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Knockout of Pseudoalteromonas marina pilZ gene inhibited the settlement and metamorphosis of Mytilus coruscus
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Chi Zhang1, 2, Jinsong Wang1, 2, Jinlong Yang1, 2, 3, Junbo Zhang4, 5, Rong Wan4, 5, 6, Xiao Liang1, 2, *
Haiyang Xuebao | 2022, 44(4) : 95 - 103
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Haiyang Xuebao | 2022, 44(4): 95-103
• Article •
Knockout of Pseudoalteromonas marina pilZ gene inhibited the settlement and metamorphosis of Mytilus coruscus
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Chi Zhang1, 2, Jinsong Wang1, 2, Jinlong Yang1, 2, 3, Junbo Zhang4, 5, Rong Wan4, 5, 6, Xiao Liang1, 2, *
Affiliations
  • 1. International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • 2. Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals, Shanghai 201306, China
  • 3. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China
  • 4. College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China
  • 5. National Engineering Research Center for Oceanic Fisheries, Shanghai 201306, China
  • 6. Zhoushan Branch of National Engineering Research Center for Oceanic Fisheries, Zhoushan 316014, China
Published: 2022-04-15 doi: 10.12284/hyxb2022090
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To investigate the effect of Pseudoalteromonas marina pilZ gene knockout on the biofilm formation and its influence on the settlement and metamorphosis of Mytilus coruscus larvae, ΔpilZ was constructed by homologous recombination, and the changes in bacterial density, biofilm thickness, c-di-GMP level and extracellular polymeric substances (EPS) of ΔpilZ bacteria biofilm were analyzed, as well as the regulation of the settlement and metamorphosis of M. coruscus larvae. The results showed that compared with the wild-type strain, the biofilm formed by ΔpilZ strain significantly increased the biofilm thickness, the number of bacteria, while the contents of β-polysaccharides and proteins in EPS were decreased and the settlement and metamorphosis of M. coruscus larvae were inhibited (p<0.05). There was no significant difference in c-di-GMP level, α-polysaccharide and lipid contents (p>0.05). In conclusion, P. marina pilZ gene knockout can regulate the bacterial biofilm and the content of EPS including β-polysaccharides and proteins contents, then inhibit the settlement and metamorphosis of M. coruscus larvae.

pilZ  /  biofilm  /  Mytilus coruscus  /  settlement and metamorphosis  /  extracellular polymeric substances
Chi Zhang, Jinsong Wang, Jinlong Yang, Junbo Zhang, Rong Wan, Xiao Liang. Knockout of Pseudoalteromonas marina pilZ gene inhibited the settlement and metamorphosis of Mytilus coruscus[J]. Haiyang Xuebao, 2022 , 44 (4) : 95 -103 . DOI: 10.12284/hyxb2022090
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Article Info
doi: 10.12284/hyxb2022090
  • Receive Date：2021-11-10
  • Online Date：2026-02-01
  • Published：2022-04-15
Article Data
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History
  • Received：2021-11-10
  • Revised：2021-12-27
Funding
Affiliations
    1. International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China
    2. Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals, Shanghai 201306, China
    3. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China
    4. College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China
    5. National Engineering Research Center for Oceanic Fisheries, Shanghai 201306, China
    6. Zhoushan Branch of National Engineering Research Center for Oceanic Fisheries, Zhoushan 316014, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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