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Identification and characterization of a promiscuous pentacyclic triterpenoid O-glycosyltransferase from Phytolacca americana
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Yi Zhang1, Jinxin Wang1, Yiqing Zhu1, Yunheng Shen1, Hongyuan Dong1, Haiyang Yu1, Zirui An1, Weidong Zhang1, 2, *, Yu Zhang2, *, Zhimin Hu1, *
Chinese Journal of Natural Medicines | 2026, 24(8) : 999 - 1005
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Chinese Journal of Natural Medicines | 2026, 24(8): 999-1005
• Original Article •
Identification and characterization of a promiscuous pentacyclic triterpenoid O-glycosyltransferase from Phytolacca americana
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Yi Zhang1, Jinxin Wang1, Yiqing Zhu1, Yunheng Shen1, Hongyuan Dong1, Haiyang Yu1, Zirui An1, Weidong Zhang1, 2, *, Yu Zhang2, *, Zhimin Hu1, *
Affiliations
  • 1Department of Phytochemistry, School of Pharmacy, Naval Medical University, Shanghai 200433, China
  • 2Shanghai Frontiers Science Center for Chinese Medicine Chemical Biology, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
About Author:

∆

These authors contributed equally to this work.

Published: 2026-08-20 doi: 10.1016/S1875-5364(26)61198-3
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Glycosyltransferases (GTs) are key enzymes in the glycosylation of plant secondary metabolites, primarily catalyzing the transfer of a sugar moiety from an activated donor to a specific acceptor molecule. Triterpenoid saponins, an abundant and diverse group of natural products, are composed of triterpenoid aglycones and one or more sugar chains, under the catalysis of GTs. Phytolacca Radix is a traditional Chinese medicine containing over 40 triterpenoid saponins with pharmacological values. However, the identification of glycosyltransferases related to triterpenoid saponin synthesis from Phytolacca species remains scarce. In this study, a novel glycosyltransferase, PamUGT, was identified from Phytolacca americana. PamUGT exhibits catalytic activity toward 28-/30-COOH of pentacyclic triterpenoids, and it exhibits a preference for the C-30 position when both carboxyl groups are available. To our knowledge, PamUGT is the first glycosyltransferase identified in Phytolacca species that can catalyze triterpenoids. Further analysis of substrate specificity revealed that PamUGT catalyzes the glycosylation of a wide range of compounds, including triterpenoids, flavonoids, diterpenoids, alkaloids, and phenolic acids. Our findings identify a highly promiscuous glycosyltransferase, offering a valuable enzymatic tool for modifying diverse natural products.

Phytolacca americana  /  Pentacyclic triterpenoid  /  Glycosylation  /  Glycosyltransferase
Yi Zhang, Jinxin Wang, Yiqing Zhu, Yunheng Shen, Hongyuan Dong, Haiyang Yu, Zirui An, Weidong Zhang, Yu Zhang, Zhimin Hu. Identification and characterization of a promiscuous pentacyclic triterpenoid O-glycosyltransferase from Phytolacca americana[J]. Chinese Journal of Natural Medicines, 2026 , 24 (8) : 999 -1005 . DOI: 10.1016/S1875-5364(26)61198-3
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Funding
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  • National Natural Science Foundation of China(82304667)
  • Science and Technology Commission of Shanghai Municipality(23YF1457800)
Appendix
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Year 2026 volume 24 Issue 8
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71
11
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Article Info
doi: 10.1016/S1875-5364(26)61198-3
  • Receive Date：2025-10-09
  • Online Date：2026-08-20
  • Published：2026-08-20
Article Data
Affiliations
History
  • Received：2025-10-09
  • Revised：2025-12-14
  • Accepted：2026-02-22
Funding
National Natural Science Foundation of China(82304667)
Science and Technology Commission of Shanghai Municipality(23YF1457800)
Affiliations
    1Department of Phytochemistry, School of Pharmacy, Naval Medical University, Shanghai 200433, China
    2Shanghai Frontiers Science Center for Chinese Medicine Chemical Biology, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

Corresponding:

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E-mail address: (W. Zhang)
(Y. Zhang)
(Z. Hu)
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https://castjournals.cast.org.cn/joweb/cjnm/EN/10.1016/S1875-5364(26)61198-3
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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