To screen cold-tolerant germplasm resources of Pennisetum， clarify the cold resistance differences among different germplasms， and provide a theoretical basis for cold resistance breeding， 118 Pennisetum germplasm resources were used as experimental materials in this study， and comprehensive evaluation of cold resistance was carried out via field low-temperature stress treatment combined with eight cold resistance-related indicators. The results revealed that the cold resistance-related traits of the tested germplasms possessed abundant genetic diversity， which could provide sufficient genetic basis for cold resistance breeding. Chlorophyll content was significantly correlated with regrowth seedling number and stem sugar content， serving as key factors affecting cold resistance differences among Pennisetum germplasms. Principal component analysis simplified eight indicators into four principal components with a cumulative contribution rate of 80.924%， with chlorophyll reduction value after low-temperature stress， chlorophyll content under low temperature， regrowth seedling number and stem sugar content identified as the core indicators. Based on cluster analysis， all the germplasms were classified into three groups， and a total of 30 excellent cold-resistant germplasms including Pp20230630-3， Pp20230628-5 and Pp014 were screened out using membership function method. This study provides important germplasm resources and theoretical references for cold-resistant germplasm innovation and new variety breeding of Pennisetum.

Pennisetum germplasm resources  /  Low-temperature stress  /  Cold resistance evaluation  /  Membership function