Home Journals Articles Updates About
CN
image
收藏切换
Effects of sulfide stress on blood ${{\bf {SO}} _4^{2-}} $ concentration and SULT1B1-12 gene expression in Sinonovacula constricta
收藏切换
PDF
Huimiao Sun1, 2, Weiliang Shen3, Caifang Chen1, *, Zhihua Lin1, 4, Qingxi Han2
Haiyang Xuebao | 2023, 45(1) : 62 - 70
Less
收藏切换
Haiyang Xuebao | 2023, 45(1): 62-70
• Article •
Effects of sulfide stress on blood ${{\bf {SO}} _4^{2-}} $ concentration and SULT1B1-12 gene expression in Sinonovacula constricta
Full
Huimiao Sun1, 2, Weiliang Shen3, Caifang Chen1, *, Zhihua Lin1, 4, Qingxi Han2
Affiliations
  • 1Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
  • 2School of Marine Sciences, Ningbo University, Ningbo 315823, China
  • 3Ningbo Academy of Oceanology and Fishery, Ningbo 315012, China
  • 4Ninghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ninghai 315604, China
Published: 2023-01-01 doi: 10.12284/hyxb2023004
Outline
收藏切换

As a typical dwelled tidal shellfish, Sinonovacula constricta is often exposed to sulfide-rich environment and shows strong sulfide tolerance. The cytosolic sulfotransferase 1B1 (SULT1B1) is located at downstream of the sulfur metabolism pathway, while it is a key enzyme catalyzing the sulfation reaction and plays an important role in the biotransformation of endogenous substances such as thyroid hormones (THs). In order to study the role of ScSULT1B1-12 in sulfur resistance, the sequence characteristics were analyzed by bioinformatics method. Combined with the changes of blood ${\rm {SO}} _4^{2-} $ concentration, the spatial expression and temporal expression profiles during 72 h sulfide stress (50 μmol/L, 150 μmol/L, 300 μmol/L) were studied. The full-length cDNA of ScSULT1B1-12 gene was 1 100 bp, containing an open reading frame of 897 bp, and encoding 298 amino acids. Sequence analysis showed that ScSULT1B1-12 contains four catalytic active sites (56K, 104N, 106H, and 134A), one PAPS binding domain (YPKSGTXW) at N terminal, and one PAPS binding and dimerization domain (RKGXXGDWKNXFTVXXE) at C terminal, indicating that it was structurally able to catalyze the sulfation reaction. Spatial expression showed that ScSULT1B1-12 was highly expressed in gills, followed by the adductor muscle and hepatopancreas. Blood ${\rm {SO}} _4^{2-} $ concentration decreased, and the expression patterns of ScSULT1B1-12 also declined with fluctuation after sulfide stress, indicating that sulfate can be further transformed to sulfated donors, and ScSULT1B1-12-mediated sulfation may be inhibited to keep THs at a certain level in S. constricta, in order to strengthen the metabolic and immune functions, and make the organism adapt the adverse environment of high sulfide.

Sinonovacula constricta  /  ScSULT1B1-12  /  sulfide stress  /  temporal expression  /  sulfate ion
Huimiao Sun, Weiliang Shen, Caifang Chen, Zhihua Lin, Qingxi Han. Effects of sulfide stress on blood ${{\bf {SO}} _4^{2-}} $ concentration and SULT1B1-12 gene expression in Sinonovacula constricta[J]. Haiyang Xuebao, 2023 , 45 (1) : 62 -70 . DOI: 10.12284/hyxb2023004
Appendix
Less
Year 2023 volume 45 Issue 1
PDF
143
65
Cite this Article
BibTeX
Article Info
doi: 10.12284/hyxb2023004
  • Receive Date：2022-03-25
  • Online Date：2025-12-26
  • Published：2023-01-01
Article Data
Affiliations
History
  • Received：2022-03-25
  • Revised：2022-06-24
Funding
Affiliations
    1Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
    2School of Marine Sciences, Ningbo University, Ningbo 315823, China
    3Ningbo Academy of Oceanology and Fishery, Ningbo 315012, China
    4Ninghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ninghai 315604, China
References
Share
https://castjournals.cast.org.cn/joweb/hyxb/EN/10.12284/hyxb2023004
Share to
${{\bf {SO}} _4^{2-}} $ concentration and SULT1B1-12 gene expression in Sinonovacula constricta"> ${{\bf {SO}} _4^{2-}} $ concentration and SULT1B1-12 gene expression in Sinonovacula constricta&url=https://castjournals.cast.org.cn/joweb/hyxb/EN/10.12284/hyxb2023004">
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House