Laccase （LAC）， as an important member of the ceruloplasmin protein family， plays a key role in the regulation of plant growth and development and the response to abiotic stress. In this study， the local superior germplasm of Medicago sativa ‘Pianguan’ in Shanxi Province was used as the experimental material. The MsLAC17 gene was cloned by PCR. And was analyzed by bioinformatics means and fluorescence quantitative PCR technology. The tissue specificity expression of MsLAC17 gene and its response under different abiotic stresses were systematically explored. The results showed that the length of the MsLAC17 gene is 1782 bp， encoding 593 amino acids. The relative molecular mass of this protein is 66.26 kDa， and its theoretical isoelectric point is 10.05. It is a hydrophilic protein mainly located in chloroplasts and mitochondria. The results of phylogenetic analysis indicated that the LAC17 protein of Medicago sativa ‘Pianguan’ is closely related to that of Medicago truncatula. Fluorescence quantitative PCR detection revealed that the expression level of the MsLAC17 gene was the highest in the stem， and responded to salt， drought and ABA treatment. It is speculated that this gene may play a role in regulating the response of Medicago sativa ‘Pianguan’ to abiotic stress. The results of this study provide an important basis for further clarifying the biological function of the MsLAC17 gene.

Medicago sativa ‘Pianguan’  /  Abiotic stress  /  MsLAC17  /  Expression pattern