType=null, unstructuredReference=Zhang W, Liu J, Wu Q, et al. HOTAIR contributes to stemness acquisition of cervical cancer through regulating miR-203 interaction with ZEB1 on epithelial-mesenchymal transition[J]. J Oncol, 2021, 2021: 4190764., articleTitle=HOTAIR contributes to stemness acquisition of cervical cancer through regulating miR-203 interaction with ZEB1 on epithelial-mesenchymal transition, refAbstract=null), Reference(id=1207394351165051286, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2020, volume=20, issue=null, pageStart=355, pageEnd=null, url=null, language=null, rfNumber=[19], rfOrder=19, authorNames=Li G, Qi HW, Dong HG, journalName=Cancer Cell Int, refType=null, unstructuredReference=Li G, Qi HW, Dong HG, et al. Targeting deubiquitinating enzyme USP26 by microRNA-203 regulates Snail1's pro-metastatic functions in esophageal cancer[J]. Cancer Cell Int, 2020, 20:355., articleTitle=Targeting deubiquitinating enzyme USP26 by microRNA-203 regulates Snail1's pro-metastatic functions in esophageal cancer, refAbstract=null), Reference(id=1207394351278297500, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2016, volume=7, issue=29, pageStart=46707, pageEnd=46716, url=null, language=null, rfNumber=[20], rfOrder=20, authorNames=Wang SW, Xia WJ, Qiu MT, journalName=Oncotarget, refType=null, unstructuredReference=Wang SW, Xia WJ, Qiu MT, et al. Atlas on substrate recognition subunits of CRL2 E3 ligases[J]. Oncotarget, 2016, 7(29):46707-46716., articleTitle=Atlas on substrate recognition subunits of CRL2 E3 ligases, refAbstract=null), Reference(id=1207394351391543710, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2016, volume=7, issue=21, pageStart=31520, pageEnd=31533, url=null, language=null, rfNumber=[21], rfOrder=21, authorNames=Xu JF, Fang YF, Wang XY, journalName=Oncotarget, refType=null, unstructuredReference=Xu JF, Fang YF, Wang XY, et al. CUL2 overexpression driven by CUL2/E2F1/miR-424 regulatory loop promotes HPV16 E7 induced cervical carcinogenesis[J]. Oncotarget, 2016, 7(21):31520-31533., articleTitle=CUL2 overexpression driven by CUL2/E2F1/miR-424 regulatory loop promotes HPV16 E7 induced cervical carcinogenesis, refAbstract=null), Reference(id=1207394351521567141, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2007, volume=81, issue=18, pageStart=9737, pageEnd=9747, url=null, language=null, rfNumber=[22], rfOrder=22, authorNames=Huh K, Zhou X, Hayakawa H, journalName=J Virol, refType=null, unstructuredReference=Huh K, Zhou X, Hayakawa H, et al. Human papillomavirus type 16 E7 oncoprotein associates with the cullin 2 ubiquitin ligase complex, which contributes to degradation of the retinoblastoma tumor suppressor[J]. J Virol, 2007, 81(18): 9737-9747., articleTitle=Human papillomavirus type 16 E7 oncoprotein associates with the cullin 2 ubiquitin ligase complex, which contributes to degradation of the retinoblastoma tumor suppressor, refAbstract=null), Reference(id=1207394351630619049, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, doi=null, pmid=null, pmcid=null, year=2020, volume=11, issue=18, pageStart=5379, pageEnd=5389, url=null, language=null, rfNumber=[23], rfOrder=23, authorNames=Zhao WH, Liu YT, Zhang LL, journalName=J Cancer, refType=null, unstructuredReference=Zhao WH, Liu YT, Zhang LL, et al. MicroRNA-154-5p regulates the HPV16 E7-pRb pathway in Cervical Carcinogenesis by targeting CUL2[J]. J Cancer, 2020, 11(18): 5379-5389., articleTitle=MicroRNA-154-5p regulates the HPV16 E7-pRb pathway in Cervical Carcinogenesis by targeting CUL2, refAbstract=null)], funds=[Fund(id=1207394347117547760, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, awardId=81702583, language=EN, fundingSource=National Natural Science Foundation of China(81702583), fundOrder=null, country=null), Fund(id=1207394347205628151, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, awardId=81702583, language=CN, fundingSource=国家自然科学基金(81702583), fundOrder=null, country=null), Fund(id=1207394347327262975, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, awardId=201701D31111191, language=EN, fundingSource=Science and Technology Innovation Project of Higher Education in Shanxi Province(201701D31111191), fundOrder=null, country=null), Fund(id=1207394347436314884, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, awardId=201701D31111191, language=CN, fundingSource=山西省高等学校科技创新项目(201701D31111191), fundOrder=null, country=null), Fund(id=1207394347536978187, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, awardId=201901D211506, language=EN, fundingSource=Outstanding Youth Fund Project of Shanxi Province(201901D211506), fundOrder=null, country=null), Fund(id=1207394347629252884, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, awardId=201901D211506, language=CN, fundingSource=山西省优秀青年基金项目(201901D211506), fundOrder=null, country=null), Fund(id=1207394347725721881, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, awardId=2022-195, language=EN, fundingSource=Research Project Supported by Shanxi Scholarship Council of China(2022-195), fundOrder=null, country=null), Fund(id=1207394347830579487, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, awardId=2022-195, language=CN, fundingSource=山西省回国留学人员科研教研资助项目(2022-195), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1207394342499619832, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, xref=1, ext=[AuthorCompanyExt(id=1207394342508008441, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342499619832, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=¹Department of Obstetrics and Gynecology, the Second Clinical Medical College, Shanxi Medical University, Taiyuan, Shanxi 030001, China), AuthorCompanyExt(id=1207394342516397050, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342499619832, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=¹山西医科大学第二临床医学院妇产科，山西太原 030001)]), AuthorCompany(id=1207394342575117309, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, xref=2, ext=[AuthorCompanyExt(id=1207394342583505918, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342575117309, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=²Department of Obstetrics and Gynecology, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China), AuthorCompanyExt(id=1207394342591894528, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342575117309, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=²山西医科大学第二医院妇产科，山西太原 030001)]), AuthorCompany(id=1207394342726111235, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, xref=3, ext=[AuthorCompanyExt(id=1207394342734499844, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342726111235, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=³Department of Pathology and Pathophysiology, Basic Medical College, Shanxi Medical University, Taiyuan, Shanxi 030001, China), AuthorCompanyExt(id=1207394342742888453, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, companyId=1207394342726111235, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=³山西医科大学基础医学院病理学与病理生理学系，山西太原 030001)])], figs=[ArticleFig(id=1207394346110914725, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=EN, label=Fig. 1, caption=Function, pathway enrichment and targeting regulatory relationship with CUL2 of miR-203, figureFileSmall=KdYV+t0W/UUp41NSlyfp6g==, figureFileBig=BatbyIsDI+RAVqZtSP2CBA==, tableContent=null), ArticleFig(id=1207394346194800814, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=CN, label=图1, caption=miR-203的功能、通路富集及与CUL2的靶向关系

A. GO富集分析；B. KEGG富集分析；C. miR-203与CUL2的结合位点；D. miR-203与CUL2的靶向关系；(1)P<0.01

, figureFileSmall=KdYV+t0W/UUp41NSlyfp6g==, figureFileBig=BatbyIsDI+RAVqZtSP2CBA==, tableContent=null), ArticleFig(id=1207394346396127416, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=EN, label=Fig. 2, caption=Effect of overexpression and low expression of miR-203 on CUL2 mRNA and protein, figureFileSmall=etySWOZFdJbjMe7PC5/obA==, figureFileBig=Igjlq2WlSE8qikxTYulQOg==, tableContent=null), ArticleFig(id=1207394346488402112, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=CN, label=图2, caption=过表达和低表达miR-203对CUL2 mRNA及蛋白表达的影响

A. qRT-PCR检测HaCaT和SiHa细胞中CUL2 mRNA的表达情况；B. 过表达和低表达miR-203的SiHa细胞普通光学显微镜及倒置荧光显微镜成像结果；C. qRT-PCR检测过表达和低表达miR-203的SiHa细胞miR-203及CUL2 mRNA的相对表达水平；D. Western blotting检测过表达和低表达miR-203的SiHa细胞CUL2蛋白的相对表达水平；(1)P<0.05，(2)P<0.01

, figureFileSmall=etySWOZFdJbjMe7PC5/obA==, figureFileBig=Igjlq2WlSE8qikxTYulQOg==, tableContent=null), ArticleFig(id=1207394346555510983, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=EN, label=Fig. 3, caption=Effect pf overexpression and low expression of miR-203 on proliferation, migration and apoptosis of SiHa cells, figureFileSmall=a31lk6yj8/7ze5mOPmSERA==, figureFileBig=D/lVA5ff/b+dNRBYkP3A7w==, tableContent=null), ArticleFig(id=1207394346618425550, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=CN, label=图3, caption=过表达和低表达miR-203对SiHa细胞的增殖、迁移和凋亡的影响

A. CCK-8法检测细胞增殖率；B. 划痕实验测定细胞迁移率；C. 细胞凋亡实验检测细胞凋亡率；(1)P<0.05，(2)P<0.01

, figureFileSmall=a31lk6yj8/7ze5mOPmSERA==, figureFileBig=D/lVA5ff/b+dNRBYkP3A7w==, tableContent=null), ArticleFig(id=1207394346710700242, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=EN, label=Tab. 1, caption=

miR-203 sequence for transfection experiment

, figureFileSmall=null, figureFileBig=null, tableContent=

基因	序列
miR-203 mimics	正义：5'-GUGAAAUGUUUAGGACCACUAG-3'
	反义：5'-AGUGGGUCCUAAACAUUUCACUU-3'
miR-203 mimics NC	正义：5'-UUCUCCGAACGUGUCACGUTT-3'
	反义：5'-ACGUGACACGUUCGGAGAATT-3'
miR-203 inhibitor	5'-CUAGUGGUCCUAAACAUUUCAC-3'
miR-203 inhibitor	NC 5'-CAGUACUUUUGUGUAGUACAA-3'

), ArticleFig(id=1207394346802974938, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=CN, label=表1, caption=

miR-203转染片段序列

, figureFileSmall=null, figureFileBig=null, tableContent=

基因	序列
miR-203 mimics	正义：5'-GUGAAAUGUUUAGGACCACUAG-3'
	反义：5'-AGUGGGUCCUAAACAUUUCACUU-3'
miR-203 mimics NC	正义：5'-UUCUCCGAACGUGUCACGUTT-3'
	反义：5'-ACGUGACACGUUCGGAGAATT-3'
miR-203 inhibitor	5'-CUAGUGGUCCUAAACAUUUCAC-3'
miR-203 inhibitor	NC 5'-CAGUACUUUUGUGUAGUACAA-3'

), ArticleFig(id=1207394346928804064, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=EN, label=Tab. 2, caption=

Primer sequence of qRT-PCR

, figureFileSmall=null, figureFileBig=null, tableContent=

基因	上游引物(5'-3')	下游引物(5'-3')
miR-203	AACCTTGCTCGTGAAATGTTTAG	TATGCTTGTTCTCGTCTCTGTGTC
U6	CTCGCTTCGGCAGCACA	AACGCTTCACGAATTTGCGT
CUL2	GGTTACAACATACCAAATGGCA	TGCTGTCCTGAAGCTCTTTATA
18S	GGAGTATGGTTGCAAAGCTGA	ATCTGTCAATCCTGTCCGTGT

), ArticleFig(id=1207394347012690152, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1207394340456993762, language=CN, label=表2, caption=

qRT-PCR引物序列

, figureFileSmall=null, figureFileBig=null, tableContent=

基因	上游引物(5'-3')	下游引物(5'-3')
miR-203	AACCTTGCTCGTGAAATGTTTAG	TATGCTTGTTCTCGTCTCTGTGTC
U6	CTCGCTTCGGCAGCACA	AACGCTTCACGAATTTGCGT
CUL2	GGTTACAACATACCAAATGGCA	TGCTGTCCTGAAGCTCTTTATA
18S	GGAGTATGGTTGCAAAGCTGA	ATCTGTCAATCCTGTCCGTGT

)], attaches=null, journal=Journal(id=1146441329971666965, delFlag=0, nameCn=解放军医学杂志, nameEn=Medical Journal of Chinese People’s Liberation Army, nameHistory1=null, nameHistory2=null, issn=0577-7402, eissn=null, cn=11-1056/R, coden=null, periodic=0, language=CN, oaType=是, ccby=CC BY-NC-ND, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=6srot5PcoYX30Oa4xeTmeg==, journalPrice=null, startedYear=null, abbrevIsoEn=null, journalRemark=null, publicationField=null, createdTime=1751262512917, updatedTime=1761735725513, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=M, firstLetterEn=M, subjectCode=Life Sciences, subjectName=Life Sciences, subjectCodeEn=Life Sciences, subjectNameEn=null, picCn=6srot5PcoYX30Oa4xeTmeg==, picEn=ELwBh5xqrSTlIs7HmSNt2Q==, jcr=null, cjcr=null, exts=[JournalExt(id=1190369167564968109, language=CN, name=解放军医学杂志, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1761735725537, updatedTime=1761735725537, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=#, submissionEditorUrl=#, submissionReviewUrl=#, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1190369167615299758, language=EN, name=Medical Journal of Chinese People’s Liberation Army, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1761735725549, updatedTime=1761735725549, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=#, submissionEditorUrl=#, submissionReviewUrl=#, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1189873630562394117, websiteList=[Website(id=1189873845923287108, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1189873630562394117, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/jfjyxzz/CN, language=CN, createTime=1761617631655, createBy=18614031015, updateTime=1761622010471, updateBy=18614031015, name=解放军医学杂志-中文, tplId=1146099689490845704, title=解放军医学杂志, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1189924939378520839, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=articleTextType, value=kx, createTime=1761629813284, updateTime=1761629813284, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939353355012, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=banner, value=null, createTime=1761629813278, updateTime=1761629813278, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939399492362, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=grayFlag, value=0, createTime=1761629813289, updateTime=1761629813289, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939344966403, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=logo, value=https://castjournals.cast.org.cn/joweb/jfjyxzz/CN/file/pic?fileId=+zXjYVhun8ZOAA6+aKx2hw==, createTime=1761629813276, updateTime=1761629813276, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939412075276, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=minRunFlag, value=0, createTime=1761629813292, updateTime=1761629813292, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939374326534, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/jfjyxzz/CN/file/pic, createTime=1761629813283, updateTime=1761629813283, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939407880971, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=silenceFlag, value=0, createTime=1761629813291, updateTime=1761629813291, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939361743621, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1761629813280, updateTime=1761629813280, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939386909448, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=themeColor, value=null, createTime=1761629813286, updateTime=1761629813286, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939395298057, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=themeStyle, value=null, createTime=1761629813288, updateTime=1761629813288, creator=18614031015, updator=18614031015)]), Website(id=1189873846057504839, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1189873630562394117, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/jfjyxzz/EN, language=EN, createTime=1761617631687, createBy=18614031015, updateTime=1761622030030, updateBy=18614031015, name=解放军医学杂志-英文, tplId=1146101810881728533, title=Medical Journal of Chinese People’s Liberation Army, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1189924968168223505, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=articleTextType, value=kx, createTime=1761629820148, updateTime=1761629820148, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968147251982, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=banner, value=null, createTime=1761629820143, updateTime=1761629820143, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968185000724, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=grayFlag, value=0, createTime=1761629820152, updateTime=1761629820152, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968138863373, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=logo, value=https://castjournals.cast.org.cn/joweb/jfjyxzz/EN/file/pic?fileId=+zXjYVhun8ZOAA6+aKx2hw==, createTime=1761629820141, updateTime=1761629820141, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968197583638, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=minRunFlag, value=0, createTime=1761629820155, updateTime=1761629820155, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968159834896, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/jfjyxzz/EN/file/pic, createTime=1761629820146, updateTime=1761629820146, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968193389333, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=silenceFlag, value=0, createTime=1761629820154, updateTime=1761629820154, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968155640591, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1761629820145, updateTime=1761629820145, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968172417810, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=themeColor, value=null, createTime=1761629820149, updateTime=1761629820149, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968180806419, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=themeStyle, value=null, createTime=1761629820151, updateTime=1761629820151, creator=18614031015, updator=18614031015)])], journalTitle=解放军医学杂志, weixinUrl=null, journalUrl=http://zh.jfjyxzz.org.cn/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Medical Journal of Chinese People’s Liberation Army, journalPhotoCn=6srot5PcoYX30Oa4xeTmeg==, journalPhotoEn=ELwBh5xqrSTlIs7HmSNt2Q==, journalFirstLetter=M, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false), detailUrlCn=https://castjournals.cast.org.cn/joweb/jfjyxzz/CN/10.11855/j.issn.0577-7402.2022.10.0968, detailUrlEn=https://castjournals.cast.org.cn/joweb/jfjyxzz/EN/10.11855/j.issn.0577-7402.2022.10.0968, pdfUrlCn=https://castjournals.cast.org.cn/joweb/jfjyxzz/CN/PDF/10.11855/j.issn.0577-7402.2022.10.0968, pdfUrlEn=https://castjournals.cast.org.cn/joweb/jfjyxzz/EN/PDF/10.11855/j.issn.0577-7402.2022.10.0968, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, reference=null)