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Identification of genes related to blind side hypermelanosis of Cynoglossus semilaevis based on skin transcriptome sequencing
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Na Zhao1, 3, Xiaoxu He2, 4, Lei Jia2, Chunhua Zhu1, *, Bo Zhang1, 2, *
Haiyang Xuebao | 2022, 44(2) : 57 - 66
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Haiyang Xuebao | 2022, 44(2): 57-66
• Article •
Identification of genes related to blind side hypermelanosis of Cynoglossus semilaevis based on skin transcriptome sequencing
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Na Zhao1, 3, Xiaoxu He2, 4, Lei Jia2, Chunhua Zhu1, *, Bo Zhang1, 2, *
Affiliations
  • 1. College of Fisheries, Guangdong Ocean University, Zhanjiang 524025, China
  • 2. Tianjin Fisheries Research Institute, Tianjin 300457, China
  • 3. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
  • 4. Tianjin Agricultural University, Tianjin 300384, China
Published: 2022-02-28 doi: 10.12284/hyxb2022044
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Cynoglossus semilaevis as a representative species of Pleuronectiformes, is an important marine economic fish in Chinese coastal areas. The abnormal body color of C. semilaevis has been perplexing the practitioners for a long time. There are many reasons leading to abnormal body color, among which the genetic factors are thought to be the most fundamental reasons. The present researches on hypermelanosis of the blind side of C. semilaevis mainly focus on environment, nutrition, physiology, cloning of known pigmental genes and so on, while the digging of new funtional genes is still lacking. In this study, skin samples with different colors of C. semilaevis are selected for transcriptome sequencing. Through GO and KEGG functional enrichment and comparative analysis of different genes, differential expressed genes are screened in six melanogenesis-related KEGG pathways and the top ten genes are verified by qPCR. In this study, we find five functional genes with significant changes in the hypermelanotic skin on the blind side of C. semilaevis, which refer to txndc, alox15b, ptgs2, ptgis, and atp1a2a (p<0.05). The expression levels of txndc, alox15b, ptgs2, and ptgis genes in the melanization group are higher than those in the control group. In terms of function, three of these five genes are related to arachidonic acid (AA) to some extent. This provides theoretical support for the hypothesis that nutritional regulation related to unsaturated fatty acids may be involved in the molecular mechanism of abnormal body color in C. semilaevis.

skin  /  Cynoglossus semilaevis  /  hypermelanosis  /  arachidonic acid  /  transcriptome
Na Zhao, Xiaoxu He, Lei Jia, Chunhua Zhu, Bo Zhang. Identification of genes related to blind side hypermelanosis of Cynoglossus semilaevis based on skin transcriptome sequencing[J]. Haiyang Xuebao, 2022 , 44 (2) : 57 -66 . DOI: 10.12284/hyxb2022044
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Year 2022 volume 44 Issue 2
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Article Info
doi: 10.12284/hyxb2022044
  • Receive Date：2021-08-19
  • Online Date：2026-02-01
  • Published：2022-02-28
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History
  • Received：2021-08-19
  • Revised：2021-11-02
Funding
Affiliations
    1. College of Fisheries, Guangdong Ocean University, Zhanjiang 524025, China
    2. Tianjin Fisheries Research Institute, Tianjin 300457, China
    3. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
    4. Tianjin Agricultural University, Tianjin 300384, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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