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In order to explore the effects of low temperature stress on the expression of genes related to lipid synthesis and catabolism in cobia (Rachycentron canadum), the experiment set up a normal temperature group (30.5±1.0)°C and a low temperature group (20.0±0.5)°C, and used real-time fluorescent quantitative PCR (qRT-PCR) to analyze the expression levels of 5 target genes in liver, muscle and intraperitoneal fat (IPF). The results showed that at 1 d, the expression of carnitine palmitoyl transferase-1 and hormone-sensitive lipase genes of liver, carnitine palmitoyl transferase-1, hormone-sensitive lipase and monoacylglycerol lipase genes of muscle were up-regulated (p<0.05), acetyl-CoA carboxylase and fatty acid synthase genes of liver, muscle and 5 lipid metabolism related genes of IPF were significantly down-regulated (p<0.05); at 4 d, the expression of carnitine palmitoyl transferase-1, hormone-sensitive lipase and monoacylglycerol lipase genes of liver, and hormone-sensitive lipase, monoacylglycerol lipase, acetyl-CoA carboxylase, fatty acid synthase genes of muscle and carnitine palmitoyl transferase-1, hormone-sensitive lipase, monoacylglycerol lipase, acetyl-CoA carboxylase genes of IPF were up-regulated (p<0.05), acetyl-CoA carboxylase and fatty acid synthase gene of liver were down-regulated (p<0.05); at 7 d, the expressions of carnitine palmitoyl transferase-1, hormone-sensitive lipase, monoacylglycerol lipase, acetyl-CoA carboxylase genes of liver and IPF, and hormone-sensitive lipase, monoacylglycerol lipase, acetyl-CoA carboxylase genes of muscle were up-regulated (p<0.05), carnitine palmitoyl transferase-1 gene of muscle and fatty acid synthase genes of liver were down-regulated (p<0.05). The results showed that cobia responded to low temperature stress by inhibiting lipid synthesis and metabolism, promoting lipid hydrolysis in the liver and muscle, and inhibiting the lipid hydrolysis of IPF in the early stage of low temperature stress; in the late period of low temperature stress, cobia lipid synthesis and catabolism were significantly increased, and the main tissue that used fatty acids to provide energy was transformed from the liver and muscle to liver and IPF.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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