The MYB （V-myb Avian myeloblastosis viral Oncogene Homolog） gene family represents one of the most extensive groups of transcription factors in plants， playing a crucial role in various processes including plant growth， development， and stress responses. This study utilized Medicago sativa‘Pianguan’ to investigate the characteristics of the MsMYB44 gene through methodologies such as gene cloning， bioinformatics analysis， subcellular localization， and expression pattern analysis. The findings revealed that the MsMYB44 gene of Medicago sativa‘Pianguan’ comprised 966 base pairs and encoded a total of 321 amino acids. The MsMYB44 protein exhibited a high degree of homology to the MtMYB44 protein in Medicago truncatula and was localized within the nucleus. The expression of the MsMYB44 gene was observed in various tissues of Medicago sativa‘Pianguan’ across different developmental stages， indicating tissue-specific expression patterns. The MsMYB44 gene was responsive to drought， salt stress， and abscisic acid （ABA） treatment. Under drought conditions， the relative expression level of MsMYB44 initially increased， subsequently decreased， and then increased again， peaking at 12 hours. In response to salt stress， the relative expression level of MsMYB44 gradually increased after 48 hours， reaching its maximum at 72 hours. Following ABA treatment， the relative expression level of MsMYB44 peaked at 12 hours and then progressively declined. These findings would provide a theoretical framework for elucidating the role of the MsMYB44 gene of Medicago sativa in mediating the abiotic stress response.

MsMYB44  /  Medicago sativa‘Pianguan’  /  Cloning identification  /  Subcellular localization  /  Expression pattern  /  Abiotic stress