Aiming at the problems of a single evaluation system for the regeneration ability of alfalfa （Medicago sativa L.） under high-frequency cutting and unclear mechanisms of varietal differentiation， this study took 9 alfalfa varieties as materials， determined the plant height， growth curve， biomass， photosynthetic parameters， and non-structural carbohydrate content after cutting， and conducted a comprehensive evaluation using the membership function method. The results indicated significant differences in regrowth performance among the various cultivars. Among them， ‘WL363HQ’ exhibited outstanding performance， with a mean daily plant height increase of 1.93 cm， which was significantly higher than that of the other tested cultivars. In contrast， the ‘Beiji Star’ cultivar demonstrated the weakest overall performance. Through systematic evaluation of the regrowth capacity differences among the alfalfa cultivars， this study confirmed that under high-frequency cutting conditions， ‘WL363HQ’ possessed the strongest regrowth ability among the nine tested cultivars， while ‘Beiji Star’ showed the weakest regrowth capacity. These findings provide a theoretical basis for the selection and breeding of alfalfa cultivars with enhanced tolerance to frequent cutting.

Medicago sativa L.  /  Cutting  /  Regeneration capacity  /  Photosynthetic characteristics  /  Biomass