To elucidate the potential roles of the quinoa PHD-finger gene family in development and stress adaptation， this study was conducted based on the reference genome of quinoa （Chenopodium quinoa Willd.） and transcriptomic datasets from multiple sources. Genome-wide identification， chromosomal localization， phylogenetic analysis， gene structure characterization， conserved motif analysis， cis-acting element prediction， duplication event analysis， and expression profiling were performed， followed by validation using qRT-PCR. A total of 69 CqPHD-finger members were identified and found to be unevenly distributed across 18 chromosomes. The expansion of this gene family was mainly driven by segmental duplication. Phylogenetic analysis classified these members into 13 subgroups， and their promoters were enriched in cis-acting elements associated with light signaling， hormone responses， and stress responses. Members of group 9 clustered closely with proteins related to abiotic stress responses， whereas members of group 10 showed a close phylogenetic relationship with proteins involved in tapetum and pollen development. Expression analysis revealed that this gene family exhibited tissue-specific expression patterns in meristems， floral organs， and mature seeds， and showed pronounced responses to high temperature， drought， and long-day conditions. qRT-PCR analysis demonstrated that CqPHD-finger29， CqPHD-finger66， CqPHD-finger30， and CqPHD-finger23 exhibited significant photoperiod responsiveness， suggesting that they might participate in the regulation of photoperiod-dependent flowering in quinoa. This study provides a theoretical basis for further elucidating the functions of the PHD-finger gene family in quinoa reproductive development， stress responses， and photoperiodic regulation.

Quinoa  /  PHD-finger  /  Transcription factor  /  Bioinformatics  /  Gene family