Oil and plastic pollutants are a serious threat to marine ecosystems and have even been found in the Ross Sea of the Southern Ocean. In order to obtain low-temperature alkane degrading bacteria and plastic-degrading bacteria in the region, a total of twelve sediment samples were collected in the Ross Sea area for enrichment and isolation of alkane-degrading bacteria at low-temperature, and the diversity analyses of the tetradecane-enriched communities showed that the most dominant genera were Pseudomonas, Alcanivorax, Marinomonas, Pseudoalteromonas. The polyethylene terephthalate(PET)and polyethylene(PE)was further validated using the dominant alkane-degrading bacteria. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (ATR-FTIR) demonstrated that the four pure cultures of Pseudomonas pelagia R1-05-CR3, Pseudomonas taeanensis A11-04-CA4, Halomonas titanicae A11-02-7C2 and Rhodococcus cerastii R1-05-7C3 could degrade PE effectively. The results of UPLC-MS and SEM confirmed the PET degradation by isolates of R. cerastii R1-05-7C3, Microbacterium maritypicum RA1-00-CA1, and H. titanicae A11-02-7C2. In conclusion, this study reports the diversity of the tetradecane-enriched consortia at low-temperature and plastic degrading bacteria in the offshore sediments of the Ross Sea in the Southern Ocean, which play a selfpurifying role in in-situ environmental contamination, and also provided strain resources for the biodegradation of hydrocarbon and plastic contaminants at low temperature.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |