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Emiliania huxleyi, the numerically dominant coccolithophore in the modern oceans and its specific lytic virus EhV exert a critical impact upon the oceanic carbon, sulfur cycle and global climate, thus serving as a key host-pathogen model system. Despite their impact on biogeochemical cycling, the transcriptional dynamics of these important oceanic events is still poorly understood. To understand the host-virus interaction in E. huxleyi-EhV system, the transcriptome of E. huxleyi BOF92 involved in virus infection was investigated by using Illumina HiSeq 2 000 high-throughput sequencing technology. Two cDNA libraries, generated 6 h and 45 h after viral infection (Exp) were compared with two libraries from the corresponding times uninfected cultures (Con). A total of 32 909 unigenes with an average length of 1 153 bp were generated. Totally 2 617 and 5 229 differentially expressed genes (DEGs) associated with viral infection were identified in 6 hpi and 45 hpi, respectively, among which 465 genes were the common DEGs in the two time points. Ten DEGs were random selected for quantitative RT-PCR (qRT-PCR) analysis, and the results confirmed that the transcriptome analysis was reliable. Furthermore, the DEGs were subject to GO and KEGG enrichment analysis. The results showed that most of the DEGs were involved in oxidation-reduction reactions, glutathione metabolism, lipid metabolism, carbohydrate metabolism and signal transduction. Some of the reactive oxygen species (ROS) scavenging genes were screened out, in which 9 genes were up-regulated and 11 genes were down-regulated. These results suggested that ROS signaling molecules might play a central role during host-virus interaction.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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