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Cloning of the McNF-κB gene of Mytilus coruscus and its role in development
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Hongyu Ren1, 2, Tiantian Liu1, 2, Youting Zhu1, 2, Jinlong Yang1, 2, Xiao Liang1, 2, *
Haiyang Xuebao | 2023, 45(9) : 141 - 151
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Haiyang Xuebao | 2023, 45(9): 141-151
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Cloning of the McNF-κB gene of Mytilus coruscus and its role in development
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Hongyu Ren1, 2, Tiantian Liu1, 2, Youting Zhu1, 2, Jinlong Yang1, 2, Xiao Liang1, 2, *
Affiliations
  • 1Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals, Shanghai Ocean University, Shanghai 201306, China
  • 2International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai 201306, China
Published: 2023-09-30 doi: 10.12284/hyxb2023114
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Nuclear factor κB (NF-κB) can regulate immunity, inflammation, apoptosis, cell proliferation, and organism development. At present, NF-κB has been well studied in vertebrates and fruit flies, while its role in shellfish is still elusive. In order to further explore the role of NF-κB in the immunity and development of mussel Mytilus coruscus, the full length McNF-κB cDNA sequence was cloned from M. coruscus. McNF-κB gene was 4087 bp long, and the open reading frame was 2 613 bp, encoding 871 amino acids and had a typical ankyrinrepeat (ANK) domain and DEATH domain. The results of amino acid sequence analysis showed that the gene had 72.76% homology with M. edulis and 66.58% with M. galloprovincialis, respectively, and was clustered with M. edulis and M. galloprovincialis in the phylogenetic tree. The real-time PCR (qRT-PCR) technology showed that the McNF-κB gene was expressed distributed in all tissues of M. coruscus, and the expression was the highest in the gill. McNF-κB gene was expressed in both the pediveliger larvae stage and the juvenile stage of M. coruscus, and the expression was significantly higher in the pediveliger larvae stage than that in the juvenile stage. After using RNA interference technology to silence the McNF-κB gene of pediveliger larvae, the larval metamorphosis rate decreased significantly, indicating that this gene was involved in regulating the metamorphosis process of M. coruscus. This study provides a basis for exploring how McNF-κB gene regulates development of M. coruscus.

Mytilus coruscus  /  NF-κB  /  gene cloning  /  larval metamorphosis
Hongyu Ren, Tiantian Liu, Youting Zhu, Jinlong Yang, Xiao Liang. Cloning of the McNF-κB gene of Mytilus coruscus and its role in development[J]. Haiyang Xuebao, 2023 , 45 (9) : 141 -151 . DOI: 10.12284/hyxb2023114
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Year 2023 volume 45 Issue 9
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Article Info
doi: 10.12284/hyxb2023114
  • Receive Date：2023-01-29
  • Online Date：2025-12-28
  • Published：2023-09-30
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History
  • Received：2023-01-29
  • Revised：2023-04-20
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Affiliations
    1Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals, Shanghai Ocean University, Shanghai 201306, China
    2International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai 201306, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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