首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1199688713337930440, tenantId=1146029695717560320, journalId=1189873630562394117, issueId=1199688705905623579, articleNumber=null, orderNo=null, doi=10.11855/j.issn.0577-7402.0408.2023.0807, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1679328000000, receivedDateStr=2023-03-21, revisedDate=null, revisedDateStr=null, acceptedDate=1688400000000, acceptedDateStr=2023-07-04, onlineDate=1763957678398, onlineDateStr=2025-11-24, pubDate=1703692800000, pubDateStr=2023-12-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1763957678398, onlineIssueDateStr=2025-11-24, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1763957678398, creator=13701087609, updateTime=1763957678398, updator=13701087609, issue=Issue{id=1199688705905623579, tenantId=1146029695717560320, journalId=1189873630562394117, year='2023', volume='48', issue='12', pageStart='1359', pageEnd='1491', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=0, createTime=1763957676626, creator=13701087609, updateTime=1763958367038, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1199691601774739748, tenantId=1146029695717560320, journalId=1189873630562394117, issueId=1199688705905623579, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1199691601774739749, tenantId=1146029695717560320, journalId=1189873630562394117, issueId=1199688705905623579, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=1412, endPage=1419, ext={EN=ArticleExt(id=1199688713652503257, articleId=1199688713337930440, tenantId=1146029695717560320, journalId=1189873630562394117, language=EN, title=Primary study of heat stress inducing early expression and secretion of tissue factor in vascular endothelial cells, columnId=1190310110212751762, journalTitle=Medical Journal of Chinese People’s Liberation Army, columnName=Basic Research, runingTitle=null, highlight=null, articleAbstract=

Objective To explore the pattern of early expression and secretion of tissue factor (TF) in vascular endothelial cells induced by heat stress. Methods Thirty SPF-rated C57BL/6 male mice were randomly divided into five groups: the control group and groups of indicated recovery time, including 0, 3, 6, and 9 h in room temperature after heat stress (n=6). Mice in the heat stress groups were exposed to an animal incubator to reach 42.5 ℃ for core body temperature for heat stroke. We analyzed the histopathological changes in the liver, lung, and kidney tissues with HE staining. We measured the TF mRNA in mice tissues by RT-qPCR and the plasma concentration of TF in mice with a commercial ELISA kit. Human umbilical vein endothelial cells (HUVECs) were placed in a culture incubator to build an in vitro heat stress model. HUVECs were divided into five groups, including a control group and groups of indicated recovery time, including 0, 3, 6, and 9 h after heat stress. We quantified the expression of TF mRNA and protein in HUVEC cells by RT-qPCR, Western blotting, and immunofluorescence and measured the secreted TF with a commercial ELISA kit. Results No significant pathological injury was observed in the tissues of the control group. Mice treated with heat stress had various degrees of structural injuries and hemorrhagic and inflammatory changes in multiple tissues. Compared to control group, the expression of TF mRNA significantly increased in the kidney of heat stress-treated mice with 0 and 3 h recovery time (1.719±0.018, 1.241±0.178 vs. 1.000±0.063), the lung with 3 h recovery time (2.444±0.511 vs. 1.000±0.106) and the liver with 6 h recovery time (7.312±0.618 vs. 1.000±0.147) (P<0.05). The concentration of TF in plasma also sustainedly elevated in mice with 0, 3, 6, and 9 h recovery time after heat stress as compared to control group [(132.426±17.920) pg/ml, (119.400±10.267) pg/ml, (107.374±13.495) pg/ml, (163.767±22.810) pg/ml vs. (75.479±13.831) pg/ml, respectively, P<0.01]. The expression levels of TF mRNA were higher in heat stress HUVECs with 6 h and 9 h recovery time than the control cells (1.905±0.354, 2.564±0.297 vs. 1.000±0.097, P<0.01). Secreted TF in the supernatant from HUVECs treated with heat stress and different recovery time also increased significantly [(36.309±4.101) pg/ml, (38.425±5.484) pg/ml, (41.655±4.380) pg/ml, (43.586±4.718) pg/ml vs. (14.996±0.254) pg/ml, P<0.01]. Conclusion Heat stress increased early expression and secretion of TF in vascular endothelial cells. Vascular endothelial cells may be a main source of circulating TF in heat stroke.

, correspAuthors=Hua-Sheng Tong, Wei-Liang Zhou, authorNote=null, correspAuthorsNote=
Tong Hua-Sheng, E-mail:
Zhou Wei-Liang, E-mail:
, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Jing Qian, Fan-Fan Wang, Lu-Lu Wan, Jia-Le Yang, Xue-Zhi Shi, Hua-Sheng Tong, Wei-Liang Zhou), CN=ArticleExt(id=1199688715586077523, articleId=1199688713337930440, tenantId=1146029695717560320, journalId=1189873630562394117, language=CN, title=热打击诱导血管内皮细胞组织因子早期表达分泌的初步研究, columnId=1190310110472798614, journalTitle=解放军医学杂志, columnName=基础研究, runingTitle=null, highlight=null, articleAbstract=

目的 探讨热打击诱导血管内皮细胞组织因子(TF)早期表达分泌的规律。方法 将30只SPF级C57BL/6雄性小鼠随机分为对照组与热打击后复温0、3、6、9 h组(n=6)。热打击组小鼠暴露于动物孵箱热环境，核心温度达42.5 ℃时即为发生重症中暑。采用HE染色观察小鼠肝脏、肺脏及肾脏组织病理学变化；RT-qPCR检测小鼠组织TF mRNA表达水平；ELISA法检测小鼠血浆TF浓度。体外建立人脐静脉内皮细胞(HUVECs)热打击模型，分为对照组与热打击后复温0、3、6、9 h组。采用RT-qPCR、Western blotting及细胞免疫荧光化学技术分别检测HUVECs中TF mRNA及蛋白表达水平；采用ELISA法检测HUVECs培养上清中TF水平。结果 对照组小鼠各组织未见明显病理损伤，热打击复温小鼠存在不同程度组织细胞结构性损伤及出血性、炎症性损伤。与对照组小鼠比较，热打击复温0、3 h组肾脏(1.719±0.018、1.241±0.178 vs. 1.000±0.063)、复温3 h组肺脏(2.444±0.511 vs. 1.000±0.106)及复温6 h组肝脏(7.312±0.618 vs. 1.000±0.147)中TF mRNA表达量均明显升高(P<0.05)。热打击小鼠复温0、3、6、9 h组血浆TF水平均较对照组小鼠升高[(132.426±17.920) pg/ml、(119.400±10.267) pg/ml、(107.374±13.495) pg/ml、(163.767±22.810) pg/ml vs. (75.479±13.831) pg/ml，P<0.01]。与对照组比较，热打击后复温6、9 h组HUVECs的TF mRNA表达水平明显升高(1.905±0.354、2.564±0.297 vs. 1.000±0.097，P<0.01)；热打击复温0、3、6、9 h组HUVECs培养上清TF水平均明显高于对照组[(36.309±4.101) pg/ml、(38.425±5.484) pg/ml、(41.655±4.380) pg/ml、(43.586±4.718) pg/ml vs. (14.996±0.254) pg/ml，P<0.01]。结论 热打击血管内皮细胞中TF的早期表达分泌增加，可能是重症中暑循环TF的主要来源之一。

, correspAuthors=童华生, 周伟梁, authorNote=null, correspAuthorsNote=
周伟梁，E-mail：
童华生，E-mail：
, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=lkj9nbMF1cNEx28d3C6kkg==, magXml=qw2hyp9uu2XkOA1wrsg94Q==, pdfUrl=null, pdf=De4o2DxUDM/QnpYSRkP8uw==, pdfFileSize=6633233, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=2DoPKjXUgX1TO+4m3fsXGQ==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=t5nrhqA00WKmfVK4ppk7Eg==, mapNumber=null, authorCompany=null, fund=null, authors=

钱晶，硕士研究生，主要从事重症中暑弥散性血管内凝血发病机制方面的研究

, authorsList=钱晶, 王凡凡, 万露露, 杨加乐, 施学智, 童华生, 周伟梁)}, authors=[Author(id=1199700881642979419, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1199700881731059807, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700881642979419, language=EN, stringName=Jing Qian, firstName=Jing, middleName=null, lastName=Qian, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510000, China
2Department of Critical Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510000, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1199700881819140196, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700881642979419, language=CN, stringName=钱晶, firstName=晶, middleName=null, lastName=钱, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1广州中医药大学研究生院，广东广州 510000
2南部战区总医院重症医学科，广东广州 510000, bio={"content":"

钱晶，硕士研究生，主要从事重症中暑弥散性血管内凝血发病机制方面的研究

"}, bioImg=null, bioContent=

钱晶，硕士研究生，主要从事重症中暑弥散性血管内凝血发病机制方面的研究

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1199700880858644532, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=1, ext=[AuthorCompanyExt(id=1199700880867033140, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880858644532, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510000, China), AuthorCompanyExt(id=1199700880875421750, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880858644532, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1广州中医药大学研究生院，广东广州 510000)]), AuthorCompany(id=1199700880967696439, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=2, ext=[AuthorCompanyExt(id=1199700880976085049, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880967696439, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2Department of Critical Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510000, China), AuthorCompanyExt(id=1199700881026416701, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880967696439, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2南部战区总医院重症医学科，广东广州 510000)])]), Author(id=1199700881932386411, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1199700882037244013, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700881932386411, language=EN, stringName=Fan-Fan Wang, firstName=Fan-Fan, middleName=null, lastName=Wang, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=3, address=3The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510000, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1199700882133713010, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700881932386411, language=CN, stringName=王凡凡, firstName=凡凡, middleName=null, lastName=王, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=3, address=3南方医科大学第一临床医学院，广东广州 510000, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1199700881177411652, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=3, ext=[AuthorCompanyExt(id=1199700881181605957, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700881177411652, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510000, China), AuthorCompanyExt(id=1199700881189994566, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700881177411652, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3南方医科大学第一临床医学院，广东广州 510000)])]), Author(id=1199700882221793399, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1199700882330845305, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700882221793399, language=EN, stringName=Lu-Lu Wan, firstName=Lu-Lu, middleName=null, lastName=Wan, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=4, address=4Shenzhen Clinical School of Medicine, Guangzhou University of Chinese Medicine, Shenzhen, Guangdong 518000, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1199700882427314300, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700882221793399, language=CN, stringName=万露露, firstName=露露, middleName=null, lastName=万, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=4, address=4广州中医药大学深圳临床医学院，广东深圳 518000, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1199700881345183818, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=4, ext=[AuthorCompanyExt(id=1199700881357766731, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700881345183818, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=4Shenzhen Clinical School of Medicine, Guangzhou University of Chinese Medicine, Shenzhen, Guangdong 518000, China), AuthorCompanyExt(id=1199700881366155340, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700881345183818, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=4广州中医药大学深圳临床医学院，广东深圳 518000)])]), Author(id=1199700882502811776, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1199700882620252291, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700882502811776, language=EN, stringName=Jia-Le Yang, firstName=Jia-Le, middleName=null, lastName=Yang, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510000, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1199700882762858630, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700882502811776, language=CN, stringName=杨加乐, firstName=加乐, middleName=null, lastName=杨, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1广州中医药大学研究生院，广东广州 510000, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1199700880858644532, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=1, ext=[AuthorCompanyExt(id=1199700880867033140, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880858644532, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510000, China), AuthorCompanyExt(id=1199700880875421750, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880858644532, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1广州中医药大学研究生院，广东广州 510000)])]), Author(id=1199700883882737804, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1199700883991789711, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700883882737804, language=EN, stringName=Xue-Zhi Shi, firstName=Xue-Zhi, middleName=null, lastName=Shi, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2Department of Critical Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510000, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1199700884146978964, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700883882737804, language=CN, stringName=施学智, firstName=学智, middleName=null, lastName=施, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2南部战区总医院重症医学科，广东广州 510000, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1199700880967696439, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=2, ext=[AuthorCompanyExt(id=1199700880976085049, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880967696439, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2Department of Critical Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510000, China), AuthorCompanyExt(id=1199700881026416701, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880967696439, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2南部战区总医院重症医学科，广东广州 510000)])]), Author(id=1199700884235059352, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, orderNo=5, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=fimmuths@163.com, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1199700884369277086, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700884235059352, language=EN, stringName=Hua-Sheng Tong, firstName=Hua-Sheng, middleName=null, lastName=Tong, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, *, address=1Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510000, China
2Department of Critical Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510000, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1199700884482523296, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700884235059352, language=CN, stringName=童华生, firstName=华生, middleName=null, lastName=童, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, *, address=1广州中医药大学研究生院，广东广州 510000
2南部战区总医院重症医学科，广东广州 510000, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1199700880858644532, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=1, ext=[AuthorCompanyExt(id=1199700880867033140, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880858644532, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510000, China), AuthorCompanyExt(id=1199700880875421750, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880858644532, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1广州中医药大学研究生院，广东广州 510000)]), AuthorCompany(id=1199700880967696439, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=2, ext=[AuthorCompanyExt(id=1199700880976085049, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880967696439, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2Department of Critical Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510000, China), AuthorCompanyExt(id=1199700881026416701, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880967696439, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2南部战区总医院重症医学科，广东广州 510000)])]), Author(id=1199700884595769510, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, orderNo=6, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=710833932@qq.com, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1199700884763541674, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700884595769510, language=EN, stringName=Wei-Liang Zhou, firstName=Wei-Liang, middleName=null, lastName=Zhou, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=5, *, address=5Department of Emergency Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510000, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1199700884860010670, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, authorId=1199700884595769510, language=CN, stringName=周伟梁, firstName=伟梁, middleName=null, lastName=周, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=5, *, address=5南部战区总医院急诊医学科，广东广州 510000, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1199700881542316113, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=5, ext=[AuthorCompanyExt(id=1199700881546510419, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700881542316113, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=5Department of Emergency Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510000, China), AuthorCompanyExt(id=1199700881554899028, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700881542316113, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=5南部战区总医院急诊医学科，广东广州 510000)])])], keywords=[Keyword(id=1199700885048754356, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=EN, orderNo=1, keyword=heat stress), Keyword(id=1199700885153611961, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=EN, orderNo=2, keyword=vascular endothelial cells), Keyword(id=1199700885283635387, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=EN, orderNo=3, keyword=tissue factor), Keyword(id=1199700885346549949, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=EN, orderNo=4, keyword=expression and secretion), Keyword(id=1199700885459796162, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=CN, orderNo=1, keyword=热打击), Keyword(id=1199700885539487940, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=CN, orderNo=2, keyword=血管内皮细胞), Keyword(id=1199700885631762631, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=CN, orderNo=3, keyword=组织因子), Keyword(id=1199700885757591753, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=CN, orderNo=4, keyword=表达分泌)], refs=[Reference(id=1199700889435996433, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2021, volume=8, issue=3, pageStart=405, pageEnd=423, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=Edward Tom Ashworth, James David Cotter, Andrew Edward Kilding, journalName=Mil. Med. Res., refType=null, unstructuredReference=Edward Tom Ashworth, James David Cotter, Andrew Edward Kilding. Methods for improving thermal tolerance in military personnel prior to deployment[J]. Mil. Med. Res., 2021, 8(3): 405-423., articleTitle=Methods for improving thermal tolerance in military personnel prior to deployment, refAbstract=null), Reference(id=1199700889574408468, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2020, volume=45, issue=3, pageStart=292, pageEnd=297, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=Yu BJ, Wang L, Peng N, journalName=Med J Chin PLA, refType=null, unstructuredReference=Yu BJ, Wang L, Peng N, et al. Study on the correlation of HMGB1 and coagulation disorder in severe heatstroke rats[J]. Med J Chin PLA, 2020, 45(3): 292-297., articleTitle=Study on the correlation of HMGB1 and coagulation disorder in severe heatstroke rats, refAbstract=null), Reference(id=1199700889670877464, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2020, volume=45, issue=3, pageStart=292, pageEnd=297, url=null, language=null, rfNumber=[2], rfOrder=2, authorNames=余保军, 王黎, 彭娜, journalName=解放军医学杂志, refType=null, unstructuredReference=余保军, 王黎, 彭娜, 等. HMGB1与重症中暑大鼠凝血紊乱的相关性分析[J]. 解放军医学杂志, 2020, 45(3): 292-297., articleTitle=HMGB1与重症中暑大鼠凝血紊乱的相关性分析, refAbstract=null), Reference(id=1199700889905758493, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2012, volume=40, issue=4, pageStart=1370, pageEnd=1372, url=null, language=null, rfNumber=[3], rfOrder=3, authorNames=Jilma B, Derhaschnig U, journalName=Crit Care Med, refType=null, unstructuredReference=Jilma B, Derhaschnig U. Disseminated intravascular coagulation in heat stroke: a hot topic[J]. Crit Care Med, 2012, 40(4): 1370-1372., articleTitle=Disseminated intravascular coagulation in heat stroke: a hot topic, refAbstract=null), Reference(id=1199700890019004703, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2012, volume=40, issue=4, pageStart=1229, pageEnd=1236, url=null, language=null, rfNumber=[4], rfOrder=4, authorNames=Bouchama A, Al-Mohanna F, Assad L, journalName=Crit Care Med, refType=null, unstructuredReference=Bouchama A, Al-Mohanna F, Assad L, et al. Tissue factor/factor Ⅶa pathway mediates coagulation activation in induced-heat stroke in the baboon[J]. Crit Care Med, 2012, 40(4): 1229-1236., articleTitle=Tissue factor/factor Ⅶa pathway mediates coagulation activation in induced-heat stroke in the baboon, refAbstract=null), Reference(id=1199700890199359781, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=1985, volume=61, issue=3, pageStart=517, pageEnd=523, url=null, language=null, rfNumber=[5], rfOrder=5, authorNames=Mustafa KY, Omer O, Khogali M, journalName=Br J Haematol, refType=null, unstructuredReference=Mustafa KY, Omer O, Khogali M, et al. Blood coagulation and fibrinolysis in heat stroke[J]. Br J Haematol, 1985, 61(3): 517-523., articleTitle=Blood coagulation and fibrinolysis in heat stroke, refAbstract=null), Reference(id=1199700890333577511, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2022, volume=44, issue=null, pageStart=101276, pageEnd=null, url=null, language=null, rfNumber=[6], rfOrder=6, authorNames=Iba T, Connors JM, Levi M, journalName=EClinicalMedicine, refType=null, unstructuredReference=Iba T, Connors JM, Levi M, et al. Heatstroke-induced coagulopathy: Biomarkers, mechanistic insights, and patient management[J]. EClinicalMedicine, 2022, 44: 101276., articleTitle=Heatstroke-induced coagulopathy: Biomarkers, mechanistic insights, and patient management, refAbstract=null), Reference(id=1199700890413269292, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2014, volume=62, issue=2, pageStart=57, pageEnd=62, url=null, language=null, rfNumber=[7], rfOrder=7, authorNames=Bode M, Mackman N, journalName=Vascul Pharmacol, refType=null, unstructuredReference=Bode M, Mackman N. Regulation of tissue factor gene expression in monocytes and endothelial cells: Thromboxane A2 as a new player[J]. Vascul Pharmacol, 2014, 62(2): 57-62., articleTitle=Regulation of tissue factor gene expression in monocytes and endothelial cells: Thromboxane A2 as a new player, refAbstract=null), Reference(id=1199700890518126895, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2010, volume=20, issue=6, pageStart=199, pageEnd=203, url=null, language=null, rfNumber=[8], rfOrder=8, authorNames=Eisenreich A, Rauch U, journalName=Trends Cardiovasc Med, refType=null, unstructuredReference=Eisenreich A, Rauch U. Regulation and differential role of the tissue factor isoforms in cardiovascular biology[J]. Trends Cardiovasc Med, 2010, 20(6): 199-203., articleTitle=Regulation and differential role of the tissue factor isoforms in cardiovascular biology, refAbstract=null), Reference(id=1199700890681704754, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=1996, volume=383, issue=6595, pageStart=73, pageEnd=75, url=null, language=null, rfNumber=[9], rfOrder=9, authorNames=Carmeliet P, Mackman N, Moons L, journalName=Nature, refType=null, unstructuredReference=Carmeliet P, Mackman N, Moons L, et al. Role of tissue factor in embryonic blood vessel development[J]. Nature, 1996, 383(6595): 73-75., articleTitle=Role of tissue factor in embryonic blood vessel development, refAbstract=null), Reference(id=1199700890799145268, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2016, volume=26, issue=4, pageStart=297, pageEnd=303, url=null, language=null, rfNumber=[10], rfOrder=10, authorNames=Witkowski M, Landmesser U, Rauch U, journalName=Trends Cardiovasc Med, refType=null, unstructuredReference=Witkowski M, Landmesser U, Rauch U. Tissue factor as a link between inflammation and coagulation[J]. Trends Cardiovasc Med, 2016, 26(4): 297-303., articleTitle=Tissue factor as a link between inflammation and coagulation, refAbstract=null), Reference(id=1199700890883031352, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2016, volume=26, issue=4, pageStart=304, pageEnd=305, url=null, language=null, rfNumber=[11], rfOrder=11, authorNames=Antoniak S, Mackman N, journalName=Trends Cardiovasc Med, refType=null, unstructuredReference=Antoniak S, Mackman N. Editorial Commentary: tissue factor expression by the endothelium: coagulation or inflammation?[J]. Trends Cardiovasc Med, 2016, 26(4): 304-305., articleTitle=Editorial Commentary: tissue factor expression by the endothelium: coagulation or inflammation?, refAbstract=null), Reference(id=1199700890945945915, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2006, volume=32, issue=1, pageStart=33, pageEnd=39, url=null, language=null, rfNumber=[12], rfOrder=12, authorNames=Levi M, van der Poll T, ten Cate H, journalName=Semin Thromb Hemost, refType=null, unstructuredReference=Levi M, van der Poll T, ten Cate H. Tissue factor in infection and severe inflammation[J]. Semin Thromb Hemost, 2006, 32(1): 33-39., articleTitle=Tissue factor in infection and severe inflammation, refAbstract=null), Reference(id=1199700891021443390, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2018, volume=16, issue=10, pageStart=1941, pageEnd=1952, url=null, language=null, rfNumber=[13], rfOrder=13, authorNames=Zelaya H, Rothmeier AS, Ruf W, journalName=J Thromb Haemost JTH, refType=null, unstructuredReference=Zelaya H, Rothmeier AS, Ruf W. Tissue factor at the crossroad of coagulation and cell signaling[J]. J Thromb Haemost JTH, 2018, 16(10): 1941-1952., articleTitle=Tissue factor at the crossroad of coagulation and cell signaling, refAbstract=null), Reference(id=1199700891147272514, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=1996, volume=24, issue=7, pageStart=1173, pageEnd=1178, url=null, language=null, rfNumber=[14], rfOrder=14, authorNames=Bouchama A, Hammami MM, Haq A, journalName=Crit Care Med, refType=null, unstructuredReference=Bouchama A, Hammami MM, Haq A, et al. Evidence for endothelial cell activation/injury in heatstroke[J]. Crit Care Med, 1996, 24(7): 1173-1178., articleTitle=Evidence for endothelial cell activation/injury in heatstroke, refAbstract=null), Reference(id=1199700891226964292, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2020, volume=45, issue=3, pageStart=270, pageEnd=276, url=null, language=null, rfNumber=[15], rfOrder=15, authorNames=Chen HS, Zhang M, Liang YX, journalName=Med J Chin PLA, refType=null, unstructuredReference=Chen HS, Zhang M, Liang YX, et al. Features and function of exosomes secreted by heat-stroke vascular endothelial cells[J]. Med J Chin PLA, 2020, 45(3): 270-276., articleTitle=Features and function of exosomes secreted by heat-stroke vascular endothelial cells, refAbstract=null), Reference(id=1199700891310850374, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2020, volume=45, issue=3, pageStart=270, pageEnd=276, url=null, language=null, rfNumber=[15], rfOrder=16, authorNames=陈怀生, 张明, 梁泳欣, journalName=解放军医学杂志, refType=null, unstructuredReference=陈怀生, 张明, 梁泳欣, 等. 热打击诱导血管内皮细胞释放的外泌体特征及其功能分析[J]. 解放军医学杂志, 2020, 45(3): 270-276., articleTitle=热打击诱导血管内皮细胞释放的外泌体特征及其功能分析, refAbstract=null), Reference(id=1199700891424096585, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2008, volume=36, issue=8, pageStart=2455, pageEnd=2456, url=null, language=null, rfNumber=[16], rfOrder=17, authorNames=Levi M, journalName=Crit Care Med, refType=null, unstructuredReference=Levi M. Burning issues surrounding inflammation and coagulation in heatstroke[J]. Crit Care Med, 2008, 36(8): 2455-2456., articleTitle=Burning issues surrounding inflammation and coagulation in heatstroke, refAbstract=null), Reference(id=1199700891533148492, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2022, volume=170, issue=null, pageStart=105653, pageEnd=null, url=null, language=null, rfNumber=[17], rfOrder=18, authorNames=Li Y, Li H, Tong HS, journalName=Microb Pathog, refType=null, unstructuredReference=Li Y, Li H, Tong HS, et al. Outer membrane vesicles derived from heatstroke-associated intestinal microbiota promote multiple organ injury in mice[J]. Microb Pathog, 2022, 170: 105653., articleTitle=Outer membrane vesicles derived from heatstroke-associated intestinal microbiota promote multiple organ injury in mice, refAbstract=null), Reference(id=1199700891638006095, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2011, volume=2011, issue=8, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[18], rfOrder=19, authorNames=Link AJ, LaBaer J, journalName=Cold Spring Harb Protoc, refType=null, unstructuredReference=Link AJ, LaBaer J. Trichloroacetic acid (TCA) precipitation of proteins[J]. Cold Spring Harb Protoc, 2011, 2011(8): pdb.prot5651., articleTitle=Trichloroacetic acid (TCA) precipitation of proteins, refAbstract=null), Reference(id=1199700892774662483, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2008, volume=28, issue=6, pageStart=1130, pageEnd=1136, url=null, language=null, rfNumber=[19], rfOrder=20, authorNames=Roberts GT, Ghebeh H, Chishti MA, journalName=Arterioscler Thromb Vasc Biol, refType=null, unstructuredReference=Roberts GT, Ghebeh H, Chishti MA, et al. Microvascular injury, thrombosis, inflammation, and apoptosis in the pathogenesis of heatstroke: a study in baboon model[J]. Arterioscler Thromb Vasc Biol, 2008, 28(6): 1130-1136., articleTitle=Microvascular injury, thrombosis, inflammation, and apoptosis in the pathogenesis of heatstroke: a study in baboon model, refAbstract=null), Reference(id=1199700892845965654, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2008, volume=36, issue=8, pageStart=2288, pageEnd=2295, url=null, language=null, rfNumber=[20], rfOrder=21, authorNames=Huisse MG, Pease S, Hurtado-Nedelec M, journalName=Crit Care Med, refType=null, unstructuredReference=Huisse MG, Pease S, Hurtado-Nedelec M, et al. Leukocyte activation: the link between inflammation and coagulation during heatstroke. A study of patients during the 2003 heat wave in Paris[J]. Crit Care Med, 2008, 36(8): 2288-2295., articleTitle=Leukocyte activation: the link between inflammation and coagulation during heatstroke. A study of patients during the 2003 heat wave in Paris, refAbstract=null), Reference(id=1199700892925657434, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2000, volume=7, issue=5, pageStart=273, pageEnd=277, url=null, language=null, rfNumber=[21], rfOrder=22, authorNames=Rauch U, Nemerson Y, journalName=Curr Opin Hematol, refType=null, unstructuredReference=Rauch U, Nemerson Y. Circulating tissue factor and thrombosis[J]. Curr Opin Hematol, 2000, 7(5): 273-277., articleTitle=Circulating tissue factor and thrombosis, refAbstract=null), Reference(id=1199700893022126428, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2000, volume=96, issue=2, pageStart=554, pageEnd=559, url=null, language=null, rfNumber=[22], rfOrder=23, authorNames=Franco RF, de Jonge E, Dekkers PE, journalName=Blood, refType=null, unstructuredReference=Franco RF, de Jonge E, Dekkers PE, et al. The in vivo kinetics of tissue factor messenger RNA expression during human endotoxemia: relationship with activation of coagulation[J]. Blood, 2000, 96(2): 554-559., articleTitle=The in vivo kinetics of tissue factor messenger RNA expression during human endotoxemia: relationship with activation of coagulation, refAbstract=null), Reference(id=1199700893097623902, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2018, volume=38, issue=4, pageStart=709, pageEnd=725, url=null, language=null, rfNumber=[23], rfOrder=24, authorNames=Grover SP, Mackman N, journalName=Arterioscler Thromb Vasc Biol, refType=null, unstructuredReference=Grover SP, Mackman N. Tissue factor[J]. Arterioscler Thromb Vasc Biol, 2018, 38(4): 709-725., articleTitle=Tissue factor, refAbstract=null), Reference(id=1199700893177315680, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=2005, volume=289, issue=2, pageStart=H873, pageEnd=H885, url=null, language=null, rfNumber=[24], rfOrder=25, authorNames=Wu SQ, Aird WC, journalName=Am J Physiol Heart Circ Physiol, refType=null, unstructuredReference=Wu SQ, Aird WC. Thrombin, TNF-α, and LPS exert overlapping but nonidentical effects on gene expression in endothelial cells and vascular smooth muscle cells[J]. Am J Physiol Heart Circ Physiol, 2005, 289(2): H873-H885., articleTitle=Thrombin, TNF-α, and LPS exert overlapping but nonidentical effects on gene expression in endothelial cells and vascular smooth muscle cells, refAbstract=null), Reference(id=1199700893290561890, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=1999, volume=19, issue=2, pageStart=281, pageEnd=289, url=null, language=null, rfNumber=[25], rfOrder=26, authorNames=Houston P, Dickson MC, Ludbrook V, journalName=Arterioscler Thromb Vasc Biol, refType=null, unstructuredReference=Houston P, Dickson MC, Ludbrook V, et al. Fluid shear stress induction of the tissue factor promoter in vitro and in vivo is mediated by Egr-1[J]. Arterioscler Thromb Vasc Biol, 1999, 19(2): 281-289., articleTitle=Fluid shear stress induction of the tissue factor promoter in vitro and in vivo is mediated by Egr-1, refAbstract=null), Reference(id=1199700893408002404, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=1995, volume=15, issue=5, pageStart=612, pageEnd=621, url=null, language=null, rfNumber=[26], rfOrder=27, authorNames=Parry GCN, Mackman N, journalName=Arterioscler Thromb Vasc Biol, refType=null, unstructuredReference=Parry GCN, Mackman N. Transcriptional regulation of tissue factor expression in human endothelial cells[J]. Arterioscler Thromb Vasc Biol, 1995, 15(5): 612-621., articleTitle=Transcriptional regulation of tissue factor expression in human endothelial cells, refAbstract=null), Reference(id=1199700893475111270, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=1990, volume=265, issue=17, pageStart=9782, pageEnd=9787, url=null, language=null, rfNumber=[27], rfOrder=28, authorNames=Crossman DC, Carr DP, Tuddenham EG, journalName=J Biol Chem, refType=null, unstructuredReference=Crossman DC, Carr DP, Tuddenham EG, et al. The regulation of tissue factor mRNA in human endothelial cells in response to endotoxin or phorbol ester[J]. J Biol Chem, 1990, 265(17): 9782-9787., articleTitle=The regulation of tissue factor mRNA in human endothelial cells in response to endotoxin or phorbol ester, refAbstract=null), Reference(id=1199700893554803048, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, doi=null, pmid=null, pmcid=null, year=1984, volume=160, issue=2, pageStart=618, pageEnd=623, url=null, language=null, rfNumber=[28], rfOrder=29, authorNames=Bevilacqua MP, Pober JS, Majeau GR, journalName=J Exp Med, refType=null, unstructuredReference=Bevilacqua MP, Pober JS, Majeau GR, et al. Interleukin 1 (IL-1) induces biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells[J]. J Exp Med, 1984, 160(2): 618-623., articleTitle=Interleukin 1 (IL-1) induces biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells, refAbstract=null)], funds=[Fund(id=1199700888781684999, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, awardId=2022A1515010353, language=EN, fundingSource=Natural Science Foundation of Guangdong Province(2022A1515010353), fundOrder=null, country=null), Fund(id=1199700888882348297, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, awardId=2022A1515010353, language=CN, fundingSource=广东省自然科学基金(2022A1515010353), fundOrder=null, country=null), Fund(id=1199700889062703372, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, awardId=18CXZ032, language=EN, fundingSource=Special Items of Military Medical Innovation Project(18CXZ032), fundOrder=null, country=null), Fund(id=1199700889180143885, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, awardId=18CXZ032, language=CN, fundingSource=军事医学创新工程专项项目(18CXZ032), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1199700880858644532, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=1, ext=[AuthorCompanyExt(id=1199700880867033140, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880858644532, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510000, China), AuthorCompanyExt(id=1199700880875421750, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880858644532, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1广州中医药大学研究生院，广东广州 510000)]), AuthorCompany(id=1199700880967696439, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=2, ext=[AuthorCompanyExt(id=1199700880976085049, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880967696439, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2Department of Critical Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510000, China), AuthorCompanyExt(id=1199700881026416701, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700880967696439, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2南部战区总医院重症医学科，广东广州 510000)]), AuthorCompany(id=1199700881177411652, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=3, ext=[AuthorCompanyExt(id=1199700881181605957, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700881177411652, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510000, China), AuthorCompanyExt(id=1199700881189994566, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700881177411652, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3南方医科大学第一临床医学院，广东广州 510000)]), AuthorCompany(id=1199700881345183818, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=4, ext=[AuthorCompanyExt(id=1199700881357766731, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700881345183818, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=4Shenzhen Clinical School of Medicine, Guangzhou University of Chinese Medicine, Shenzhen, Guangdong 518000, China), AuthorCompanyExt(id=1199700881366155340, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700881345183818, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=4广州中医药大学深圳临床医学院，广东深圳 518000)]), AuthorCompany(id=1199700881542316113, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, xref=5, ext=[AuthorCompanyExt(id=1199700881546510419, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700881542316113, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=5Department of Emergency Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510000, China), AuthorCompanyExt(id=1199700881554899028, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, companyId=1199700881542316113, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=5南部战区总医院急诊医学科，广东广州 510000)])], figs=[ArticleFig(id=1199700885996667087, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=EN, label=Fig.1, caption=Comparison of TF mRNA expression levels in kidney (A), lung (B), and liver (C) tissues of mice in each group (n=3), figureFileSmall=ba9gFg1w8IFc7vYCXI4EFw==, figureFileBig=9yeG0zXzH4tqFeUzM7LQuQ==, tableContent=null), ArticleFig(id=1199700886135079124, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=CN, label=图1, caption=各组小鼠肾(A)、肺(B)及肝(C)组织TF mRNA表达量比较(n=3)

TF. 组织因子；与对照组比较，(1)P<0.05

, figureFileSmall=ba9gFg1w8IFc7vYCXI4EFw==, figureFileBig=9yeG0zXzH4tqFeUzM7LQuQ==, tableContent=null), ArticleFig(id=1199700886294462681, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=EN, label=Fig.2, caption=Comparison of plasma TF content in each group of mice (n=6), figureFileSmall=hoIaqTu0ZmMrg0KBKoOekw==, figureFileBig=SLdHnSA/L+msz2FybFte/Q==, tableContent=null), ArticleFig(id=1199700886437069021, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=CN, label=图2, caption=各组小鼠血浆TF含量比较(n=6)

TF. 组织因子；与对照组比较，(1)P<0.01

, figureFileSmall=hoIaqTu0ZmMrg0KBKoOekw==, figureFileBig=SLdHnSA/L+msz2FybFte/Q==, tableContent=null), ArticleFig(id=1199700886541926624, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=EN, label=Fig.3, caption=Pathological observation of different organs and tissues in each group of mice (HE staining, the black arrow indicates the location of thrombosis), figureFileSmall=EAV8Z1fc9AlRMQSX4l6KsA==, figureFileBig=g5UVlPzKqX+ptQiiHcgU3A==, tableContent=null), ArticleFig(id=1199700886655172834, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=CN, label=图3, caption=各组小鼠不同器官组织病理学观察 (HE染色，黑色箭头所指为血栓形成处)

A. 肾；B. 肺；C. 肝

, figureFileSmall=EAV8Z1fc9AlRMQSX4l6KsA==, figureFileBig=g5UVlPzKqX+ptQiiHcgU3A==, tableContent=null), ArticleFig(id=1199700886764224743, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=EN, label=Fig.4, caption=Comparison of TF levels expressed and secreted by HUVECs in each group, figureFileSmall=Z2q7bEpZ90qD2VX04dNlHg==, figureFileBig=oOCYLCeyEA5MR9oK3VS79Q==, tableContent=null), ArticleFig(id=1199700886843916521, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=CN, label=图4, caption=各组HUVECs表达分泌TF水平比较

HUVECs. 人脐静脉内皮细胞；TF. 组织因子；GAPDH. 磷酸甘油醛脱氢酶；A. Western blotting检测HUVECs细胞培养上清及细胞内TF表达(n=3)；B. ELISA检测HUVECs培养上清中的TF含量(n=4)；与对照组比较，(1)P<0.01

, figureFileSmall=Z2q7bEpZ90qD2VX04dNlHg==, figureFileBig=oOCYLCeyEA5MR9oK3VS79Q==, tableContent=null), ArticleFig(id=1199700886927802605, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=EN, label=Fig.5, caption=Cellular immunofluorescence chemical detection of TF expression on HUVECs cytomembrane surface, figureFileSmall=YOReujoWnoGcsuY2hG3xHQ==, figureFileBig=eS5TxOUkVyTa2XXszijlXg==, tableContent=null), ArticleFig(id=1199700886994911473, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=CN, label=图5, caption=细胞免疫荧光化学检测HUVECs细胞膜表面TF表达

HUVECs. 人脐静脉内皮细胞；TF. 组织因子；A.细胞免疫荧光化学检测HUVECs细胞膜表面TF表达；蓝色为DAPI染核，绿色为细胞膜表面TF(n=3，×400)；B. HUVECs细胞膜表面TF相对荧光强度；与对照组比较，(1)P<0.01

, figureFileSmall=YOReujoWnoGcsuY2hG3xHQ==, figureFileBig=eS5TxOUkVyTa2XXszijlXg==, tableContent=null), ArticleFig(id=1199700887078797555, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=EN, label=Fig.6, caption=RT-qPCR detection of TF mRNA expression in HUVECs (n=3), figureFileSmall=cPdtnY/m4TzOrtQoAE3hew==, figureFileBig=UPhCz087O7toBwYd+nn1VQ==, tableContent=null), ArticleFig(id=1199700887162683638, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=CN, label=图6, caption=RT-qPCR检测HUVECs中TF mRNA表达(n=3)

HUVECs.人脐静脉内皮细胞；TF.组织因子；GAPDH.磷酸甘油醛脱氢酶；与对照组比较，(1)P<0.01

, figureFileSmall=cPdtnY/m4TzOrtQoAE3hew==, figureFileBig=UPhCz087O7toBwYd+nn1VQ==, tableContent=null), ArticleFig(id=1199700888295145722, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=EN, label=Tab.1, caption=

RT-qPCR Primer sequence for mouse tissues

, figureFileSmall=null, figureFileBig=null, tableContent=
引物名称引物序列
TF5'-CTGGAAAAACAAGTGCTTCTCG-3'
3'-ACAGAGAGGACCTTTGCTTCA-5'
GAPDH5'-AACGGATTTGGCCGTATTGG-3'
3'-CATTCTCGGCCTTGACTGTG-5'
), ArticleFig(id=1199700888420974844, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=CN, label=表1, caption=

小鼠组织RT-qPCR引物序列

, figureFileSmall=null, figureFileBig=null, tableContent=
引物名称引物序列
TF5'-CTGGAAAAACAAGTGCTTCTCG-3'
3'-ACAGAGAGGACCTTTGCTTCA-5'
GAPDH5'-AACGGATTTGGCCGTATTGG-3'
3'-CATTCTCGGCCTTGACTGTG-5'
), ArticleFig(id=1199700888504860927, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=EN, label=Tab.2, caption=

Human cellular RT-qPCR Primer sequence

, figureFileSmall=null, figureFileBig=null, tableContent=
引物名称引物序列
TF5'-GGCGCTTCAGGCACTACAA-3'
3'-TTGATTGACGGGTTTGGGTTC-5'
GAPDH5'-ACAACTTTGGTATCGTGGAAGG-3'
3'-GCCATCACGCCACAGTTTC-5'
), ArticleFig(id=1199700888605524226, tenantId=1146029695717560320, journalId=1189873630562394117, articleId=1199688713337930440, language=CN, label=表2, caption=

人类细胞RT-qPCR引物序列

, figureFileSmall=null, figureFileBig=null, tableContent=
引物名称引物序列
TF5'-GGCGCTTCAGGCACTACAA-3'
3'-TTGATTGACGGGTTTGGGTTC-5'
GAPDH5'-ACAACTTTGGTATCGTGGAAGG-3'
3'-GCCATCACGCCACAGTTTC-5'
)], attaches=null, journal=Journal(id=1146441329971666965, delFlag=0, nameCn=解放军医学杂志, nameEn=Medical Journal of Chinese People’s Liberation Army, nameHistory1=null, nameHistory2=null, issn=0577-7402, eissn=null, cn=11-1056/R, coden=null, periodic=0, language=CN, oaType=是, ccby=CC BY-NC-ND, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=6srot5PcoYX30Oa4xeTmeg==, journalPrice=null, startedYear=null, abbrevIsoEn=null, journalRemark=null, publicationField=null, createdTime=1751262512917, updatedTime=1761735725513, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=M, firstLetterEn=M, subjectCode=Life Sciences, subjectName=Life Sciences, subjectCodeEn=Life Sciences, subjectNameEn=null, picCn=6srot5PcoYX30Oa4xeTmeg==, picEn=ELwBh5xqrSTlIs7HmSNt2Q==, jcr=null, cjcr=null, exts=[JournalExt(id=1190369167564968109, language=CN, name=解放军医学杂志, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1761735725537, updatedTime=1761735725537, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=#, submissionEditorUrl=#, submissionReviewUrl=#, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1190369167615299758, language=EN, name=Medical Journal of Chinese People’s Liberation Army, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1761735725549, updatedTime=1761735725549, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=#, submissionEditorUrl=#, submissionReviewUrl=#, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1189873630562394117, websiteList=[Website(id=1189873845923287108, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1189873630562394117, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/jfjyxzz/CN, language=CN, createTime=1761617631655, createBy=18614031015, updateTime=1761622010471, updateBy=18614031015, name=解放军医学杂志-中文, tplId=1146099689490845704, title=解放军医学杂志, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1189924939378520839, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=articleTextType, value=kx, createTime=1761629813284, updateTime=1761629813284, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939353355012, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=banner, value=null, createTime=1761629813278, updateTime=1761629813278, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939399492362, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=grayFlag, value=0, createTime=1761629813289, updateTime=1761629813289, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939344966403, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=logo, value=https://castjournals.cast.org.cn/joweb/jfjyxzz/CN/file/pic?fileId=+zXjYVhun8ZOAA6+aKx2hw==, createTime=1761629813276, updateTime=1761629813276, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939412075276, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=minRunFlag, value=0, createTime=1761629813292, updateTime=1761629813292, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939374326534, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/jfjyxzz/CN/file/pic, createTime=1761629813283, updateTime=1761629813283, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939407880971, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=silenceFlag, value=0, createTime=1761629813291, updateTime=1761629813291, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939361743621, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1761629813280, updateTime=1761629813280, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939386909448, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=themeColor, value=null, createTime=1761629813286, updateTime=1761629813286, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924939395298057, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873845923287108, code=themeStyle, value=null, createTime=1761629813288, updateTime=1761629813288, creator=18614031015, updator=18614031015)]), Website(id=1189873846057504839, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1189873630562394117, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/jfjyxzz/EN, language=EN, createTime=1761617631687, createBy=18614031015, updateTime=1761622030030, updateBy=18614031015, name=解放军医学杂志-英文, tplId=1146101810881728533, title=Medical Journal of Chinese People’s Liberation Army, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1189924968168223505, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=articleTextType, value=kx, createTime=1761629820148, updateTime=1761629820148, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968147251982, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=banner, value=null, createTime=1761629820143, updateTime=1761629820143, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968185000724, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=grayFlag, value=0, createTime=1761629820152, updateTime=1761629820152, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968138863373, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=logo, value=https://castjournals.cast.org.cn/joweb/jfjyxzz/EN/file/pic?fileId=+zXjYVhun8ZOAA6+aKx2hw==, createTime=1761629820141, updateTime=1761629820141, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968197583638, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=minRunFlag, value=0, createTime=1761629820155, updateTime=1761629820155, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968159834896, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/jfjyxzz/EN/file/pic, createTime=1761629820146, updateTime=1761629820146, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968193389333, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=silenceFlag, value=0, createTime=1761629820154, updateTime=1761629820154, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968155640591, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1761629820145, updateTime=1761629820145, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968172417810, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=themeColor, value=null, createTime=1761629820149, updateTime=1761629820149, creator=18614031015, updator=18614031015), WebsiteProps(id=1189924968180806419, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189873846057504839, code=themeStyle, value=null, createTime=1761629820151, updateTime=1761629820151, creator=18614031015, updator=18614031015)])], journalTitle=解放军医学杂志, weixinUrl=null, journalUrl=http://zh.jfjyxzz.org.cn/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Medical Journal of Chinese People’s Liberation Army, journalPhotoCn=6srot5PcoYX30Oa4xeTmeg==, journalPhotoEn=ELwBh5xqrSTlIs7HmSNt2Q==, journalFirstLetter=M, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false), detailUrlCn=https://castjournals.cast.org.cn/joweb/jfjyxzz/CN/10.11855/j.issn.0577-7402.0408.2023.0807, detailUrlEn=https://castjournals.cast.org.cn/joweb/jfjyxzz/EN/10.11855/j.issn.0577-7402.0408.2023.0807, pdfUrlCn=https://castjournals.cast.org.cn/joweb/jfjyxzz/CN/PDF/10.11855/j.issn.0577-7402.0408.2023.0807, pdfUrlEn=https://castjournals.cast.org.cn/joweb/jfjyxzz/EN/PDF/10.11855/j.issn.0577-7402.0408.2023.0807, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, reference=null)
收藏切换
热打击诱导血管内皮细胞组织因子早期表达分泌的初步研究
收藏切换
PDF下载
钱晶 1, 2 , 王凡凡 3 , 万露露 4 , 杨加乐 1 , 施学智 2 , 童华生 1, 2, * , 周伟梁 5, *
解放军医学杂志 | 基础研究 2023,48(12): 1412-1419
收起
收藏切换
解放军医学杂志 | 基础研究 2023, 48(12): 1412-1419
热打击诱导血管内皮细胞组织因子早期表达分泌的初步研究
全屏
钱晶1, 2, 王凡凡3, 万露露4, 杨加乐1, 施学智2, 童华生1, 2, * , 周伟梁5, *
作者信息
  • 1广州中医药大学研究生院，广东广州 510000
  • 2南部战区总医院重症医学科，广东广州 510000
  • 3南方医科大学第一临床医学院，广东广州 510000
  • 4广州中医药大学深圳临床医学院，广东深圳 518000
  • 5南部战区总医院急诊医学科，广东广州 510000

    • 钱晶，硕士研究生，主要从事重症中暑弥散性血管内凝血发病机制方面的研究

通讯作者:

周伟梁，E-mail：
童华生，E-mail：
Primary study of heat stress inducing early expression and secretion of tissue factor in vascular endothelial cells
Jing Qian1, 2, Fan-Fan Wang3, Lu-Lu Wan4, Jia-Le Yang1, Xue-Zhi Shi2, Hua-Sheng Tong1, 2, * , Wei-Liang Zhou5, *
Affiliations
  • 1Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510000, China
  • 2Department of Critical Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510000, China
  • 3The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510000, China
  • 4Shenzhen Clinical School of Medicine, Guangzhou University of Chinese Medicine, Shenzhen, Guangdong 518000, China
  • 5Department of Emergency Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510000, China
出版时间: 2023-12-28 doi: 10.11855/j.issn.0577-7402.0408.2023.0807
文章导航
收藏切换
摘要
收起

目的 探讨热打击诱导血管内皮细胞组织因子(TF)早期表达分泌的规律。方法 将30只SPF级C57BL/6雄性小鼠随机分为对照组与热打击后复温0、3、6、9 h组(n=6)。热打击组小鼠暴露于动物孵箱热环境，核心温度达42.5 ℃时即为发生重症中暑。采用HE染色观察小鼠肝脏、肺脏及肾脏组织病理学变化；RT-qPCR检测小鼠组织TF mRNA表达水平；ELISA法检测小鼠血浆TF浓度。体外建立人脐静脉内皮细胞(HUVECs)热打击模型，分为对照组与热打击后复温0、3、6、9 h组。采用RT-qPCR、Western blotting及细胞免疫荧光化学技术分别检测HUVECs中TF mRNA及蛋白表达水平；采用ELISA法检测HUVECs培养上清中TF水平。结果 对照组小鼠各组织未见明显病理损伤，热打击复温小鼠存在不同程度组织细胞结构性损伤及出血性、炎症性损伤。与对照组小鼠比较，热打击复温0、3 h组肾脏(1.719±0.018、1.241±0.178 vs. 1.000±0.063)、复温3 h组肺脏(2.444±0.511 vs. 1.000±0.106)及复温6 h组肝脏(7.312±0.618 vs. 1.000±0.147)中TF mRNA表达量均明显升高(P<0.05)。热打击小鼠复温0、3、6、9 h组血浆TF水平均较对照组小鼠升高[(132.426±17.920) pg/ml、(119.400±10.267) pg/ml、(107.374±13.495) pg/ml、(163.767±22.810) pg/ml vs. (75.479±13.831) pg/ml，P<0.01]。与对照组比较，热打击后复温6、9 h组HUVECs的TF mRNA表达水平明显升高(1.905±0.354、2.564±0.297 vs. 1.000±0.097，P<0.01)；热打击复温0、3、6、9 h组HUVECs培养上清TF水平均明显高于对照组[(36.309±4.101) pg/ml、(38.425±5.484) pg/ml、(41.655±4.380) pg/ml、(43.586±4.718) pg/ml vs. (14.996±0.254) pg/ml，P<0.01]。结论 热打击血管内皮细胞中TF的早期表达分泌增加，可能是重症中暑循环TF的主要来源之一。

热打击  /  血管内皮细胞  /  组织因子  /  表达分泌

Objective To explore the pattern of early expression and secretion of tissue factor (TF) in vascular endothelial cells induced by heat stress. Methods Thirty SPF-rated C57BL/6 male mice were randomly divided into five groups: the control group and groups of indicated recovery time, including 0, 3, 6, and 9 h in room temperature after heat stress (n=6). Mice in the heat stress groups were exposed to an animal incubator to reach 42.5 ℃ for core body temperature for heat stroke. We analyzed the histopathological changes in the liver, lung, and kidney tissues with HE staining. We measured the TF mRNA in mice tissues by RT-qPCR and the plasma concentration of TF in mice with a commercial ELISA kit. Human umbilical vein endothelial cells (HUVECs) were placed in a culture incubator to build an in vitro heat stress model. HUVECs were divided into five groups, including a control group and groups of indicated recovery time, including 0, 3, 6, and 9 h after heat stress. We quantified the expression of TF mRNA and protein in HUVEC cells by RT-qPCR, Western blotting, and immunofluorescence and measured the secreted TF with a commercial ELISA kit. Results No significant pathological injury was observed in the tissues of the control group. Mice treated with heat stress had various degrees of structural injuries and hemorrhagic and inflammatory changes in multiple tissues. Compared to control group, the expression of TF mRNA significantly increased in the kidney of heat stress-treated mice with 0 and 3 h recovery time (1.719±0.018, 1.241±0.178 vs. 1.000±0.063), the lung with 3 h recovery time (2.444±0.511 vs. 1.000±0.106) and the liver with 6 h recovery time (7.312±0.618 vs. 1.000±0.147) (P<0.05). The concentration of TF in plasma also sustainedly elevated in mice with 0, 3, 6, and 9 h recovery time after heat stress as compared to control group [(132.426±17.920) pg/ml, (119.400±10.267) pg/ml, (107.374±13.495) pg/ml, (163.767±22.810) pg/ml vs. (75.479±13.831) pg/ml, respectively, P<0.01]. The expression levels of TF mRNA were higher in heat stress HUVECs with 6 h and 9 h recovery time than the control cells (1.905±0.354, 2.564±0.297 vs. 1.000±0.097, P<0.01). Secreted TF in the supernatant from HUVECs treated with heat stress and different recovery time also increased significantly [(36.309±4.101) pg/ml, (38.425±5.484) pg/ml, (41.655±4.380) pg/ml, (43.586±4.718) pg/ml vs. (14.996±0.254) pg/ml, P<0.01]. Conclusion Heat stress increased early expression and secretion of TF in vascular endothelial cells. Vascular endothelial cells may be a main source of circulating TF in heat stroke.

heat stress  /  vascular endothelial cells  /  tissue factor  /  expression and secretion
钱晶, 王凡凡, 万露露, 杨加乐, 施学智, 童华生, 周伟梁. 热打击诱导血管内皮细胞组织因子早期表达分泌的初步研究. 解放军医学杂志, 2023 , 48 (12) : 1412 -1419 . DOI: 10.11855/j.issn.0577-7402.0408.2023.0807
Jing Qian, Fan-Fan Wang, Lu-Lu Wan, Jia-Le Yang, Xue-Zhi Shi, Hua-Sheng Tong, Wei-Liang Zhou. Primary study of heat stress inducing early expression and secretion of tissue factor in vascular endothelial cells[J]. Medical Journal of Chinese People’s Liberation Army, 2023 , 48 (12) : 1412 -1419 . DOI: 10.11855/j.issn.0577-7402.0408.2023.0807
正文
收起
近年来，全球极端气候事件频发，高温日数增加，中暑的发病率随之增长。重症中暑伴高热、器官衰竭或严重代谢紊乱等常危及患者生命[1]，即使接受重症监护治疗，患者病死率仍居高不下。尸检结果显示，重症中暑死亡患者的多个器官均有广泛血栓形成，啮齿类及狒狒重症中暑动物模型体内凝血激活，导致弥散性血管内凝血(disseminated intravascular coagulation，DIC)及组织损伤[2-3]。即使治疗及时，多数重症中暑患者早期仍会出现多器官功能障碍，使长期残疾及死亡的风险上升。重症中暑多器官损伤由组织直接热损伤、凝血功能障碍及多因素诱发的全身炎症反应综合征(systemic inflammatory response syndrome，SIRS)发展而来[4]，其器官损伤机制尚不完全清楚，凝血激活甚至DIC可能是重症中暑多器官损伤发生的关键机制，并与高病死率有关[4-6]。
重症中暑凝血激活的启动机制目前仍不清楚。组织因子(tissue factor，TF)又名凝血因子Ⅲ(F3)，是一种细胞膜跨膜糖蛋白，也是凝血酶生成的主要启动因子[7]。TF由多种细胞分泌至血液循环中可启动凝血激活[8]，在DIC的发生中发挥核心作用[9]。正常情况下，血管内皮细胞低水平表达TF，受不同刺激后可诱导TF表达增多[8]。Witkowski等[10]提出，血管内皮细胞表达分泌TF在凝血及炎症反应中发挥了重要作用。严重感染及炎症释放的炎性因子诱导内皮细胞生成TF，亦有体外实验佐证，TF介导凝血酶产生，使纤维蛋白原转化为纤维蛋白，同时激活血小板释放P-选择素，促进TF进一步释放[11]。此外，TF与凝血酶结合并激活不同细胞的蛋白酶激活受体(protease-activated receptors，PARs)，诱导更多细胞因子表达[12]。血管内皮细胞TF参与炎症反应主要经细胞信号转导途径，而非激活凝血[13]。近年来，TF在炎症及凝血反应中的联结枢纽功能日益得到研究者们的重视。
临床病例分析及动物疾病模型显示，重症中暑宿主体内TF上升，验证了TF对重症中暑诱发凝血反应具有重要作用[3]。重症中暑患者体内的血管内皮损伤标志物血管性血友病因子(von Willebrand factor，vWF)及内皮素增加，表明存在内皮损伤[14-15]，但现有研究成果无法明确重症中暑对血管内皮细胞本身的影响。TF是炎症与凝血反应的重要联结，在被激活的单核细胞及受损的血管内皮细胞表面均有表达，但循环TF的具体细胞来源尚不清楚[16]。重症中暑作为一种剧烈的炎症及凝血反应状态，TF的表达分泌是否源自血管内皮细胞更是未知。本研究通过建立体内外热打击模型，初步探讨热打击血管内皮细胞TF早期表达分泌的规律。
1 材料与方法
收起
1.1 仪器与试剂
DMEM高糖培养基、胎牛血清、CO2培养箱(美国赛默飞世尔科技公司)，细胞培养双抗溶液(青霉素-链霉素溶液，美国HyClone公司)；BCA蛋白浓度测定试剂盒、彩色预染蛋白Marker(中国白鲨公司)。12.5%聚丙烯酰胺凝胶快速制备试剂盒(上海雅酶生物科技有限公司)，兔TF单抗(武汉爱博泰克生物科技有限公司，A4395)、兔TF多抗(北京博奥森生物科技有限公司，bs-4690R)、山羊抗兔IgG DyLight® 488(ab96899)及山羊抗兔IgG(ab97080)购自英国Abcam公司，GAPDH单克隆抗体(美国Proteintech公司，60004-1-Ig)，山羊抗小鼠IgG(北京中杉金桥生物技术有限公司，ZB-2305)。含DAPI抗荧光衰减封片剂(北京索莱宝科技有限公司)。去除基因组DNA实时荧光定量PCR(RT-qPCR)专用反转录试剂(日本TaKaRa公司，RR047A)，SYBR Green Pro Taq HS预混型qPCR试剂盒(湖南艾科瑞生物工程有限公司，AG11701)，Rotor-Gene RT-qPCR仪(德国Qiagen公司)。人TF酶联免疫吸附试验(ELISA)试剂盒、小鼠TF ELISA试剂盒(武汉贝茵莱生物科技有限公司)。三气培养箱(上海力新仪器有限公司)，动物专用培养箱(宁波戴维医疗器械有限公司)，动物专用体温计(中国康宁公司)；光学显微镜(日本Olympus公司)，荧光倒置显微镜(德国Leica公司)，台式离心机(日本Kubota公司)，高速冷冻离心机(德国Eppendorf公司)。
1.2 动物实验
1.2.1 实验动物
30只8~12周龄SPF级雄性C57BL/6小鼠(体重20~25 g)购自广州南方医大实验动物科技发展有限公司[动物合格证编号：SCXK(粤)2021-0041]。实验前动物自由进食进水，饲养于安静、恒温、恒湿的洁净环境，12 h光照及12 h黑暗交替变化，使用动物专用测温计测量核心体温。本实验获南部战区总医院实验动物伦理委员会审核批准(动物伦理审查编号：2021111801)，实验过程符合国家及单位有关实验动物的管理及使用规定。
1.2.2 热打击小鼠模型建立
根据随机数字表法，将30只C57BL/6小鼠分为5组：对照组与热打击后复温0、3、6、9 h组(n=6)。热打击前30 min小鼠禁食禁水。动物专用孵箱温度设置为39 ℃，相对湿度为60%，将热打击组小鼠放入达到热打击条件的孵箱内，每隔15 min测量小鼠核心体温，达42.5 ℃时即认为发生重症中暑[17]。将小鼠移出孵箱，复温3、6、9 h组小鼠置于原正常饲养环境，自由进食进水。
1.2.3 器官组织切片HE染色
热打击造模完成后采血，颈椎脱臼法处死小鼠，立即解剖留取肝、肾及肺组织，使用4%多聚甲醛固定，脱水、包埋制成石蜡块后切片，并进行HE染色，正置光学显微镜下观察各组织病理学改变并拍照。
1.2.4 RT-qPCR检测器官TF mRNA相对表达水平
取肝脏、肺脏及肾脏组织(10~25 mg)置于洁净EP管，彻底剪碎组织，加入1 ml Trizol吹打混匀，直至无肉眼可见大颗粒组织块，静置5~10 min，Trizol法提取组织总RNA。测定RNA浓度后用RR047A试剂盒反转录为cDNA。采用AG11701试剂盒配制反应体系，在实时定量PCR仪上机完成qPCR反应。每管反应体系为20 μl，包含10 μl 2×qPCR试剂、2 μl cDNA模板、0.4 μl引物F(10 μmol/L)、0.4 μl引物R(10 μmol/L)、7.2 μl DEPC水。实时定量PCR仪反应条件：95 ℃预变性30 s；95 ℃变性5 s，60 ℃扩增延伸反应40 s，重复40次循环。以磷酸甘油醛脱氢酶(GAPDH)作为内参照。采用2-ΔΔCt法计算TF mRNA表达水平。引物序列见表1。
1.2.5 ELISA法检测小鼠血浆TF水平
腹腔注射1%戊巴比妥钠(50 mg/kg)麻醉小鼠，摘除眼球取血法采集小鼠血液样本，置于含EDTA的EP管，在室温下以3000 r/min离心15 min，收集上层血浆，使用ELISA试剂盒检测小鼠血浆TF水平。
1.3 细胞实验
1.3.1 细胞培养及体外热打击刺激
人脐静脉内皮细胞(human umbilical vein endothelial cells，HUVECs)购自武汉普诺赛生命科技有限公司。在37 ℃、5% CO2条件下培养，使用DMEM高糖培养基(含有10%胎牛血清及1%双抗)。将细胞分为对照组与热打击复温0、3、6、9 h组，将HUVECs置于42 ℃、5% CO2培养箱中3 h进行热打击，结束后立即转移至常规培养箱(37 ℃、5% CO2)中复温。
1.3.2 细胞总蛋白及细胞培养上清蛋白提取
收集各组细胞及细胞培养上清。使用足量4 ℃预冷蛋白裂解液在冰上充分裂解细胞，10 000 r/min离心20 min后提取细胞总蛋白。将细胞培养上清以3000 r/min离心5 min，采用三氯乙酸沉淀蛋白法提取细胞培养上清蛋白[18]，用适量蛋白裂解液将蛋白沉淀重悬成蛋白溶液保存。
1.3.3 Western blotting检测HUVECs中TF的表达水平
采用BCA法检测细胞裂解蛋白溶液及细胞培养上清蛋白提取溶液中的蛋白浓度，取蛋白样品溶液，加入1/4体积的蛋白上样缓冲液(5×)混匀后，100 ℃加热5 min使蛋白变性。12.5%聚丙烯酰胺凝胶电泳分离蛋白，再进行转膜、封闭、一抗(A4395)孵育过夜、二抗(ab97080)孵育、洗膜及显影。
1.3.4 ELISA检测HUVECs培养上清中TF浓度
将细胞培养上清以1000×g离心10 min，采用ELISA法检测HUVECs培养上清中的TF浓度。
1.3.5 RT-qPCR检测HUVECs中TF mRNA的表达水平
采用Trizol法提取细胞总RNA，RT-qPCR法测定TF mRNA的相对表达水平，细胞实验所用引物序列见表2。
1.3.6 细胞免疫荧光化学法检测HUVECs细胞膜表面TF表达水平
实验前于35 mm玻底培养皿接种细胞，热打击后弃培养基，使用4 ℃预冷的PBS缓冲液洗涤细胞3次，使用4 ℃预冷的4%多聚甲醛在冰上固定细胞30 min后洗涤，依次进行抗原封闭、一抗(bs-4690R)孵育过夜、荧光二抗(ab96899)避光孵育，使用含DAPI抗荧光衰减封片剂染核，于倒置荧光显微镜下观察并拍照，使用ImageJ软件进行分析。
1.4 统计学处理
采用GraphPad Prism 8.0.2软件进行统计分析。计量资料均符合正态分布，以$\bar{x}±s$表示，多组间比较采用单因素方差分析，进一步两两比较采用Dunnett-t检验。P<0.05为差异有统计学意义。
2 结果
收起
2.1 各组小鼠不同器官TF mRNA表达水平比较
相较于对照组小鼠，热打击复温0、3 h组的肾TF mRNA (1.719±0.018、1.241±0.178 vs. 1.000±0.063)、复温3 h组的肺TF mRNA (2.444±0.511 vs. 1.000±0.106)及复温6 h组的肝TF mRNA (7.312±0.618 vs. 1.000±0.147)相对表达量均升高，差异均有统计学意义(P<0.05)。肾及肺TF mRNA表达量在热打击结束时即出现上调，肝TF mRNA表达量在复温6 h时明显上调(图1)。
2.2 各组小鼠血浆TF水平比较
ELISA检测结果显示，热打击小鼠复温0、3、6、9 h组血浆TF水平均较对照组小鼠升高[(132.426±17.920) pg/ml、(119.400±10.267) pg/ml、(107.374±13.495)pg/ml、(163.767±22.810) pg/ml vs. (75.479±13.831) pg/ml，P<0.01]，尽管复温3、6 h小鼠血浆TF水平有所下降，但热打击组小鼠整体血浆TF水平仍高于对照组(图2)。
2.3 各组小鼠器官组织病理学观察
对照组小鼠各组织未见明显病理损伤。热打击组小鼠肺、肾、肝均出现组织结构破坏、出血及炎症表现。重症中暑(即复温0 h组)小鼠肾脏皮质、髓质部均存在出血现象，肾间质小血管及毛细血管明显扩张，有充血出血、血栓形成，肾间质红细胞浸润，肾小球结构有破坏(图3A)。重症中暑小鼠肺泡结构紊乱，细支气管及终末细支气管结构破坏、出血性渗出增多，上皮组织坏死脱落，血管周围、肺间质炎症细胞及红细胞浸润增多，复温6、9 h组小鼠肺泡隔明显变细(图3B)。重症中暑小鼠肝小叶结构破坏明显，有淤血征象，光镜下可见肝小叶中央静脉及肝窦扩张充血，有红细胞充斥其间，肝细胞受压萎缩，肝索变细(图3C)。
2.4 体外热打击HUVECs持续表达分泌TF
Western blotting结果显示，与对照组比较，热打击HUVECs不同复温时长组细胞培养上清能稳定持续检测出TF，对照组细胞培养上清中未检测出TF，提示热打击诱导血管内皮细胞TF胞外分泌可能(图4A)。ELISA检测结果显示，热打击HUVECs复温0、3、6、9 h组细胞培养上清中TF含量均明显高于对照组[(36.309±4.101) pg/ml、(38.425±5.484) pg/ml、(41.655±4.380) pg/ml、(43.586±4.718) pg/ml vs. (14.996±0.254) pg/ml，P<0.01，图4B]。
细胞免疫荧光化学法检测结果显示，对照组HUVECs细胞膜表面TF荧光强度较弱，符合TF在正常生理情况下的表达水平(图5A)。热打击HUVECs复温0、3、6、9 h组细胞膜表面TF相对荧光强度明显高于对照组(3.733±0.419、2.210±0.235、2.618±0.081、4.636±0.373 vs. 1.000±0.214，P<0.01)，即热打击使HUVECs细胞膜表面结构性表达TF增强(图5B)。
2.5 各组HUVECs的TF mRNA表达水平比较
提取热打击HUVECs的mRNA进行RT-qPCR分析结果显示，热打击复温6、9 h组的TF mRNA相对表达量均明显高于对照组(1.905±0.354、2.564±0.297 vs. 1.000±0.097，P<0.01，图6)。
3 讨论
收起
重症中暑具有高发病率、高死亡率和高致残率的“三高”特征，被认为是最危险的热相关疾病，DIC作为该疾病的常见伴随高危事件，其病理机制的研究是攻克重症中暑治疗难题的关键环节之一。研究显示重症中暑DIC的发生发展与TF的表达分泌存在关联，探索TF的来源及作用是阐明重症中暑DIC发病机制的研究基础。
本研究中，重症中暑小鼠多组织出现炎症反应及凝血障碍；重症中暑早期小鼠多组织TF mRNA表达增加，且其循环内TF水平明显升高；热打击血管内皮细胞膜内TF表达分泌增加，提示血管内皮细胞可能是重症中暑TF分泌的潜在来源之一。
本研究发现，重症中暑小鼠组织发生出血性及炎性损伤，呈现炎性细胞浸润、出血及血栓形成，一定程度上符合既往研究中重症中暑导致多器官广泛出血及血栓形成的DIC病理特征[19]。有研究发现，重症中暑组织炎症反应及凝血障碍与TF表达分泌增加有关[19-20]。Huisse等[20]在2003年巴黎热浪期重症中暑临床病例中发现，患者体内循环TF水平升高。Roberts等[19]在重症中暑狒狒的血液及组织中均检测出TF含量明显上升，并与细胞凋亡相关，提示TF是重症中暑凝血反应启动的潜在重要因素。本研究采用重症中暑小鼠模型进一步证实了多组织TF mRNA表达增加，血浆分泌TF增加。此外，Bouchama等[4]在重症中暑狒狒模型中发现，重组线虫抗凝蛋白c2(组织因子/凝血因子Ⅶ抑制剂)明显改善了重症中暑动物凝血紊乱状态，进一步提示TF在重症中暑凝血紊乱中发挥作用。
血液循环中TF的潜在来源广泛，不同疾病患者体内TF的来源各不相同[21]。Franco等[22]发现，内毒素血症患者血细胞(主要为单核细胞)TF mRNA及蛋白诱导表达与高水平凝血酶-抗凝血酶复合物(凝血激活标志物)有关。脓毒症狒狒的脾脏及主动脉血管内皮细胞、脓毒症小鼠的肾小球内皮细胞均表达TF，血管内皮细胞来源TF可能也在脓毒症凝血中发挥了作用[23]。脂多糖体内外刺激单核细胞及HUVECs均可诱导TF表达，单核细胞及血管内皮细胞被看作是内毒素血症TF的两个最有可能的来源[11]。目前重症中暑表达分泌TF的细胞来源尚不十分清楚。体外热打击细胞模型显示，热打击后不同复温时间HUVECs的TF mRNA表达均有不同程度上调；细胞免疫荧光化学检测表明，HUVECs细胞膜表面TF的荧光强度持续升高。本研究还发现热打击HUVECs后，细胞培养上清分泌TF明显增加，提示热打击可能诱导细胞中TF向分泌形式转化，并可分泌至细胞外，由此推测血管内皮细胞可能是重症中暑TF表达分泌的重要来源。
热打击基于何种机制诱导血管内皮细胞分泌型TF的表达转化是下一步研究的关键。Wu等[24]提出蛋白激酶C(protein kinase C，PKC)及p38在人肺动脉内皮细胞TF的诱导表达分泌中发挥关键作用。血管内皮生长因子(vascular endothelial growth factor，VEGF)基于两种途径诱导HUVECs中TF的表达[7]，剪切力激活HUVECs中早期生长反应因子-1(early growth response protein 1，Egr-1)诱导TF表达上调[25]。多项研究表明，不同刺激诱导HUVECs表达TF均基于核因子-κB(nuclear factor kappa-B，NF-κB)[26-28]。然而热打击诱导血管内皮细胞TF表达分泌的分子机制尚未见报道，也是本文的局限性所在。
综上所述，本研究中重症中暑小鼠多组织存在炎症及凝血特征性病理损伤，验证了组织及循环TF可能参与该病理损伤过程。体外热打击可能诱导血管内皮细胞TF向分泌型转变，TF分泌增加，提示血管内皮细胞可能是重症中暑TF的来源之一。探讨热打击血管内皮细胞表达分泌TF的分子机制及有关调控措施将是未来研究的重点。
基金
收起
  • 广东省自然科学基金(2022A1515010353)
  • 军事医学创新工程专项项目(18CXZ032)
文献
收起
参考文献 引证文献
排序方式：
[1]
Edward Tom Ashworth, James David Cotter, Andrew Edward Kilding. Methods for improving thermal tolerance in military personnel prior to deployment[J]. Mil. Med. Res., 2021, 8(3): 405-423.
[2]
Yu BJ, Wang L, Peng N, et al. Study on the correlation of HMGB1 and coagulation disorder in severe heatstroke rats[J]. Med J Chin PLA, 2020, 45(3): 292-297.
余保军, 王黎, 彭娜, 等. HMGB1与重症中暑大鼠凝血紊乱的相关性分析[J]. 解放军医学杂志, 2020, 45(3): 292-297.
[3]
Jilma B, Derhaschnig U. Disseminated intravascular coagulation in heat stroke: a hot topic[J]. Crit Care Med, 2012, 40(4): 1370-1372.
[4]
Bouchama A, Al-Mohanna F, Assad L, et al. Tissue factor/factor Ⅶa pathway mediates coagulation activation in induced-heat stroke in the baboon[J]. Crit Care Med, 2012, 40(4): 1229-1236.
[5]
Mustafa KY, Omer O, Khogali M, et al. Blood coagulation and fibrinolysis in heat stroke[J]. Br J Haematol, 1985, 61(3): 517-523.
[6]
Iba T, Connors JM, Levi M, et al. Heatstroke-induced coagulopathy: Biomarkers, mechanistic insights, and patient management[J]. EClinicalMedicine, 2022, 44: 101276.
[7]
Bode M, Mackman N. Regulation of tissue factor gene expression in monocytes and endothelial cells: Thromboxane A2 as a new player[J]. Vascul Pharmacol, 2014, 62(2): 57-62.
[8]
Eisenreich A, Rauch U. Regulation and differential role of the tissue factor isoforms in cardiovascular biology[J]. Trends Cardiovasc Med, 2010, 20(6): 199-203.
[9]
Carmeliet P, Mackman N, Moons L, et al. Role of tissue factor in embryonic blood vessel development[J]. Nature, 1996, 383(6595): 73-75.
[10]
Witkowski M, Landmesser U, Rauch U. Tissue factor as a link between inflammation and coagulation[J]. Trends Cardiovasc Med, 2016, 26(4): 297-303.
[11]
Antoniak S, Mackman N. Editorial Commentary: tissue factor expression by the endothelium: coagulation or inflammation?[J]. Trends Cardiovasc Med, 2016, 26(4): 304-305.
[12]
Levi M, van der Poll T, ten Cate H. Tissue factor in infection and severe inflammation[J]. Semin Thromb Hemost, 2006, 32(1): 33-39.
[13]
Zelaya H, Rothmeier AS, Ruf W. Tissue factor at the crossroad of coagulation and cell signaling[J]. J Thromb Haemost JTH, 2018, 16(10): 1941-1952.
[14]
Bouchama A, Hammami MM, Haq A, et al. Evidence for endothelial cell activation/injury in heatstroke[J]. Crit Care Med, 1996, 24(7): 1173-1178.
[15]
Chen HS, Zhang M, Liang YX, et al. Features and function of exosomes secreted by heat-stroke vascular endothelial cells[J]. Med J Chin PLA, 2020, 45(3): 270-276.
陈怀生, 张明, 梁泳欣, 等. 热打击诱导血管内皮细胞释放的外泌体特征及其功能分析[J]. 解放军医学杂志, 2020, 45(3): 270-276.
[16]
Levi M. Burning issues surrounding inflammation and coagulation in heatstroke[J]. Crit Care Med, 2008, 36(8): 2455-2456.
[17]
Li Y, Li H, Tong HS, et al. Outer membrane vesicles derived from heatstroke-associated intestinal microbiota promote multiple organ injury in mice[J]. Microb Pathog, 2022, 170: 105653.
[18]
Link AJ, LaBaer J. Trichloroacetic acid (TCA) precipitation of proteins[J]. Cold Spring Harb Protoc, 2011, 2011(8): pdb.prot5651.
[19]
Roberts GT, Ghebeh H, Chishti MA, et al. Microvascular injury, thrombosis, inflammation, and apoptosis in the pathogenesis of heatstroke: a study in baboon model[J]. Arterioscler Thromb Vasc Biol, 2008, 28(6): 1130-1136.
[20]
Huisse MG, Pease S, Hurtado-Nedelec M, et al. Leukocyte activation: the link between inflammation and coagulation during heatstroke. A study of patients during the 2003 heat wave in Paris[J]. Crit Care Med, 2008, 36(8): 2288-2295.
[21]
Rauch U, Nemerson Y. Circulating tissue factor and thrombosis[J]. Curr Opin Hematol, 2000, 7(5): 273-277.
[22]
Franco RF, de Jonge E, Dekkers PE, et al. The in vivo kinetics of tissue factor messenger RNA expression during human endotoxemia: relationship with activation of coagulation[J]. Blood, 2000, 96(2): 554-559.
[23]
Grover SP, Mackman N. Tissue factor[J]. Arterioscler Thromb Vasc Biol, 2018, 38(4): 709-725.
[24]
Wu SQ, Aird WC. Thrombin, TNF-α, and LPS exert overlapping but nonidentical effects on gene expression in endothelial cells and vascular smooth muscle cells[J]. Am J Physiol Heart Circ Physiol, 2005, 289(2): H873-H885.
[25]
Houston P, Dickson MC, Ludbrook V, et al. Fluid shear stress induction of the tissue factor promoter in vitro and in vivo is mediated by Egr-1[J]. Arterioscler Thromb Vasc Biol, 1999, 19(2): 281-289.
[26]
Parry GCN, Mackman N. Transcriptional regulation of tissue factor expression in human endothelial cells[J]. Arterioscler Thromb Vasc Biol, 1995, 15(5): 612-621.
[27]
Crossman DC, Carr DP, Tuddenham EG, et al. The regulation of tissue factor mRNA in human endothelial cells in response to endotoxin or phorbol ester[J]. J Biol Chem, 1990, 265(17): 9782-9787.
[28]
Bevilacqua MP, Pober JS, Majeau GR, et al. Interleukin 1 (IL-1) induces biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells[J]. J Exp Med, 1984, 160(2): 618-623.
2023年第48卷第12期
PDF下载
188
75
引用本文
BibTeX
文章信息
doi: 10.11855/j.issn.0577-7402.0408.2023.0807
  • 接收时间：2023-03-21
  • 首发时间：2025-11-24
  • 出版时间：2023-12-28
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期：2023-03-21
  • 录用日期：2023-07-04
基金
Natural Science Foundation of Guangdong Province(2022A1515010353)
广东省自然科学基金(2022A1515010353)
Special Items of Military Medical Innovation Project(18CXZ032)
军事医学创新工程专项项目(18CXZ032)
作者信息
    1广州中医药大学研究生院，广东广州 510000
    2南部战区总医院重症医学科，广东广州 510000
    3南方医科大学第一临床医学院，广东广州 510000
    4广州中医药大学深圳临床医学院，广东深圳 518000
    5南部战区总医院急诊医学科，广东广州 510000

通讯作者:

周伟梁，E-mail：
童华生，E-mail：
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/jfjyxzz/CN/10.11855/j.issn.0577-7402.0408.2023.0807
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62199257
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持