Home Journals Articles Updates About
CN
image
收藏切换
FRET-based two-photon benzo[a] phenothiazinium photosensitizer for fluorescence imaging-guided photodynamic therapy
收藏切换
PDF
Yiling Lia, 1, Zekun Gaob, 1, Xiuxiu Yuea, Minhuan Lana, *, Xiuli Zhengb, *, Benhua Wanga, Shuang Zhaoc, Xiangzhi Songa, *
Chinese Chemical Letters | 2024, 35(7) : 109133
Less
收藏切换
Chinese Chemical Letters | 2024, 35(7): 109133
•   •
FRET-based two-photon benzo[a] phenothiazinium photosensitizer for fluorescence imaging-guided photodynamic therapy
Full
Yiling Lia, 1, Zekun Gaob, 1, Xiuxiu Yuea, Minhuan Lana, *, Xiuli Zhengb, *, Benhua Wanga, Shuang Zhaoc, Xiangzhi Songa, *
Affiliations
  • a College of Chemistry & Chemical Engineering, Central South University, Changsha 410083, China
  • b Key Laboratory of Photochemical Conversion and Optoelectronic Materials and City U-CAS Joint Laboratory of Functional Materials and Devices, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
  • c Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410083, China
Published: 2024-07-15 doi: 10.1016/j.cclet.2023.109133
Outline
收藏切换

To overcome the conflict between the long-wavelength excitation and high singlet oxygen quantum yield of photosensitizers, we conjugated a two-photon fluorophore, tetrahydroquinoxaline coumarin (TQ), and an efficient photodynamic therapeutic agent, benzo[a]phenothiazinium (NBS-NH2), through a hexamethylene linker to build a two-photon photosensitizer, TQ-NBS. In TQ-NBS, TQ served as an energy donor and NBS-NH2 acted as an energy acceptor; and TQ-NBS was a Förster resonance energy transfer (FRET) cassette with a 92.8% efficiency. The large two-photon absorption cross-section of TQ allowed photosensitizer TQ-NBS to work in a 900 nm two-photon excitation (TPE) mode, which greatly benefited the deep tissue penetration in PDT treatment. Meanwhile, the excellent phototoxicity and near-infrared fluorescence of NBS-NH2 was kept in TQ-NBS under a TPE mode via a FRET process. Photosensitizer TQ-NBS exhibited a high phototoxic efficacy in living cells and tumor-bearing mice.

Photodynamic therapy  /  Two-photon excitation  /  FRET-based photosensitizer  /  Singlet oxygen  /  Near-infrared fluorescence
Yiling Li, Zekun Gao, Xiuxiu Yue, Minhuan Lan, Xiuli Zheng, Benhua Wang, Shuang Zhao, Xiangzhi Song. FRET-based two-photon benzo[a] phenothiazinium photosensitizer for fluorescence imaging-guided photodynamic therapy[J]. Chinese Chemical Letters, 2024 , 35 (7) : 109133 - . DOI: 10.1016/j.cclet.2023.109133
Full Text
Less
Compared with traditional clinical treatments (e.g., chemotherapy [1], radiotherapy [2], and surgery [3]), photodynamic therapy (PDT) has emerged as a promising cancer treatment with the advantages of high selectivity, no drug resistance, and negligible invasiveness [4–7]. In PDT treatment, photosensitizers absorb light to be excited to triplet state; and a triplet-triplet energy transfer between the excited molecules of the photosensitizers and the O2 molecules generates singlet oxygen (1O2), which are highly destructive to cancer cells [8–10]. Therefore, under light irradiation and in the presence of O2, photosensitizers display efficient phototoxicity to cancer cells and tumors with high selectivity [11,12]. Since light is necessary for PDT treatment, long-wavelength photons are desirable because of their deep tissue penetration [13,14]. Consequently, researchers have made a lot of efforts to develop photosensitizers having long-wavelength absorptions in optical window (600–900 nm). According to Fermi's golden rule, however, there is a limit for 1O2 quantum yield of photosensitizers with an absorption mainly in near-infrared (NIR) region [15]. The longer the absorption wavelength is, the lower the 1O2 quantum yield of photosensitizers is [9,16]. Moreover, NIR photosensitizers usually have complicated chemical structures and poor photostability [17,18].
Compared with one-photon excitation (OPE), two-photon excitation (TPE) technology employs with two simultaneous photons with longer wavelength to excite the fluorophore, and thereby having deeper tissue penetration and higher imaging resolution [19–21]. Recently, TPE technology has been becoming attractive in PDT because deep red or NIR pulsed laser can be used to illuminate photosensitizers to produce 1O2 to induce cell death [22]. Besides, TPE has a high spatial resolution of fluorescence imaging and minimal photodamage to the surrounding healthy tissue [23–26]. However, intrinsic TPE photosensitizers usually have small two-photon absorption cross-sections, which results in the low 1O2 generation efficiency [27,28]. In order to solve the above issues, Förster resonance energy transfer (FRET) mechanism has been used to design TPE-PDT by conjugating a photosensitizer with an energy donor having large two-photon absorption cross-sections such as semiconductor quantum dots [29], carbon dots [30,31], and polymers [32]. However, these complex TPE-PDT agents are subjected to uncontrollable surface modification, biosafety concern, or poor water solubility [33–36]. Therefore, developing small organic molecular TPE photosensitizers with good biocompatibility through FRET mechanism is very necessary.
Benzo[a]phenothiazinium dyes (NBS) are effective photosensitizers for PDT treatment because they have an intense red absorption and a NIR fluorescence, efficient 1O2 generation ability, and good biocompatibility [37–39]. Though the absorption of NBS-NH2 (λmax = 655 nm) is in the optical window, the penetration ability of the excitation light limits its effectiveness in the treatment of tumors with large size or in deep position to some extent. Recently, we reported a tetrahydroquinoxaline coumarin dye (TQ) [40], which has a large two-photon absorption cross-section over 100 GM and an emission with λmax = 585 nm (Fig. 1a). Considering the large spectral overlapping between the absorption of NBS-NH2 and the emission of TQ, we expected to build an efficient FRET cassette with NBS-NH2 and TQ in which TQ acts as the energy donor and NBS-NH2 servers as the energy acceptor. Therefore, we might take the respective advantage of NBS-NH2 and TQ to construct a novel FRET-based TPE photosensitizer (TQ-NBS). As demonstrated in Scheme 1, TQ and NBS-NH2 were conjugated through a hexamethylene linker to give TQ-NBS. Under a two-photon excitation, TQ-NBS can simultaneously generate 1O2 and NIR fluorescence, achieving two-photon imaging-guided PDT for cancer cells.
The preparation of TQ-NBS was depicted in Scheme S1 (Supporting information). The chemical structure of TQ-NBS was characterized by nuclear magnetic resonance (NMR) and high-resolution mass spectral analysis. Next, we investigated the optical properties of TQ-NBS. As shown in Fig. 1b, TQ-NBS showed two intensive absorption bands centered at 455 and 655 nm, assigned to TQ and NBS, respectively. Meanwhile, TQ-NBS exhibited two fluorescence peaks at 555 and 705 nm under OPE, indicating the occurrence of the FRET process. The FRET efficiency in TQ-NBS was determined to be 92.8%, which was much higher than that of most of the previously reported FRET-based photosensitizers [12,41-43]. Moreover, we used TPE to study the optical properties of TQ-NBS. As shown in Fig. 1b, two fluorescence peaks at 545 and 700 nm were observed under 900 nm pulsed laser excitation, clearly proving the existence of the FRET process in TQ-NBS.
The chemical stability of TQ-NBS was investigated in the presence of different biologically relevant analytes. As shown in Fig. S1 (Supporting information), the fluorescence intensity at 705 nm of TQ-NBS hardly changed with the addition of these analytes, revealing that TQ-NBS was chemically stable. Next, we used TQ-NBS to incubate living 4T1 cells and investigate its fluorescence imaging performance in vitro. As indicated in Fig. 1c, strong fluorescence signals in both green and red channels were seen from inside cells when illuminated under a 488 nm laser (OPE). As expected, cells under a 900 nm laser irradiation (TPE) also gave strong green and red fluorescence signals (Fig. 1d). It was seen that the TPE imaging resolution was much higher than the OPE one because TPE can minimize the light scattering and reduce endogenous absorption of biological tissues [26,44].
To evaluate the 1O2 generation ability of TQ-NBS, 9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA) was used as the 1O2 indicator. First, a 480 nm light-emitting diode (LED) was used as a light source to illuminate the mixture of TQ-NBS and ABDA. Under the light irradiation, the absorbance of ABDA sharply decreased after 8 min irradiation, suggesting TQ-NBS could produce 1O2 via the FRET process (Fig. 1e and Fig. S2 in Supporting information). The 1O2 quantum yield of TQ-NBS was determined to be 7.5%. To verify the 1O2 generation capability of TQ-NBS under TPE, electron spin resonance (ESR) spectroscopy was performed using 2,2,6,6-tetramethylpiperidine (TEMP) as the spin trapping agent for 1O2. As shown in Fig. 1f, the ESR signals barely changed in the presence or absence of TQ-NBS without light illumination. Under TPE illumination (900 nm pulsed laser), in contrast, the signals of characteristic paramagnetic adducts were clearly observed, indicating the generation of 1O2.
Furthermore, the 1O2 generation capability of TQ-NBS in living cells was evaluated using 2,7-dichlorofluorescein diacetate (DCFH-DA) as an indicator. A 480 nm LED was used as the light sources. 4T1 cells were incubated with TQ-NBS and DCFH-DA for 30 min. Meanwhile, cells were incubated with phosphate buffered saline (PBS) and DCFH-DA as the control. As shown in Fig. S3 (Supporting information), bright green fluorescence was observed from TQ-NBS-pretreated cells after 20 min light illumination. Without light illumination, however, no obvious fluorescence was detected from TQ-NBS-pretreated cells. For the PBS-treated control group, cells gave off no fluorescence no matter there was a light illumination or not.
Next, calcein AM/propidium iodide (AM/PI) co-staining assay was carried out to evaluate the photocytotoxicity of TQ-NBS. As shown in Fig. 2a, the irradiation under a 480 nm LED on TQ-NBS-treated cells resulted in strong fluorescence in the red channel and reduced fluorescence in the green channel. In the absence of TQ-NBS, the cells only exhibited strong fluorescence in the green channel. We were pleased that the irradiation under a 900 nm pulsed laser also gave the same results. These results proved that TQ-NBS could exhibit high phototoxicity under both OPE and TPE modes. Additionally, the scratch assay was performed on 4T1 cells to further visualize the PDT effect of TQ-NBS. As shown in Fig. 2b, the wound gap became wider after 24 h when TQ-NBS-treated cells were irradiated under a LED light. Regarding the PBS-treated control group, the wound gap was narrowed after 24 h. The wound healing experiments indicated that TQ-NBS had excellent PDT effect on inhibiting cell migration. The cytotoxicity of TQ-NBS was further determined using the MTT assay. As shown in Fig. 2c, the cytotoxicity of TQ-NBS was negligible in the dark. Under a light irradiation for 20 min, TQ-NBS showed strong dose-dependent phototoxicity and the cell viability was less than 20% when cells were treated with 15 µmol/L TQ-NBS.
To confirm whether the PDT effect of TQ-NBS arises from the generated 1O2, we used annexin V-fluorescein isothiocyanate (FITC) and PI as indicators to determine the cell death pathways [45]. As shown in Fig. 3, TQ-NBS-incubated cells exhibited strong fluorescence in both green and red channels after the light irradiation, indicating the cell apoptosis and necrosis [46]. It is known that vitamin C can effectively consume 1O2 [47]. Then, we used vitamin C to incubate TQ-NBS-treated cells and later illuminated cells with the LED light. It was found that the fluorescence in both green and red channels was dramatically weakened. These results strongly supported the fact that the apoptosis of TQ-NBS-treated cells was caused by 1O2.
The in vivo PDT efficiency of TQ-NBS was investigated in 4T1 tumor-bearing mice. All related animal experiments were performed according to guidelines approved by the Ethics Committee of Hunan Normal University. As shown in Fig. 4a, PBS-treated tumors grew very fast after 14 days and the light irradiation had negligible effect on the tumor size. Without light irradiation, TQ-NBS-treated tumors still displayed a fast growth. In a sharp contrast, the growth of TQ-NBS-treated tumors was significantly inhibited under a light irradiation. Furthermore, the body weight of tumor-bearing mice did show negligible changes, and no distinct damage and inflammatory lesions were observed in the central organs after 14 days of treatment (Figs. 4b and c; Fig. S4 in Supporting information). These in vivo experiments suggested that TQ-NBS exhibited good biocompatibility and effective therapeutic effects in the tumor-bearing mice.
In conclusion, a small organic molecular two-photon photosensitizer, TQ-NBS, was developed based on FRET mechanism. Under TPE light irradiation, TQ-NBS displayed NIR fluorescence and good PDT efficacy in living cells. The high penetration ability of TPE and the high FRET efficiency make photosensitizer TQ-NBS a good photosensitizer for the potential deep cancer treatment. This work also provides an inspiration for the development of new TPE photosensitizers in the future.
Declaration of competing interest
Less
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Acknowledgments
Less
This work was supported by National Key Research and Development Program of China (No. 2022YFA1207600) and National Natural Science Foundation of China (Nos. 22178395, 62175262 and 62005294).
Supplementary materials
Less
Supplementary material associated with this article can be found, in the online version, at doi:10.1016/j.cclet.2023.109133.
References
Less
[1]
S. Qin, Y. Cheng, Q. Lei, et al., Biomaterials 171 (2018) 178–197.
[2]
Z. Liu, Z. Xie, W. Li, et al., J. Nanobiotechnol. 19 (2021) 160.
[3]
A. Kudo, M. Shinoda, S. Ariizumi, et al., J. Cancer Res. Clin. Oncol. 146 (2020) 2949–2956.
[4]
S. Houthoofd, M. Vuylsteke, S. Mordon, I. Fourneau, Photodiagn. Photodyn. Ther. 29 (2020) 101568.
[5]
J. Karges, Angew. Chem. Int. Ed. 61 (2022) e202112236.
[6]
S. Liao, M. Cai, R. Zhu, et al., Mol. Pharm. 20 (2023) 875–885.
[7]
F. Xu, H. Li, Q. Yao, et al., Chem. Sci. 10 (2019) 10586–10594.
[8]
F. Bolze, S. Jenni, A. Sour, V. Heitz, Chem. Commun. 53 (2017) 12857–12877.
[9]
G. Feng, G. Zhang, D. Ding, Chem. Soc. Rev. 49 (2020) 8179–8234.
[10]
E. Pang, S. Zhao, B. Wang, et al., Coord. Chem. Rev. 472 (2022) 214780.
[11]
D. Chen, Q. Xu, W. Wang, et al., Small 17 (2021) 2006742.
[12]
L. Shi, P. Zhang, X. Liu, et al., Adv. Mater. 34 (2022) 2206659.
[13]
X. Zhao, Y. Yang, Y. Yu, et al., Chem. Commun. 55 (2019) 13542–13545.
[14]
B. Zheng, D. Zhong, T. Xie, et al., Chem 7 (2021) 1615–1625.
[15]
M.A. Manae, A. Hazra, Phys. Chem. Chem. Phys. 24 (2022) 13266–13274.
[16]
K. Hayashi, M. Nakamura, H. Miki, et al., Adv. Funct. Mater. 24 (2014) 503–513.
[17]
I.W. Badon, C. Kim, J.M. Lim, et al., J. Mater. Chem. B 10 (2022) 1196–1209.
[18]
B. Yin, Q. Qin, Z. Li, et al., Nano Today 45 (2022) 101550.
[19]
X. Zhang, F. Yang, T. Ren, et al., Chin. Chem. Lett. 34 (2023) 107835.
[20]
X. He, B. Situ, M. Gao, et al., Small 15 (2019) 1905080.
[21]
D. Chen, W. Qin, H. Fang, et al., Chin. Chem. Lett. 30 (2019) 1738–1744.
[22]
S. Wang, H. Chen, J. Liu, et al., Adv. Funct. Mater. 30 (2020) 2002546.
[23]
B. Gu, W. Wu, G. Xu, et al., Adv. Mater. 29 (2017) 1701076.
[24]
Y. Yang, H. Zhong, B. Wang, et al., Chin. Chem. Lett. 34 (2023) 107674.
[25]
T. Wu, X. Lu, Z. Yu, et al., J. Mater. Chem. B 11 (2023) 1213–1221.
[26]
Y. Zhou, D. Zhang, G. He, et al., J. Mater. Chem. B 9 (2021) 1009–1017.
[27]
M. Galland, T. Le Bahers, A. Banyasz, et al., Chem. Eur. J. 25 (2019) 9026–9034.
[28]
W.R. Dichtel, J.M. Serin, C. Edder, et al., J. Am. Chem. Soc. 126 (2004) 5380–5381.
[29]
J. Sun, Q. Xin, Y. Yang, et al., Chem. Commun. 54 (2018) 715–718.
[30]
N. Naskar, W. Liu, H. Qi, et al., ACS Appl. Mater. Interfaces 14 (2022) 48327–48340.
[31]
K. Yang, C. Wang, C. Liu, et al., J. Mater. Sci. 54 (2019) 3383–3391.
[32]
C. Wu, Q. Xu, Macromol. Rapid Commun. 30 (2009) 504–508.
[33]
R.L. Pinals, L. Chio, F. Ledesma, M.P. Landry, Analyst 145 (2020) 5090–5112.
[34]
W. Qiu, M. Liang, Y. Gao, et al., Theranostics 11 (2021) 9652–9666.
[35]
R. Timor, H. Weitman, N. Waiskopf, et al., ACS Appl. Mater. Interfaces 7 (2015) 21107–21114.
[36]
Z. Xu, L. Mei, Y. Shi, et al., Biomacromolecules 23 (2022) 2778–2784.
[37]
J.W. Foley, X. Song, T.N. Demidova, et al., J. Med. Chem. 49 (2006) 5291–5299.
[38]
M. Li, J. Xia, R. Tian, et al., J. Am. Chem. Soc. 140 (2018) 14851–14859.
[39]
M. Li, T. Xiong, J. Du, et al., J. Am. Chem. Soc. 141 (2019) 2695–2702.
[40]
L. He, H. Xiong, B. Wang, et al., Anal. Chem. 92 (2020) 11029–11034.
[41]
H. Cao, L. Wang, Y. Yang, et al., Angew. Chem. Int. Ed. 57 (2018) 7759–7763.
[42]
J. Liu, L. Wang, R. Shen, et al., Spectrochim. Acta Part A 274 (2022) 121083.
[43]
J. Wang, J. Li, Z. Yu, et al., Anal. Chem. 94 (2022) 14029–14037.
[44]
V. Juvekar, H.W. Lee, D.J. Lee, H.M. Kim, Trends Anal. Chem. 157 (2022) 116787.
[45]
Y. Zhao, Y. Liu, W. Wang, et al., J. Funct. Foods 49 (2018) 412–423.
[46]
S. Banesh, V. Trivedi, Mol. Biotechnol. 63 (2021) 992–1003.
[47]
A. Zhitkovich, Chem. Res. Toxicol. 33 (2020) 2515–2526.
Appendix
Less
Year 2024 volume 35 Issue 7
PDF
65
37
Cite this Article
BibTeX
Article Info
doi: 10.1016/j.cclet.2023.109133
  • Receive Date：2023-06-23
  • Online Date：2025-11-21
  • Published：2024-07-15
Article Data
Affiliations
History
  • Received：2023-06-23
  • Revised：2023-09-12
  • Accepted：2023-09-20
Affiliations
    a College of Chemistry & Chemical Engineering, Central South University, Changsha 410083, China
    b Key Laboratory of Photochemical Conversion and Optoelectronic Materials and City U-CAS Joint Laboratory of Functional Materials and Devices, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
    c Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410083, China
References
Share
https://castjournals.cast.org.cn/joweb/ccl/EN/10.1016/j.cclet.2023.109133
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House