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Comprehensive profiling of CTP-binding proteins using a biotinylated CTP affinity probe
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Mengting Pan, Yunming Liu, Xiaofang Zheng, Meijuan Zhou, Changjun You*, Xiaoxia Dai*
Chinese Chemical Letters | 2021, 32(11) : 3479 - 3482
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Chinese Chemical Letters | 2021, 32(11): 3479-3482
• Communication •
Comprehensive profiling of CTP-binding proteins using a biotinylated CTP affinity probe
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Mengting Pan, Yunming Liu, Xiaofang Zheng, Meijuan Zhou, Changjun You*, Xiaoxia Dai*
Affiliations
  • State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Molecular Science and Biomedicine Laboratory, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha 410082, China
Published: 2021-11-15 doi: 10.1016/j.cclet.2021.05.057
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Recent studies have shown that CTP may act as a ligand to regulate the activity of its target proteins in many biological processes. However, proteome-wide identification of CTP-binding proteins remains challenging. Here, we employed a biotinylated CTP affinity probe coupled with stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics approach to capture, identify and quantify CTP-binding proteins in human cells. By performing two types of competitive SILAC experiments with high vs. low concentrations of CTP probe (100 vs. 10 µmol/L) or with CTP probe in the presence of free CTP, we identified 90 potential CTP-binding proteins which are involved in a variety of biological processes, including protein folding, nucleotide binding and cell-cell adhesion. Together, we developed a chemical proteomic method for uncovering the CTP-binding proteins in human cells, which could be widely applicable for profiling CTP-binding proteins in other biological samples.

CTP-binding proteins  /  CTP affinity probe  /  Mass spectrometry  /  SILAC  /  Quantitative proteomics
Mengting Pan, Yunming Liu, Xiaofang Zheng, Meijuan Zhou, Changjun You, Xiaoxia Dai. Comprehensive profiling of CTP-binding proteins using a biotinylated CTP affinity probe[J]. Chinese Chemical Letters, 2021 , 32 (11) : 3479 -3482 . DOI: 10.1016/j.cclet.2021.05.057
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CTP is an essential nucleotide that is required for the biosynthesis of nucleic acids [1] and protein glycosylation [2]. In addition, it also serves as a critical precursor during the synthesis of membrane phospholipids [3] and participates in cellular signal communication [4]. While ATP serves as the phosphate donor for most kinases, numerous studies have found CTP-dependent kinases that utilize CTP as phosphate donor, such as yeast diacylglycerol kinase [5]. Moreover, CTP synthase (CTPS1) has emerged as a potential therapeutic target for many diseases such as cancer [6]. CTP can bind and inhibit CTPS1 through competition with UTP for a shared 5′-triphosphate-binding pocket [7]. Furthermore, ParB-like proteins in bacteria are another important class of CTP-dependent molecular switches which are involved in DNA segregation during cell division [8]. Despite the importance of CTP-binding in critical metabolic processes, the proteome-wide study of CTP-binding proteins remains under investigation.
Recent advances in mass spectrometry (MS) instruments and analytical methods render MS-based proteomics a powerful tool for rapidly profiling the whole proteome from diverse biological samples [9]. Coupled with stable isotope labeling with amino acids in cell culture (SILAC) technique, MS can be used to identify and accurately quantify thousands of proteins from complex biological samples. So far, various affinity enrichment methods, such as biotin-based pulldown assay and activity-based protein profiling (ABPP), have been developed to capture specific group of proteins which have similar structure and function from complex biological system [10]. For instance, acyl phosphate-containing ABPP probes have been widely used for characterizing ATP- and GTP-binding proteins [11, 12]. Additionally, competition assays which use free ATP or different concentrations of ATP probes have also been employed to effectively eliminate the non-specific ATP-binding proteins from whole cell lysates [11, 13]. In this study, we report a proteome-wide analysis of CTP-binding proteins in human cells using a specific biotin-CTP probe coupled with SILAC-based mass spectrometry strategy, which provides new ideas about the biological roles of CTP.
Many ATP- and GTP-binding proteins have a phosphate-binding loop (P-loop) which contains a glycine-rich sequence and a conserved lysine [14]. Acyl phosphate-containing ATP and GTP probes have been widely used for targeting the lysine residues in P-loop of target proteins [15, 16]. In this study, we designed a biotinylated CTP affinity probe which harbors three components: CTP, a binding moiety, which specifically recognizes the active sites of CTP-binding proteins; biotin, an enrichment moiety, which facilitates the enrichment of target proteins; γ-aminobutyric acid, a linker, which connects the enrichment moiety to CTP and acts as a spacer to minimize steric hindrance (Fig. 1). The biotinylated CTP affinity probe was synthesized and purified for the enrichment of CTP-binding proteins in human cells (Figs. S1 and S2 in Supporting information). We speculate that the CTP probe could play a role like ATP and GTP acyl-phosphate probe [17]: upon interacting with CTP-binding proteins, CTP moiety binds to the active sites and then the acyl-phosphate component of probe reacts with the conserved ε-amino group of lysine residue located at or near the binding pocket to form a stable amide bond (Fig. S3 in Supporting information).
To verify this hypothesis, we incubated the CTP affinity probe with different concentrations of lysine, ranging from 100 µmol/L to 20 mmol/L. However, the formation of amide bonds were not observed until the concentration of lysine reached 20 mmol/L (Fig. S4 in Supporting information). This result may be caused by the distinctive structure of CTP. To overcome this limitation, we first enriched the CTP-binding proteins using streptavidin beads, eliminated non-specific proteins by extensively washing, and then digested the proteins with trypsin before LC-MS/MS analysis (Fig. 2). In this respect, the target proteins would be identified and quantified using the unmodified peptides. In addition, this method also allows the identification of those CTP targets which do not have lysine residues in their CTP-binding pockets.
To exclude the non-specific targets, we employed low (10 µmol/L) and high (100 µmol/L) concentrations of CTP probe, along with SILAC-based quantitative proteomics approach, to identify CTP-binding proteins in human cells. Prior to incubation with the probe, cell lysates were passed through NAP-25 columns to remove the endogenous nucleotides. In forward SILAC experiment, equal amount of light and heavy cell lysates were incubated with low (10 µmol/L) and high (100 µmol/L) concentrations of CTP probe, respectively (Fig. 2a). In reverse SILAC experiment, labeling reaction was operated in an opposite way. Biotin-labeled proteins were enriched by streptavidin beads, digested with trypsin, and analyzed using LC-MS/MS as described above. The peak intensity ratios of heavy and light peptides were used to obtain the relative affinity ratios (RCTP probe 10/1, 100 µmol/L/10 µmol/L) of proteins towards different concentrations of CTP probe.
Similar as the previous quantitative affinity profiling of ATP-binding proteins, specific binding proteins would show RCTP probe 10/1 close to 1, whereas non-specific proteins would display RCTP probe 10/1 ≫ 1 due to concentration-dependent increase in peptides arising from non-specific proteins [18]. To include all the putative CTP-binding proteins, a criterion of RCTP probe 10/1 < 2 was used to select the specific CTP targets. With the criterion, a total of 575 proteins were identified and quantified in at least two SILAC experiments (Fig. 3a and Table S1 in Supporting information). Some known CTP-binding proteins, such as CTP synthase 1 (CTPS1) [19] and heat shock proteins HSP90s [20], were included in the list (RCTP probe 10/1 values of ~1, Fig. 3b and Table S1), demonstrating the feasibility of our strategy in profiling CTP-binding proteins.
To further ascertain whether these proteins bind selectively to CTP, we performed competitive SILAC experiments in which free CTP acted as a competitor to compete with CTP probe (Fig. 2b). Cell lysates were pretreated with free CTP before incubation with CTP affinity probes. In this respect, CTP would occupy the binding pockets of specific targets, so that the labeling efficiency of CTP-binding proteins by CTP affinity probes would decrease. In forward SILAC experiment, the heavy and light cell lysates were incubated with and without free CTP, respectively, whereas reverse SILAC experiment was manipulated oppositely. CTP competitive ratio of protein (RCTP/CTP probe) was used to reflect its relative binding affinity towards CTP probe with vs. without free CTP. To include all the putative CTP targets, a criterion of RCTP/CTP probe < 1 was used to choose the candidate CTP-binding proteins. LC-MS/MS analysis identified and quantified 311 proteins in at least one SILAC experiment, 129 of which display RCTP/CTP probe < 1 (Fig. 3c and Table S2 in Supporting information). For example, when treating the heavy cell lysate using free CTP in forward SILAC experiment, the peak intensities of the heavy-labeled peptides from ACTB are lower than those of the light-labeled peptides without CTP treatment (Fig. 3d). A similar trend was also observed in reverse SILAC experiment (Fig. 3e, Fig. S5 in Supporting information showing the MS/MS).
We analyzed the data from these two sets of SILAC experiments and identified 90 proteins with RCTP probe 10/1 < 2 in probe concentration-dependent SILAC experiments and RCTP/CTP probe < 1 in free CTP competition experiments (Fig. 4a, Table S3 in Supporting information). These proteins contain a small fraction of known CTP targets and a large proportion of newly discovered proteins. We further investigated the biological functions of these proteins by Gene Ontology (GO) analysis using DAVID database. The results showed that these proteins are involved in a variety of cellular processes, such as nucleotide binding, protein folding and cell adhension (Fig. 4b). We ranked these proteins according to their RCTP/CTP probe value (Fig. 4c and Table S3) and found that CTPS1 is among the top on the list, suggesting that this quantitative proteomics strategy could be employed for identifying new CTP-binding proteins.
In summary, we reported here a quantitative proteomics strategy using a biotinylated CTP affinity probe to profile CTP-binding proteins at the entire proteome scale. Previous studies showed that the identification of nucleotide-binding proteins by conventional bottom-up method is very challenging due to the relatively low abundance of nucleotide-binding proteins and the interference from other cellular proteins [11, 12], whereas the method used in this study can effectively capture nucleotide-binding proteins. We successfully synthesized the biotin-tagged CTP affinity probe and used it to enrich CTP-binding proteins by two sets of competitive SILAC experiments. We identified a total of 90 potential CTP-binding proteins which showed high affinity to CTP. In addition, we found that these candidate CTP-binding proteins are involved in multiple cellular pathways. These results deepen our understanding of CTP-binding proteins and lay a foundation for further revealing the biological functions of CTP. Moreover, the analytical approach used in this study could be employed for uncovering CTP-binding proteins in other organisms.
Declaration of competing interest
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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Acknowledgments
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This work was supported by the National Natural Science Foundation of China (Nos. 21807030, 21907028), the Science and Technology Innovation Program of Hunan Province (No. 2019RS2020), Natural Science Foundation of Hunan Province (No. 2020JJ5046), and the Fundamental Research Funds for the Central Universities (Nos. 531118010061, 531118010259).
Supplementary materials
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Supplementary material associated with this article can be found, in the online version, at doi: 10.1016/j.cclet.2021.05.057.
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Year 2021 volume 32 Issue 11
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doi: 10.1016/j.cclet.2021.05.057
  • Receive Date：2021-03-09
  • Online Date：2026-01-04
  • Published：2021-11-15
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  • Received：2021-03-09
  • Revised：2021-04-21
  • Accepted：2021-05-24
Affiliations
    State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Molecular Science and Biomedicine Laboratory, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha 410082, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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Genus		 种数
Number of
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Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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