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Isolation, identification and polyethylene-degrading characteristics of Bacillus LC-2
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Tingting Jiang1, 2, Huiping Ding1, 2, Lijuan Feng1, 2, Dahai Zhang1, 2, Ling Li1, 2, Yandong Liu1, 2, Xianguo Li1, 2, *
Haiyang Xuebao | 2021, 43(2) : 9 - 15
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Haiyang Xuebao | 2021, 43(2): 9-15
Article
Isolation, identification and polyethylene-degrading characteristics of Bacillus LC-2
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Tingting Jiang1, 2, Huiping Ding1, 2, Lijuan Feng1, 2, Dahai Zhang1, 2, Ling Li1, 2, Yandong Liu1, 2, Xianguo Li1, 2, *
Affiliations
  • 1College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China
  • 2Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Qingdao 266100, China
Published: 2021-02-25 doi: 10.12284/hyxb2021036
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Plastics accumulate in the environment and gradually break into microplastics (MPs, size below 5 mm). It poses a serious threat to the environment and human health. In this study, a strain LC-2 capable of degrading polyethylene (PE) was isolated from plastic films collected from the Licun Estuary of Qingdao. It was identified as Bacillus aquimaris by using a combination of molecular biological technique with morphological, physiological and biochemical characteristics. After the strain was isolated, it was cultured in a liquid medium for 28 days with PE as the sole carbon source. The polyethylene was subsequently separated and examined with a scanning electron microscopy, a contact angle test, a thermogravimetric analysis and a Fourier transform infrared spectrum analysis. The results showed that the weight loss of PE was about 9% after the degradation, with apparent morphologic changes on the surface of PE. It was also showed that the hydrophobicity of PE became smaller, with surface oxidation to produce −C=O functional groups. These evidences are sufficient to prove that the strain LC-2 can degrade PE.

polyethylene  /  plastic  /  degradation  /  Bacillus aquimaris
Tingting Jiang, Huiping Ding, Lijuan Feng, Dahai Zhang, Ling Li, Yandong Liu, Xianguo Li. Isolation, identification and polyethylene-degrading characteristics of Bacillus LC-2[J]. Haiyang Xuebao, 2021 , 43 (2) : 9 -15 . DOI: 10.12284/hyxb2021036
Year 2021 volume 43 Issue 2
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doi: 10.12284/hyxb2021036
  • Receive Date:2020-08-30
  • Online Date:2026-02-26
  • Published:2021-02-25
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  • Received:2020-08-30
  • Revised:2020-10-26
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    1College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China
    2Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Qingdao 266100, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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