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Coelomocytes in Apostichopus japonicus, present in coelomic fluid and water-vascular system, are considered to participate in a variety of biological functions including nutrition transport, metabolism and immunity. In the process of evisceration, coelomocytes in coelom are nearly exhausted and then recovered in a short period. The polian vesicle, as the only remained internal organ after evisceration, shows positive response that coelomocytes within it increased rapidly. Coelomocytes in coelom are nearly exhausted after evisceration, and then recovered quickly. To further investigate the function and significance of the rapid increase of coelomocytes in the early stage of regeneration after evisceration, transcriptome sequencing was performed for coelomocytes in polian vesicle and coelom of A. japonicus at 6 h after evisceration and pre-evisceration, respectively. The gene expression differences of coelomocytes in polian vesicle and coelom after evisceration were analyzed compared to those of healthy A. japonicus. These results showed that 267 genes were differentially expressed in coelomocytes of polian vesicle at 6 h after evisceration, and most of these genes were enriched into the enzyme catalytic activity subclasses according to GO functional annotation and glycine, serine and threonine metabolism pathway according to KEGG pathways annotation, respectively. Additionally, 922 differential genes were significantly expressed in coelomocytes of coelom at 6 h after evisceration, and these genes were enriched into cell adhesion and biological adhesion subclasses according to GO functional annotation and ECM-receptor interaction, TGF-β signaling pathway, FoxO signaling pathway according to KEGG pathways annotation, respectively. The results provide an important basis for further functional research and the regeneration mechanism of coelomocytes after evisceration in A. japonicus.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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