Establishing an efficient genome editing system in forage oats has long been a crucial approach for oat germplasm innovation. However， due to the hexaploid nature of forage oats， genetic transformation efficiency is relatively low， posing significant challenges in creating new germplasms using genome editing technology. Here， the genetic transformation efficiency of forage oats was significantly improved through the exogenous addition of small peptides. Simultaneously， genome editing efficiency was enhanced by employing T5E fusion proteins， leading to the development of an independent intellectual property genome editing technology system for forage oats with an editing efficiency of 20%. Additionally， a multi-genome editing system for forage oats was established with an editing efficiency of 23%， and a single base editing system for forage oats was developed with an editing efficiency of 21.4%. The establishment of highly efficient genetic transformation and genome editing systems for oats holds significant importance for gene function research and germplasm innovation in forage oats.

Genome editing  /  CRISPR/Cas9  /  Forage oat  /  Peptide