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Research progress on interspecies electron transfer mechanisms and their regulation in denitrification systems
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Jiaxin JIANG1, Fang FANG1, Jialing ZHANG2, Jingyang LUO1, Jiashun CAO1
Environmental Engineering | 2026, 44(3) : 46 - 57
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Environmental Engineering | 2026, 44(3): 46-57
Research progress on interspecies electron transfer mechanisms and their regulation in denitrification systems
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Jiaxin JIANG1, Fang FANG1, Jialing ZHANG2, Jingyang LUO1, Jiashun CAO1
Affiliations
  • 1Key Laboratory of Shallow Lake Integrated Management and Resource Development,Ministry of Education,College of Environment,Hohai University,Nanjing 210098,China
  • 2Nanjing Research Institute of Environmental Sciences,Ministry of Ecology and Environment,Nanjing 210042,China
Published: 2026-03-22 doi: 10.13205/j.hjgc.202603004
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Denitrification is a critical process for advanced nitrogen removal in wastewater treatment, fundamentally governed by microbially driven electron transfer and electron allocation. As research has shifted from macroscopic treatment metrics toward microscopic regulation, elucidating denitrification mechanisms from an electron-flow perspective has emerged as a major research frontier. This review systematically summarized the theoretical framework of electron flow in denitrification systems, compared intracellular electron transport pathways and energy allocation characteristics between heterotrophic and autotrophic denitrifiers, and highlighted the central role of the quinone pool in electron collection and redistribution. Furthermore, from the perspective of interspecies microbial interactions, recent advances in indirect interspecies electron transfer (IIET) and direct interspecies electron transfer (DIET) were summarized, and competitive as well as cooperative interactions among microorganisms in mixed systems during electron donor and electron acceptor utilization were analyzed. Building on this framework, the impacts of carbon source characteristics, pH, oxidation-reduction potential (ORP), and coexisting contaminants on electron transport chains and electron allocation pathways were further discussed. Finally, in light of current limitations in the in situ quantification of electron fluxes, future research directions were proposed, including the development of multi-scale in situ characterization techniques, novel electron-conductive materials, and intelligent electron-flow regulation models.

denitrification  /  electron allocation  /  intracellular/extracellular electron transport chain  /  microbial competition mechanism
Jiaxin JIANG, Fang FANG, Jialing ZHANG, Jingyang LUO, Jiashun CAO. Research progress on interspecies electron transfer mechanisms and their regulation in denitrification systems[J]. Environmental Engineering, 2026 , 44 (3) : 46 -57 . DOI: 10.13205/j.hjgc.202603004
Year 2026 volume 44 Issue 3
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doi: 10.13205/j.hjgc.202603004
  • Receive Date:2026-02-06
  • Online Date:2026-06-25
  • Published:2026-03-22
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  • Received:2026-02-06
  • Revised:2026-02-27
  • Accepted:2026-03-02
Affiliations
    1Key Laboratory of Shallow Lake Integrated Management and Resource Development,Ministry of Education,College of Environment,Hohai University,Nanjing 210098,China
    2Nanjing Research Institute of Environmental Sciences,Ministry of Ecology and Environment,Nanjing 210042,China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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