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Microbial diversity of alkane- and plastic-degrading microbiome in offshore sediments of Ross Sea, Southern Ocean
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Sufang Zhao1, 2, Renju Liu1, 3, Chunming Dong1, Shiwei Lv1, 3, Benjuan Zhang1, Zongze Shao1, 2, 3, *
Haiyang Xuebao | 2024, 46(5) : 81 - 92
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Haiyang Xuebao | 2024, 46(5): 81-92
Microbial diversity of alkane- and plastic-degrading microbiome in offshore sediments of Ross Sea, Southern Ocean
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Sufang Zhao1, 2, Renju Liu1, 3, Chunming Dong1, Shiwei Lv1, 3, Benjuan Zhang1, Zongze Shao1, 2, 3, *
Affiliations
  • 1. Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of China; Xiamen 361005, China
  • 2. School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, China
  • 3. School of Environmental Science, Harbin Institute of Technology, Harbin 150090, China
Published: 2024-05-31 doi: 10.12284/hyxb2024066
Outline
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Oil and plastic pollutants are a serious threat to marine ecosystems and have even been found in the Ross Sea of the Southern Ocean. In order to obtain low-temperature alkane degrading bacteria and plastic-degrading bacteria in the region, a total of twelve sediment samples were collected in the Ross Sea area for enrichment and isolation of alkane-degrading bacteria at low-temperature, and the diversity analyses of the tetradecane-enriched communities showed that the most dominant genera were Pseudomonas, Alcanivorax, Marinomonas, Pseudoalteromonas. The polyethylene terephthalate(PET)and polyethylene(PE)was further validated using the dominant alkane-degrading bacteria. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (ATR-FTIR) demonstrated that the four pure cultures of Pseudomonas pelagia R1-05-CR3, Pseudomonas taeanensis A11-04-CA4, Halomonas titanicae A11-02-7C2 and Rhodococcus cerastii R1-05-7C3 could degrade PE effectively. The results of UPLC-MS and SEM confirmed the PET degradation by isolates of R. cerastii R1-05-7C3, Microbacterium maritypicum RA1-00-CA1, and H. titanicae A11-02-7C2. In conclusion, this study reports the diversity of the tetradecane-enriched consortia at low-temperature and plastic degrading bacteria in the offshore sediments of the Ross Sea in the Southern Ocean, which play a selfpurifying role in in-situ environmental contamination, and also provided strain resources for the biodegradation of hydrocarbon and plastic contaminants at low temperature.

Ross Sea  /  alkanes  /  polyethylene  /  polyethylene terephthalate  /  biodegradation  /  cold-adapted microorganisms
Sufang Zhao, Renju Liu, Chunming Dong, Shiwei Lv, Benjuan Zhang, Zongze Shao. Microbial diversity of alkane- and plastic-degrading microbiome in offshore sediments of Ross Sea, Southern Ocean[J]. Haiyang Xuebao, 2024 , 46 (5) : 81 -92 . DOI: 10.12284/hyxb2024066
Year 2024 volume 46 Issue 5
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Article Info
doi: 10.12284/hyxb2024066
  • Receive Date:2023-11-11
  • Online Date:2025-11-26
  • Published:2024-05-31
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  • Received:2023-11-11
  • Revised:2024-03-11
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Affiliations
    1. Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of China; Xiamen 361005, China
    2. School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, China
    3. School of Environmental Science, Harbin Institute of Technology, Harbin 150090, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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