Latest ArticlesThe core-shell structured Au@Bi2S3 nanorods have been prepared through direct in-situ growth of Bi2S3 at the surface of pre-synthesized gold nanorods. The product was characterized by X-ray diffraction, transmission electron microscopy and energy-dispersive X-ray spectroscopy. Then the obtained Au@Bi2S3 nanorods were coated onto glassy carbon electrode to act as a scaffold for fabrication of electrochemical DNA biosensor on the basis of the coordination of -NH2 modified on 5'-end of probe DNA and Au@Bi2S3. Electrochemical characterization assays demonstrate that the Au@Bi2S3 nanorods behave as an excellent electronic transport channel to promote the electron transfer kinetics and increase the effective surface area by their nanosize effect. The hybridization experiments reveal that the Au@Bi2S3 matrix-based DNA biosensor is capable of recognizing complementary DNA over a wide concentration ranging from 10 fmol/L to 1 nmol/L. The limit of detection was estimated to be 2 fmol/L (S/N=3). The biosensor also presents remarkable selectivity to distinguish fully complementary sequences from basemismatched and non-complementary ones, showing great promising in practical application.
TNFR1-associated death domain protein (TRADD) with arginine N-GlcNAcylation is a novel and structurally unique posttranslational modification (PTM) glycoprotein that blocks the formation of death-inducing signaling complex (DISC), orchestrating host nuclear factor kB (NF-kB) signaling in entero-pathogenic Escherichia coli (EPEC)-infected cells. This particular glycosylated modification plays an extremely vital role for the effective colonization and pathogenesis of pathogens in the gut. Herein we describe the total synthesis of TRADD death domain (residues 195-312) with arginine235 N-GlcNAcylation (Arg-GlcNAc TRADD (195-312)). Two longish peptidyl fragments of the wild-type primary sequence were obtained by robust, microwave-assisted, highly efficient, solid-phase peptide synthesis (SPPS), the N-GlcNAcylated sector was built by total synthesis and attached specifically to resinbound peptide with an unprotected ornithine residue via silver-promoted on-resin guanidinylation, Arg-GlcNAc TRADD (195-312) was constructed by hydrazide-based native chemical ligation (NCL). The facile synthetic strategy is expected to be generally applicable for the rapid synthesis of other proteins with Arg-GlcNAc modification and to pave the way for the related chemically biological study.
Hard carbon is regarded as promising anode materials for potassium-ion batteries (KIBs) owing to their low price and easy availability. However, the limited rate capability still needs to be improved. Herein, we demonstrate the fabrication of oxygen/sulfur co-doped hard carbon through a facile hydrolyzationsulfuration process of skimmed cotton. The simultaneous dopants significantly improve potassium ion diffusion rate. When served as the anode for KIBs, this hydrolyzed hard carbon delivered a high reversible capacity (409 mAh/g at 0.1 A/g), superior rate capability (135 mAh/g at 2 A/g) and excellent cyclability (about 120 mAh/g overt 500 cycles at 2 A/g). This work provides a facile strategy to prepare low-cost doped-hard carbon with superior potassium storage property.
Undesired adsorption of proteins brings big troubles to marine structures. The settled proteins change the physical and chemical properties of the surfaces, which allow marine fouling organisms to settle down on the structures. Therefore, to understand the adsorption mechanism of proteins is very helpful to find an environment-friendly solution against biofouling. Many approaches have been developed to study protein adsorption, but most of them are insufficient to give the chemical interaction information between proteins and surfaces. Fourier transform infrared spectroscopy with attenuated total reflection (FTIR-ATR) is an efficient, fast and non-destructive method for in situ surface measurement, which greatly minimizes the interference of water to infrared spectra, because of the very small depth of penetration of the evanescent wave. In this paper, an in situ FTIR-ATR technology was used to investigate the adsorption process of trypsin on a bare ZnSe surface and on a TiO2 coated ZnSe surface, and the effect of calcium cation strength and ultraviolet light irradiation on the secondary structure of trypsin were also evaluated. FTIR spectra of trypsin showed that Amide Ⅰ band red shift and Amide Ⅱ band blue shift in aqueous environment on both surfaces compared with the dry trypsin powder, and the addition of calcium cations further changed the Amide bands position, which indicated that the change of the secondary structure could be interfered by the environment. The hydrogen bond formation between water and trypsin, the interaction between surface and trypsin, the interaction between hydrated calcium cations and trypsin, are major factors to change the secondary structure of trypsin, and UV light irradiation also showed its influence for the secondary structure.
Cysteine (Cys) plays a pivotal role in many physiological and pathological processes, including detoxification and protein synthesis. The abnormal levels of Cys are linked to many diseases. In this study, a novel red-emitting off-on fluorescent probe Cys-TCF was masterly constructed for discriminative detection of Cys. After a series of experimental assessment, Cys-TCF displayed higher selectivity and sensitivity for Cys over other biothilols with a low detection limit (0.04 μmol/L). More notably, the probe was also successfully applied to image Cys in live cells and live zebrafishes with low cytotoxicity.
Gold nanoparticles functionalized hollow mesoporous Prussian blue nanoparticles (Au@HMPB NPs) were synthesized and its peroxidase-like activity was explored for electrochemical probe. The Au@HMPB NPs can reduce H2O2 at low detection potential of -0.1 V with high sensitivity. After physically adsorption of antibodies onto the gold nanoparticle surface, the functionalized nanoparticles were turned into immuno-probe. The soluble α-chain of interleukin-2 (IL-2) receptor (sCD25) was chosen as a model protein biomarker to test the performance of the probe. sCD25 in the samples were captured and enriched by capture anti-CD25 antibody functionalized magnetic nanospheres. Detection antibody functionalized Au@HMPB can then be linked onto the nanospheres and generate electrochemical current towards H2O2 reduction. The electrochemical responses to 1 mmol/L H2O2 was increased with the increasing concentration of CD25.
Hard carbon is promising anode for potassium-ion batteries (PIBs), however, the poor rate capability hinders its development as potential anode. To address this question, we design a sulfur-doped porous hard carbon (S-HC) for PIBs through the combination of structural design and composition adjustment. The as-designed S-HC exhibits a long cycling life with ~191 mAh/g after 300 cycles at 1 A/g, and an excellent rate capability with ~100 mAh/g at 5 A/g, which was attributed to its structural characteristics and compositions. The S-HC demonstrates to be promising anode in the future.
In clinical cancer research, it is quite promising to develop multimodal synergistic therapeutic strategies. Photodynamic and photothermal synergistic therapy is a very desirable multimodal therapy strategy. Herein, we report a facile and simple method to construct a nanotherapeutic agent for photodynamic and photothermal therapy. This nanotherapeutic agent (ZnO@Ce6-PDA) is composed of a ZnO nanoparticle core, an interlayer of photosensitizer chlorin e6 (Ce6) and an outer layer of polydopamine (PDA). Due to the existence of Ce6, the ZnO@Ce6-PDA can efficiently generate singlet oxygen (1O2) under 660 nm laser irradiation. Moreover, the ZnO@Ce6-PDA can serve as a photothermal agent, because of the excellent photothermal conversion efficiency of the PDA coating layer in the presence of 780 nm laser. Experiment results demonstrated that the designed nanotherapeutic agent had outstanding phototoxicity upon the combination of laser irradiation at 660 and 780 nm. Thus, our work proves that the ZnO@Ce6-PDA is a promising photodynamic/photothermal dual-modal nanotherapeutic agent for enhanced cancer therapy.
We describe a simple method to prepare magnetic responsive polydivinylbenzene (PDVB) nanofiber composites by precipitated cationic living polymerization in the present of oleic acid capped Fe3O4 nanoparticles (NPs). The Fe3O4 NPs are encapsulated with the PDVB forming dendrites, from which thin nanofibers are grown in the tip-growth mode. The thin nanofibers are interwoven with the thick nanofibers forming robust composite network. The composites are magnetic responsive and highly efficient to gel almost all chemicals. Separation of the gelled chemicals from water becomes easier with a magnet. The performance is promising for magnetic collection of chemical spills.
A coumarinocoumarin-based fluorescent probe, JCCA, was developed for the detection of N2H4. JCCA exhibited a fast turn-on fluorescence enhancement in response to N2H4 with good selectivity, sensitivity and a detection limit of 7.4 nmol/L. Significantly, JCCA displayed a good capability for visualizing N2H4 in living cells and zebra fish.
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