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Insulin-like growth factors (IGFs) are key regulators downstream of the growth axis and play an important role in promoting cell differentiation and growth. In order to further study the expression mechanism and possible roles of IGF-1 and IGF-2 genes in the embryonic development of Acanthopagrus latus. Our research cloned and identified the cDNA sequence of IGF-2 gene from A. latus and proceeded a biological information analysis. At the same time, based on the foundation of continual observation of the embryonic development of A. latus. RT-qPCR was carried out to explore the expression levels of IGF-1 and IGF-2 genes. The results suggested that the full length and open reading frame of IGF-2 gene are 1 736 bp and 648 bp respectively, encoding a predicted peptide of 215 amino acids. Multiple alignment showed that A. latus IGF-2 was 99.95% similar to Sparus aurata and 98.14% similarity with Pagrus auriga, as well as relatively high homology with other teleosts, indicating that conservation of IGF-2 in teleost evolutionary relationships. Through continuous observation of the embryonic development of A. latus, it was found that under the conditions of water temperature of (26.5±0.5)℃, pH of 8.0 and salinity of 28, the time from fertilization of egg to hatching of membrane of A. latus was 25.5 h. Expression analysis showed that IGF-1 and IGF-2 genes constitutively expresses in various tested stages of embryo of A. latus. The expression of IGF-1 gene showed a trend of first increasing and then decreasing, reaching a relatively high expression in the muscle effect period, and the expression of IGF-2 gene showed a trend of first increasing and then decreasing and then increasing, IGF-2 gene was expressed in the gastrula stage, the muscle effect stage and 3 days after membrane emergence. Summarizing, all the data of our study above showed that IGF-1 and IGF-2 genes may play a crucial role in embryonic development in A. latus.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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