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Research progress of the CRISPR-Cas system in the detecting pathogen nucleic acids
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Yao DU1, 2, Hongdan GAO1, 2, Jiakun LIU3, 4, Xiaorong LIU2, Zhihao XING2, Tao ZHANG5, Dongli MA2
Synthetic Biology Journal | 2024, 5(1) : 202 - 216
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Synthetic Biology Journal | 2024, 5(1): 202-216
• Invited Review •
Research progress of the CRISPR-Cas system in the detecting pathogen nucleic acids
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Yao DU1, 2, Hongdan GAO1, 2, Jiakun LIU3, 4, Xiaorong LIU2, Zhihao XING2, Tao ZHANG5, Dongli MA2
Affiliations
  • 1 School of Laboratory Medicine，Bengbu Medical College，Bengbu 233030，Anhui，China
  • 2 Pediatric Research Institute，Shenzhen Children’s Hospital，Shenzhen 518034，Guangdong，China
  • 3 Shenzhen Institute of Synthetic Biology，Shenzhen Institute of Advanced Technology，Chinese Academy of Sciences，Shenzhen 518055，Guangdong，China
  • 4 Shenzhen Hapmic Biotechnology Company limited，Shenzhen 518057，Guangdong，China
  • 5 Department of Pathogenic Biology，Bengbu Medical College，Bengbu 233030，Anhui，China
Published: 2024-02-29 doi: 10.12211/2096-8280.2022-068
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The CRISPR-Cas system consists of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins, which has become the focus of molecular diagnosis because it recognizes and cleaves specific DNA or RNA sequences. Using Cas proteins (Cas12, Cas13, Cas14, Cas3, etc.) combined with signal amplification and transformation techniques (fluorescence, potentiometric, colorimetric, lateral flow assay, etc.), researchers have developed many diagnostic platforms with high sensitivity, good specificity, and low cost, which provide a new tool for detecting pathogen nucleic acids. This review presents the biological mechanism and classification of the CRISPR-Cas system, and also summarizes existing technologies for detecting pathogenic nucleic acids based on the trans-cleavage activity of Cas proteins, commenting their properties, functions and application scenarios, with future applications prospected based on the functional characteristics of the CRISPR-Cas system, which is expected to become an ideal detection platform for other multiple targets.

CRISPR-Cas system  /  Cas12 and Cas13  /  pathogen  /  nucleic acid detection  /  biosensor
Yao DU, Hongdan GAO, Jiakun LIU, Xiaorong LIU, Zhihao XING, Tao ZHANG, Dongli MA. Research progress of the CRISPR-Cas system in the detecting pathogen nucleic acids[J]. Synthetic Biology Journal, 2024 , 5 (1) : 202 -216 . DOI: 10.12211/2096-8280.2022-068
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Year 2024 volume 5 Issue 1
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Article Info
doi: 10.12211/2096-8280.2022-068
  • Receive Date：2022-11-26
  • Online Date：2025-07-07
  • Published：2024-02-29
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History
  • Received：2022-11-26
  • Revised：2023-02-15
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Affiliations
    1 School of Laboratory Medicine，Bengbu Medical College，Bengbu 233030，Anhui，China
    2 Pediatric Research Institute，Shenzhen Children’s Hospital，Shenzhen 518034，Guangdong，China
    3 Shenzhen Institute of Synthetic Biology，Shenzhen Institute of Advanced Technology，Chinese Academy of Sciences，Shenzhen 518055，Guangdong，China
    4 Shenzhen Hapmic Biotechnology Company limited，Shenzhen 518057，Guangdong，China
    5 Department of Pathogenic Biology，Bengbu Medical College，Bengbu 233030，Anhui，China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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Genus		 种数
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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