Home Journals Articles Updates About
CN
image
收藏切换
Generation of (E)-β-trifluoromethyl vinylsulfonohydrazides under photocatalysis and their anti-bacteria activity
收藏切换
PDF
Xinhua Wanga, 1, Wei Zhoub, 1, Wenlin Xiee, Qi Chena, *, Jie Wua, c, d, *
Chinese Chemical Letters | 2023, 34(6) : 107984
Less
收藏切换
Chinese Chemical Letters | 2023, 34(6): 107984
• Communication •
Generation of (E)-β-trifluoromethyl vinylsulfonohydrazides under photocatalysis and their anti-bacteria activity
Full
Xinhua Wanga, 1, Wei Zhoub, 1, Wenlin Xiee, Qi Chena, *, Jie Wua, c, d, *
Affiliations
  • aTaizhou Central Hospital (Taizhou University Hospital) & School of Pharmaceutical and Chemical Engineering, Taizhou University, Taizhou 318000, China
  • bDepartment of Chemistry, Fudan University, Shanghai 200438, China
  • cState Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China
  • dSchool of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China
  • eSchool of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China
Published: 2023-06-15 doi: 10.1016/j.cclet.2022.107984
Outline
收藏切换

Trifluoromethylation/sulfonylation of alkynes from trifluoromethyl thianthrenium triflate and sulfur dioxide under extremely mild reaction conditions provides a facile access to trifluoromethyl-substituted vinyl sulfonohydrazides in moderate to good yields. This multicomponent reaction of trifluoromethyl thianthrenium triflate, alkynes, sulfur dioxide and hydrazines proceeds efficiently under visible light irradiation in the presence of photocatalyst at room temperature with broad substrate scope and excellent functional group compatibility. This reaction is highly stereoselective, and only (E)-isomers are obtained. Additionally, these trifluoromethyl-substituted vinyl sulfonohydrazides are further evaluated for anti-bacteria activity. In vitro activities of these compounds against Staphylococcus aureus (G+) and Escherichia coli (G−) are examined.

Alkyne  /  Hydrazine  /  Photocatalysis  /  Sulfur dioxide  /  Trifluoromethyl thianthrenium triflate
Xinhua Wang, Wei Zhou, Wenlin Xie, Qi Chen, Jie Wu. Generation of (E)-β-trifluoromethyl vinylsulfonohydrazides under photocatalysis and their anti-bacteria activity[J]. Chinese Chemical Letters, 2023 , 34 (6) : 107984 - . DOI: 10.1016/j.cclet.2022.107984
Full Text
Less
It is well established that compounds with substitution of fluorine might have a higher stability against metabolic enzymes and a better membranous permeability [1-10]. Therefore, introducing a trifluoromethyl group into small molecule is highly desirable. Recently, trifluoromethyl thianthrenium triflate (TT-CF3+OTf−) developed by Ritter and co-workers as a novel trifluoromethylating reagent has attracted much attention [11]. This trifluoromethylating reagent can be easily synthesized via a single step from thianthrene and triflic anhydride. So far, the application of TT-CF3+OTf− in electrophilic, radical, and nucleophilic trifluoromethylation reactions has been demonstrated [12,13]. For instance, synthesis of Cα-tetrasubstituted α- and β-amino acid analogues via a radical conjugate addition of a trifluoromethyl thianthrenium salt to Michael acceptors with acetonitrile could be achieved [14]. During the transformation, α-thianthrenium carbonyl species as the equivalent of an α-carbonyl carbocation was the key intermediate. As part of a program for the generation of trifluoromethyl-substituted small molecules for biological evaluations, we are interested in methodologies development by using trifluoromethyl thianthrenium triflate (TT-CF3+OTf−) as the trifluoromethylating reagent.
It is known that natural products and pharmaceuticals containing sulfonyl group exhibit remarkable biological activities [15-20]. For example, vinyl sulfones have been identified as anti-Gram-positive bacteria as SrtA transpeptidase inhibitors [21], anti-parasitic as cysteine protease inhibitors [22], and anti-virus as potent inhibitors of HIV-1 integrase [23]. Additionally, many sulfonyl compounds are marketed drugs. In the past decade, generation of sulfones and sulfonamides by using sulfur dioxide surrogates as the source of sulfonyl group has attracted much attention [24-51]. We also focus on the chemistry of sulfur dioxide insertion for the preparation of diverse sulfonyl compounds from the sulfur dioxide surrogates of DABCO·(SO2)2 and inorganic sulfites [52-66]. Encouraged by the recent advance of trifluoromethyl thianthrenium triflate (TT-CF3+OTf−) [1-13], we envisioned that trifluoromethyl group and sulfonyl group could be incorporated in one molecule by using trifluoromethyl thianthrenium triflate (TT-CF3+OTf−) as the trifluoromethylating reagent and sulfur dioxide as the sulfonyl source. It was reported that compounds containing an α, β-unsaturated vinyl sulfone moiety exhibited modest inhibitory potencies in inflammation as a novel class toward Parkinson's [67] and arthritis [68] disease therapy by depressing the expression of endothelial cells of adhesion molecules. Additionally, antitumor activity of trifluoromethyl-substituted vinyl sulfones was discovered through activation of autophagy by suppression PI3K/Akt/mTOR signal pathway [13]. Therefore, we conceived that a small library of trifluoromethyl-substituted vinyl sulfonyl compounds may be constructed, which is beneficial for their further biological evaluations. Herein, we report the synthesis of trifluoromethyl-substituted vinyl sulfonohydrazides through a four-component reaction of alkynes, trifluoromethyl thianthrenium triflate (TT-CF3+OTf−), DABCO·(SO2)2 and hydrazines under extremely mild conditions. This transformation proceeds smoothly under visible light irradiation in the presence of photocatalyst at room temperature. Additionally, in vitro activities of these trifluoromethyl-substituted vinyl sulfonohydrazides against Staphylococcus aureus (G+) and Escherichia coli (G−) are further evaluated.
To verify the practicability of our hypothesis, commercially available hydrazine was selected as the partner for reaction development. At the outset, a reaction of trifluoromethyl thianthrenium triflate, 4-methylphenylacetylene 1a, DABCO·(SO2)2 and morpholin-4-amine 2a was explored as the model for condition optimization (Table 1). Initially, the reaction was performed at 25 ℃ in MeCN. To our delight, the desired trifluoromethyl-substituted vinyl sulfonohydrazide 3a was generated in 40% yield (Table 1, entry 1). However, the outcome could not be improved when other solvents were used (data not shown in Table 1). The yield was lower when the reaction occurred in the dark (Table 1, entry 2). From this result, we reasoned that the presence of visible light irradiation might facilitate the formation of trifluoromethyl radical [14]. Therefore, we further examined the reaction with the addition of photocatalyst under 36 W blue LEDs irradiation. Interestingly, the desired product 3a was afforded in 92% yield in the presence of Ir(ppy)3 (Table 1, entry 3). No better result was obtained when other photocatalysts were utilized including Ru(bpy)Cl2·6H2O, 4CzIPN, EosinY and Ir[dF(CF3)ppy]2(dtbbpy)PF6 (Table 1, entries 4–7). We further investigated this photocatalytic reaction in various solvents. As shown in Table 1, MeCN was still the best choice in this transformation (entries 8–13). Meanwhile, the structure and configuration of compound 3a was determined as (E)-β-trifluoromethyl vinylsulfonohydrazide by single-crystal X-ray diffraction analysis (CCDC: 2176532). No reaction occurred when amine, TsNHNH2 or PhCONHNH2 was used as a replacement of hydrazine. For the reaction by employing O-phenethylhydroxylamine as the reactant instead of morpholin-4-amine 2a, only a trace amount of product was detected. Inferior results were obtained when the sulfur dioxide surrogate of DABCO·(SO2)2 was changed to NaHSO3 (sodium hydrogen sulfite), potassium metabisulfite or sodium metabisulfite (data not shown in Table 1).
With the above promising result in hand, we subsequently explored the substrate scope of this multicomponent reaction of alkynes, trifluoromethyl thianthrenium triflate, sulfur dioxide and hydrazines under photocatalytic conditions. As shown in Scheme 1, it was found that a wide range of arylalkynes bearing electron-donating and electron-withdrawing substituents in the aromatic ring were workable in this transformation. Notably, some sensitive functional groups (such as amino and ester) were compatible. For instance, reaction of trifluoromethyl thianthrenium triflate (TT-CF3+OTf−), 4-ethynylaniline, DABCO·(SO2)2 and morpholin-4-amine 2a proceeded smoothly, giving rise to the corresponding amino-substituted product 3f in 71% yield. The ester-containing products 3k and 3l could be afforded smoothly as well. Meanwhile, reactions employing other heterocycle-substituted alkynes were explored. Pyridinyl-substituted product 3s and thiophenyl-substituted product 3t were produced in 67% yield, respectively. The excellent functional group tolerance supported the practicality of this reaction. Additionally, reactions of alkyl alkynes, trifluoromethyl thianthrenium triflate (TT-CF3+OTf−), DABCO·(SO2)2 and morpholin-4-amine 2a were examined, leading to the desired products as expected (3u and 3v). Interestingly, trimethylsilylacetylene was a good reactant as well, and the trimethylsilyl-substituted product 3w was provided in 58% yield. We further examined the reaction by using other hydrazines in the reaction of trifluoromethyl thianthrenium triflate, DABCO·(SO2)2 and 4-methoxylphenylacetylene under photocatalytic conditions, and the desired products 3x-3z were generated in good yields. To demonstrate the practicability of this trifluoromethylation/sulfonylation of alkyne, a gram-scale experiment (1.0 mol) was carried out, which provided the corresponding product 3a in 71% yield.
On the basis of previous reports [11], a plausible mechanism is proposed, as shown in Scheme 2. It was reasoned that in the presence of photocatalyst under visible light irradiation, trifluoromethyl radical would be formed from trifluoromethyl thianthrenium triflate (TT-CF3+OTf−) through a single electron transfer. Then, trifluoromethyl radical would be trapped by alkyne 1 giving rise to vinyl radical intermediate A, which would react with sulfur dioxide leading to sulfonyl radical intermediate B. In the meantime, hydrazine 2 would convert to cation radical intermediate C with the assistance of photocatalyst. After deprotonation, radical D would be produced, which would subsequently combine with sulfonyl radical intermediate B to provide trifluoromethyl-substituted vinyl sulfonohydrazide 3.
All the prepared trifluoromethyl-substituted vinyl sulfonohydrazide derivatives were evaluated for their antibacterial activities in vitro against Gram-positive bacterium (S. aureus) and Gram-negative bacterium (E. coli), using the standard two folds serial dilution method in 10 × 150 mm glass tubes. Initially, the compounds were dissolved in dimethyl sulfoxide (DMSO) to prepare the stock solutions (40 mmol/L), then the tested compounds were prepared in LB broth (Tryptone 10 g/L, Yeast extract 5 g/L, NaCl 10 g/L, pH 7.4) to obtain the required concentration of 40 µmol/L (2 mL) in tubes. The bacterial suspension was adjusted with sterile saline to a concentration of 1 × 105 CFU and 100 µL aliquots of the test organisms were added to the appropriate tubes. Inoculated tubes were incubated aerobically at 37 ℃, 150 r.p.m. (round per minute) for 18 h. The growth inhibition was determined to be the suppression of optical density at 620 nm.
The results in Table 2 revealed that all of the trifluoromethyl-substituted vinyl sulfonohydrazides displayed no activities against the tested strains. But interestingly, compound 3y effectively inhibited the growth of S. aureus and also displayed weak activity against E. coli, and the MIC (the lowest concentration that inhibit visible growth) against S. aureus was furtherly detected to be 10 µmol/L, which indicated that the structure of sulfonyl hydrazine might be a novel potential antibacterial pharmacophore, especially for Gram positive bacteria.
In conclusion, we have developed an efficient trifluoromethylation/sulfonylation of alkynes from trifluoromethyl thianthrenium triflate and sulfur dioxide under extremely mild reaction conditions, providing a facile access to trifluoromethyl-substituted vinyl sulfonohydrazides in moderate to good yields. This multicomponent reaction of trifluoromethyl thianthrenium triflate, alkynes, sulfur dioxide and hydrazines proceeds efficiently under visible light irradiation in the presence of photocatalyst at room temperature with broad substrate scope and excellent functional group compatibility. It is noteworthy that this reaction is highly stereoselective, since only (E)-isomer is obtained. Additionally, these trifluoromethyl-substituted vinyl sulfonohydrazides are further evaluated for anti-bacteria activity. In vitro activities of these compounds against S. aureus (G+) and E. coli (G−) are examined.
Declaration of competing interest
Less
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Acknowledgments
Less
Financial support from National Natural Science Foundation of China (No. 21871053), the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang (No. 2019R01005) and the Open Research Fund of School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD04) is gratefully acknowledged.
Supplementary materials
Less
Supplementary material associated with this article can be found, in the online version, at doi:10.1016/j.cclet.2022.107984.
References
Less
[1]
H. Mei, J. Han, S. Fustero, et al., Chem. Eur. J. 25 (2019) 11797–11819.
[2]
H. Mei, A.M. Remete, Y. Zou, et al., Chin. Chem. Lett. 31 (2020) 2401–2413.
[3]
J. Han, L. Kiss, H. Mei, et al., Chem. Rev. 121 (2021) 4678–4742.
[4]
Y. Yu, A. Liu, G. Dhawan, et al., Chin. Chem. Lett. 32 (2021) 3342–3354.
[5]
J. He, Z. Li, G. Dhawan, et al., Chin. Chem. Lett. 34 (2023) 107578.
[6]
Q. Wang, J. Han, A. Sorochinsky, et al., Pharmaceuticals 15 (2022) 999.
[7]
S. Purser, P.R. Moore, S. Swallow, V. Gouverneur, Chem. Soc. Rev. 37 (2008) 320.
[8]
W.K. Hagmann, J. Med. Chem. 51 (2008) 4359.
[9]
J. Wang, M. SanchezRosello, J.L. Aceña, et al., Chem. Rev. 114 (2014) 2432.
[10]
Y. Zhou, J. Wang, Z. Gu, et al., Chem. Rev. 116 (2016) 422.
[11]
H. Jia, A.P. Häring, F. Berger, L. Zhang, T. Ritter, J. Am. Chem. Soc. 143 (2021) 7623–7628.
[12]
Y. Li, X. Liang, K. Niu, et al., Org. Lett. 24 (2022) 5918–5923.
[13]
J. Zhang, X. Wang, Q. Chen, et al., Eur. J. Med. Chem. 232 (2022) 114197.
[14]
H. Jia, T. Ritter, Angew. Chem. Int. Ed. 61 (2022) e202208978.
[15]
D.C. Meadows, T.B. Mathews, T.W. North, et al., J. Med. Chem. 48 (2005) 4526–4534.
[16]
R. Ettari, E. Nizi, M.E. Di Francesco, et al., J. Med. Chem. 51 (2008) 988–996.
[17]
N.K. Konduru, S. Dey, M. Sajid, M. Owais, N. Ahmed, Eur. J. Med. Chem. 59 (2013) 23–30.
[18]
H. Sasabe, Y. Seino, M. Kimura, J. Kido, Chem. Mater. 24 (2012) 1404–1406.
[19]
G. Turkoglu, M.E. Cinar, T. Ozturk, Top. Curr. Chem. 375 (2017) 84.
[20]
J.T. Petroff II, K.N. Skubic, C.K. Arnatt, R.D. McCulla, J. Org. Chem. 83 (2018) 14063–14068.
[21]
B.A. Frankel, M. Bentley, R.G. Kruger, D.G. Mccafferty, J. Am. Chem. Soc. 126 (2004) 3404–3405.
[22]
I.D. Kerr, J.H. Lee, C.J. Farady, et al., J. Biol. Chem. 284 (2009) 25697–25703.
[23]
D.C. Meadows, J. Gervay-Hague, Med. Res. Rev. 26 (2006) 793–814.
[24]
D. Zheng, J. Wu, Sulfur Dioxide Insertion Reactions for Organic Synthesis, Nature Springer, Berlin, 2017.
[25]
G. Qiu, K. Zhou, L. Gao, J. Wu, Org. Chem. Front. 5 (2018) 691–705.
[26]
K. Hofman, N. Liu, G. Manolikakes, Chem. Eur. J. 24 (2018) 11852–11863.
[27]
G. Qiu, L. Lai, J. Cheng, J. Wu, Chem. Commun. 54 (2018) 10405–10414.
[28]
G. Qiu, K. Zhou, J. Wu, Chem. Commun. 54 (2018) 12561–12569.
[29]
S. Ye, G. Qiu, J. Wu, Chem. Commun. 55 (2019) 1013–1019.
[30]
S. Ye, M. Yang, J. Wu, Chem. Commun. 56 (2020) 4145–4155.
[31]
D. Zeng, M. Wang, W.P. Deng, X. Jiang, Org. Chem. Front. 7 (2020) 3956–3966.
[32]
F.S. He, M. Yang, S. Ye, J. Wu, Chin. Chem. Lett. 32 (2021) 461–464.
[33]
S.P. Blum, K. Hofman, G. Manolikakes, S.R. Waldvogel, Chem. Commun. 57 (2021) 8236–8249.
[34]
X. Jia, S. Kramer, T. Skrydstrup, Z. Lian, Angew. Chem. Int. Ed. 60 (2021) 7353–7359.
[35]
Y. Li, S. Chen, M. Wang, X. Jiang, Angew. Chem. Int. Ed. 59 (2020) 8907–8911.
[36]
X. Chen, B. Tang, C. He, et al., Chem. Commun. 56 (2020) 13653–13656.
[37]
F. Herrera, A. Luna, P. Almendros, Org. Lett. 22 (2020) 9490–9494.
[38]
S.P. Blum, T. Karakaya, D. Schollmeyer, A. Klapars, S.R. Waldvogel, Angew. Chem. Int. Ed. 60 (2021) 5056–5062.
[39]
Y. Lv, J. Luo, Y. Ma, Q. Dong, L. He, Org. Chem. Front. 8 (2021) 2461–2467.
[40]
X. Jia, C. Huang, X. Zhang, Z. Lian, Org. Chem. Front. 8 (2021) 5310–5315.
[41]
M.A. Idris, S. Lee, Org. Lett. 23 (2021) 4516–4520.
[42]
Z. Chen, H. Zhang, S. Zhou, X. Cui, Org. Lett. 23 (2021) 7992–7995.
[43]
T.S.B. Lou, Y. Kawamata, T. Ewing, et al., Angew. Chem. Int. Ed. 61 (2022) e202208080.
[44]
L. Chen, X. Zhang, M. Zhou, L. Shen, S. Kramer, Z. Lian, ACS Catal. 12 (2022) 10764–10770.
[45]
Z. Chen, H. Zhang, S. Zhou, X. Cui, Org. Chem. Front. 9 (2022) 364–369.
[46]
R. Ding, Y. Deng, S. Luo, et al., Org. Chem. Front. 9 (2022) 2228–2233.
[47]
Y. Li, X. Zhang, Z. Lian, Org. Chem. Front. 9 (2022) 5141–5146.
[48]
H. Konishi, R. Fujita, M. Yamaguchi, K. Manabe, Org. Lett. 24 (2022) 3663–3667.
[49]
Y. Meng, M. Wang, X. Jiang, Angew. Chem. Int. Ed. 59 (2020) 1346–1353.
[50]
S. Cao, W. Hong, Z. Ye, L. Gong, Nat. Commun. 12 (2021) 2377.
[51]
T. Zhong, J. Yi, Z. Chen, et al., Chem. Sci. 12 (2021) 9359–9365.
[52]
C. Zhang, Z. Tang, Y. Qiu, et al., Chem. Catal. 2 (2022) 164–177.
[53]
D. Zheng, J. Yu, J. Wu, Angew. Chem. Int. Ed. 55 (2016) 11925–11929.
[54]
F. -. S. He, C. Zhang, L. Lou, et al., Chem. Sci. 13 (2022) 8834–8839.
[55]
X. Gong, M. Yang, J.B. Liu, F.S. He, J. Wu, Org. Chem. Front. 7 (2020) 938–943.
[56]
Y. Liu, X. Zhang, J. Lv, et al., Org. Chem. Front. 9 (2022) 450–455.
[57]
J. Zhang, J. Wu, X. Chang, et al., Org. Chem. Front. 9 (2022) 917–922.
[58]
F.S. He, L. Su, F. Yu, Z. Tang, J. Wu, Org. Chem. Front. 9 (2022) 1937–1942.
[59]
X. Wang, J. Zhang, Q. Chen, W. Zhou, J. Wu, Chin. Chem. Lett. 33 (2022) 4860–8464.
[60]
X. Wang, M. Yang, S. Ye, Y. Kuang, J. Wu, Chem. Sci. 12 (2021) 6437–6441.
[61]
F.S. He, P. Bao, F. Yu, L. Zeng, et al., Org. Lett. 23 (2021) 7472–7476.
[62]
F.S. He, M. Zhang, X. Luo, J. Wu, Org. Chem. Front. 8 (2021) 3746–3751.
[63]
F. He, Y. Yao, Z. Tang, W. Xie, J. Wu, Org. Chem. Front. 8 (2021) 6119–6124.
[64]
K. Zhou, J. Huang, J. Wu, Q. Qiu, Chin. Chem. Lett. 32 (2021) 37–39.
[65]
F.S. He, M. Yang, S. Ye, J. Wu, Chin. Chem. Lett. 32 (2021) 461–464.
[66]
F. Liu, J. Huang, X. Wu, et al., J. Org. Chem. 87 (2022) 6137–6145.
[67]
S. Woo, J. Kim, M. Moon, et al., J. Med. Chem. 57 (2014) 1473–1487.
[68]
L. Ni, S. Zheng, P.K. Somers, et al., Bioorg. Med. Chem. Lett. 13 (2003) 745–748.
Appendix
Less
Year 2023 volume 34 Issue 6
PDF
58
32
Cite this Article
BibTeX
Article Info
doi: 10.1016/j.cclet.2022.107984
  • Receive Date：2022-09-23
  • Online Date：2025-11-21
  • Published：2023-06-15
Article Data
Affiliations
History
  • Received：2022-09-23
  • Revised：2022-10-26
  • Accepted：2022-11-03
Affiliations
    aTaizhou Central Hospital (Taizhou University Hospital) & School of Pharmaceutical and Chemical Engineering, Taizhou University, Taizhou 318000, China
    bDepartment of Chemistry, Fudan University, Shanghai 200438, China
    cState Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China
    dSchool of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China
    eSchool of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China
References
Share
https://castjournals.cast.org.cn/joweb/ccl/EN/10.1016/j.cclet.2022.107984
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House