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A near-infrared naphthalocyanine photosensitizer with superior light absorption and renal clearance for type-Ⅰ photodynamic and photothermal combination therapy
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Li Li, Jiale Wen, Xiaojun Zhang, Shuwen Fu, Zixuan Chen, Kai Huang, Luyue Fang, Tinghe Zhao, Peipei Zhang, Xingshu Li*
Chinese Chemical Letters | 2025, 36(6) : 110290
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Chinese Chemical Letters | 2025, 36(6): 110290
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A near-infrared naphthalocyanine photosensitizer with superior light absorption and renal clearance for type-Ⅰ photodynamic and photothermal combination therapy
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Li Li, Jiale Wen, Xiaojun Zhang, Shuwen Fu, Zixuan Chen, Kai Huang, Luyue Fang, Tinghe Zhao, Peipei Zhang, Xingshu Li*
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  • Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, College of Chemistry, Fuzhou University, Fuzhou 350108, China
Published: 2025-06-15 doi: 10.1016/j.cclet.2024.110290
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Currently, it is still a challenge to develop an organic photosensitizer (PS) with outstanding near-infrared absorption, low O2 dependence, precise tumor targeting and rapid clearance through the kidney to improve the overall outcome of phototherapy. In this study, we have designed an organic PS (NcPB) with an excellent near-infrared light absorption through a refined molecular strategy. Meanwhile, NcPB was assembled into nanoparticles with different sizes (NanoNcPB-1 and NanoNcPB-0) by a supramolecular modulation strategy. As the results, the nanoparticle with an ultra-small size (NanoNcPB-1) generated a large number of superoxide anion (O2•−) in a low-O2-dependent manner and release plenty of heat. Furthermore, the results of in vivo experiments demonstrated that NanoNcPB-1 actively accumulated in tumor tissues and showed a 92% tumor inhibition after photodynamic and photothermal combination therapy. More importantly, NanoNcPB-1 could be rapidly cleared from the body of mice via the renal pathway, which alleviates potential side effects of prolonged retention of PS in the circulation.

Naphthalocyanine  /  Near-infrared absorption  /  Renal clearance  /  Superoxide anion  /  Type-Ⅰ photosensitizer
Li Li, Jiale Wen, Xiaojun Zhang, Shuwen Fu, Zixuan Chen, Kai Huang, Luyue Fang, Tinghe Zhao, Peipei Zhang, Xingshu Li. A near-infrared naphthalocyanine photosensitizer with superior light absorption and renal clearance for type-Ⅰ photodynamic and photothermal combination therapy[J]. Chinese Chemical Letters, 2025 , 36 (6) : 110290 - . DOI: 10.1016/j.cclet.2024.110290
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Cancer brings about severe and urgent threats to human beings [1, 2]. Unlike conventional treatment modalities, phototherapy is considered a promising strategy for cancer treatment due to its high selectivity, low side effects and non-invasiveness [3]. Photodynamic therapy (PDT) and photothermal therapy (PTT) are two forms of phototherapy that use light-activated photosensitizers (PSs) to produce reactive oxygen species (ROS) or heat that cause cell death [4, 5]. In general, the combination treatment of PDT and PTT can compensate for the shortcomings of each therapy and maximize its advantages. For example, in addition to acting as a trigger for the death of cancer cells, the thermal effect of PTT stimulates blood circulation within the tumor, which increases the amount of O2 delivered to the hypoxic tumor tissues [6-9]. For another, PDT-produced ROS can also enhance PTT efficiency by inhibiting hot shock proteins [10-12].
To date, the type-Ⅱ mechanism has been adopted for the majority of PDT-related PSs linked to clinical or preclinical applications [13, 14]. In this process, singlet oxygen (1O2) is produced by the PSs in their triplet excited state (3PS*) transferring energy to the surrounding O2 [15-17]. The restricted O2 supply significantly reduces the effectiveness of these PSs for PDT because of the aberrant proliferation of tumor cells [18-22]. Conversely, in the type Ⅰ mechanism of PDT, ROS are generated through a hydrogen or electron transfer process between 3PS* and the biological substrate, greatly reducing the degree of O2 requirement [23-25]. The PSs that undergo the type-Ⅰ pathway are getting more and more attention [26-28]. However, the absorption wavelength of PSs is mainly focused on the ultraviolet and visible region, which limits their clinical translation due to the poor tissue penetration depth of the light [29, 30]. In contrast, the near-infrared (NIR) light has better penetration depth and biocompatibility with the living organisms, minimizing the scattering and attenuation of the irradiation in the tissues [31-34]. Therefore, NIR PS which following the type-Ⅰ mechanism is beneficial for improving the therapeutic efficiency of PDT and PTT. Furthermore, controlling the ROS oxidation and heat reaction to occur within the target sites is crucial to ensuring the therapeutic outcomes [35, 36]. Therefore, it is conceivable that NIR PSs with the ability to target tumor tissues and undergo the type-Ⅰ pathway could offer more efficacious phototherapy. Currently, report of these perfect agents is scarce.
In this work, we have achieved a strong NIR absorption and active tumor targeting through an ingenious design of phthalocyanine molecule by extending the π-conjugate system and incorporating an actively targeted biotin unit. As can be seen from Fig. 1, the synthesized naphthalocyanine (NcPB) exhibits an excellent light absorption with a molar extinction coefficient of 4.9 (×105 L mol−1 cm−1), which is 2.6-fold that of commercial zinc(Ⅱ) phthalocyanine (ZnPc). More importantly, it is also much higher than currently available small molecule organic dyes [37]. Additionally, NcPB has a significant Q-band absorption at 787 nm, which is red-shifted by 119 nm compared to ZnPc. Interestingly, NcPB can be assembled into nanoparticles with different size by adding surfactants in different ratios. It is found that the nanoparticles (NanoNcPB-1) with ultra-small size demonstrated an effective type-Ⅰ photoreaction to produce a large amount of superoxide anion (O2•−) and a notable vibrational relaxation to produce a comparatively efficient photothermal conversion, unlike the traditional PSs based on phthalocyanines that undergo a type-Ⅱ photosensitization. Therefore, this agent combines the action of PDT and PTT, which improves the overall efficacy of phototherapy significantly. Following an intravenous injection, NanoNcPB-1 demonstrated an outstanding tumor accumulation compared to nanoparticles (NanoNcP-1) without biotin-targeting ability. Moreover, NanoNcPB-1 showed a 92% tumor inhibition after combination therapy of PDT and PTT. Excitingly, NanoNcPB-1 can be excreted from the body of mice as raw drugs via the renal clearance pathway. As a result, no obvious histopathological abnormality was observed from normal organs by hematoxylin and eosin (H & E) staining, verifying the excellent biocompatibility of NanoNcPB-1.
We selected the silicon phthalocyanine as the main molecular scaffold for the design of novel PSs because of its following advantages: strong absorption, considerable ROS quantum yield, and adaptable photochemical properties. Here, we adopted a conventional strategy by extending the π-conjugated system to achieve NIR absorption. However, the extension of the π-conjugated system leads to some undesirable defects of this dye, such as large hydrophobic structure, poor water solubility, and severe intermolecular aggregation. To address the side effects of the π-extension strategy, we peripherally modified the naphthalocyanine with a lipophilic long alkyl chain to improve its solubility, as well as axially modified the naphthalocyanine with a polyethylene glycol chain to increase its biocompatibility. On the other hand, due to the abnormal growth and proliferation of cancer cells, the demand for biotin in tumor is higher than that in normal tissues, resulting in the expression of biotin receptors in many cancer cells, which makes it a prospective marker for cancer diagnosis and specific therapy [38]. Ultimately, we modified the biotin unit at the end of the polyethylene glycol to improve its ability to target tumor tissues. The naphthalocyanine (NcPB) with the biotin unit was synthesized through an eleven-step process. The naphthalocyanine without biotin units (NcP) was also synthesized as a control. The detailed synthesis scheme and characterization are shown in Figs. S1–S4 (Supporting information).
Firstly, we used an ultraviolet spectrophotometer to investigate the light absorption capacity of NcPB and NcP in N, N-dimethylformamide (DMF). ZnPc was used as a control because it typically exhibits strong light absorption and photodynamic activity in the optimal phototherapy window. As shown in Fig. 2a, NcPB and NcP exhibit Q-band absorption peaks at 787 nm and 786 nm, respectively. In addition, NcPB and NcP have strong light absorptions, with molar extinction coefficients (ε) of 4.9 and 3.8 (× 105 L mol-1 cm-1), respectively. Light-absorbing capacities of NcPB and NcP are 2.6- and 2.0-fold than that of the commercial ZnPc. According to the previous reports, the energy bandgap (Eg) is frequently used to describe the minimum energy required for a substance to be able to absorb a photon and undergo a jump [39, 40]. Therefore, we investigated the Eg of NcPB and NcP using cyclic voltammetry in order to determine the cause of the increased light absorption. As shown in Fig. 2b and Table S1 (Supporting information), the Eg of ZnPc, NcP and NcPB were calculated to be 1.15, 1.11 and 1.05 eV, respectively. Based on the above results, we speculate that a smaller Eg is favorable for enhanced light absorption. Therefore, compared with ZnPc, NcPB not only achieved a NIR absorption by extending the π-conjugation system, but also enhanced light-absorption capacity by reducing Eg (Fig. 2c). These superior optical properties should open a new avenue for improving the overall efficacy of PDT and PTT.
Next, we took a simple approach to prepare NcPB into a nano agent. The solution of dimethyl sulfoxide (DMSO) involving NcPB was added to phosphate buffered saline (PBS) solutions containing Cremophor EL (CEL) in different volume percentages. As shown in Fig. 3a, in PBS solutions containing 0% CEL, the hydrodynamic diameter of NcPB was 122.6 nm and its polydispersity index (PDI) was 22.4%. A consistent result was also obtained in the transmission electron microscopy (TEM) detection. However, the NcPB in PBS solutions containing CEL contents of 0.1% and 0.5% was observed to be inhomogeneous from the dynamic light scattering (DLS) assay (Fig. S5a in Supporting information). When the content of CEL was increased to 1%, it showed a homogeneous dispersion system of NcPB with a PDI of 15.1% and an ultra-small nano-size of 12.3 nm, which is consistent with TEM imaging results (Fig. 3a). In addition, NcPB in PBS solutions containing 1% CEL showed no significant alteration in particle size over seven days (Fig. S5b in Supporting information). Moreover, we also performed the same method on NcP and used it as a control. The results show that NcP fails to form a nanosystem with uniform particle size in PBS solutions regardless of the adjustment of the CEL content (Fig. S6 in Supporting information). Here, we refer to the nanoparticles formed by assembled NcPB and NcP in PBS solutions without CEL as the NanoNcPB-0 and NanoNcP-0, respectively. Meanwhile, we refer to the nanoparticles formed by assembled NcPB and NcP in PBS solutions containing 1% CEL as the NanoNcPB-1 and NanoNcP-1, respectively.
The above studies show that NanoNcPB-0 and NanoNcPB-1 have different sizes. Herein, we separately studied the absorption spectra of NanoNcPB-0, NanoNcPB-1 in PBS solutions, and NcPB in DMF. As shown in Fig. S7a (Supporting information), NcPB in DMF has a sharp and narrow Q-band absorption peak, while NanoNcPB-0 in PBS has a broad and low Q-band absorption peak. Interestingly, the light absorption capacity of NanoNcPB-1 is weaker than that of NcPB and stronger than that of NanoNcPB-0. In addition, the fluorescence emission intensity of NanoNcPB-1 is also between that of NcPB and NanoNcPB-0 (Fig. S7b in Supporting information). The electronic absorption and fluorescence emission spectra of NanoNcP-1 without the biotin unit are similar to those of NanoNcPB-1. We then investigated the ability of NanoNcPB-0, NanoNcP-0, NanoNcPB-1 and NanoNcP-1 to produce O2•− using the dihydroethidium (DHE) probe. As shown in Fig. 3b and Fig. S8 (Supporting information), both NanoNcPB-1 and NanoNcP-1 were able to produce higher amounts of O2•− and were 10-fold and 8-fold to commercial methylene blue (MB), respectively. However, both NanoNcPB-0 and NanoNcP-0 were less capable of producing O2•− than MB. Therefore, NanoNcPB-1 and NanoNcP-1with ultra-small particle size is more suitable than NanoNcPB-0 and NanoNcP-0 for type-Ⅰ photoreaction. In addition, we also examined the ROS and 1O2 production of NanoNcPB-1 and NanoNcP-1 using the 2, 7-dichlorodi-hydrofluorescein diacetate (DCFH) and singlet oxygen sensor green (SOSG) probes, independently. The results showed that both NanoNcPB-1 and NanoNcP-1 were able to produce more ROS than MB (Fig. 3c and Fig. S9a in Supporting information). However, both of them produce little 1O2 (Fig. 3d and Fig. S9b in Supporting information). These results suggested that NanoNcPB-1 and NanoNcP-1 should exert their photodynamic effects mainly through the type I pathway.
Besides, the photothermal conditions of NanoNcPB-1 and NanoNcP-1 have been investigated. As shown in Fig. 3e, compared to the commercially available photothermal agent indocyanine green (ICG), NanoNcPB-1 has a higher photothermal generation capacity, with temperature rising up to 52.5 ℃ under the 785 nm laser irradiation at a power density of 0.5 W/cm2. In addition, the temperature of NanoNcP-1 is also consistent with that of NanoNcPB-1. Notably, the temperature of ICG showed a tendency to increase and then decrease when under continuous laser irradiation for 10 min, whereas the temperature of NanoNcPB-1 increased and then remained stable. This may be caused by the better photostability of NanoNcPB-1 than ICG. Therefore, the PBS solutions of ICG and NanoNcPB-1 after a continuous laser irradiation for 10 min were subjected to investigate the electronic absorption spectra. As shown in Fig. 3f, the absorbance of ICG decreased significantly after laser irradiation, whereas the absorbance of NanoNcPB-1 remained consistent with that before laser irradiation. This result indicates that NanoNcPB-1 has a better photothermal stability than ICG. Moreover, the photothermal cycling concurrently proved that NanoNcPB-1 can be stabilized in PBS solutions under a sustained laser irradiation for 80 min (Fig. S10a in Supporting information). We then studied the photothermal performances of NanoNcPB-1 at different concentrations and different light power densities. The results showed a more pronounced increase in temperature with increasing concentrations of NanoNcPB-1 (Fig. S10b in Supporting information). When the concentration of NanoNcPB-1 was 40 µmol/L, the temperature could rise to 58.0 ℃ under a laser irradiation at a power density of 0.5 W/cm2. Moreover, the photothermal capacity of NanoNcPB-1 also increased with increasing laser power density. NanoNcPB-1 (20 µmol/L) showed no significant increase in temperature when exposed to a laser power density of 0.1 W/cm2, whereas the temperature could increase up to 52.3 and 63.8 ℃ when exposed to a laser power density of 0.5 and 1.0 W/cm2, respectively (Fig. S10c in Supporting information). These results suggest that NanoNcPB-1 should exerts photodynamic effects under a 785 nm laser irradiation at a power density equal to or less than 0.1 W/cm2, whereas it may perform combined photothermal and photodynamic effects at a power density equal to or greater than 0.5 W/cm2.
According to the aforementioned results, NanoNcPB-1 and NanoNcP-1 show superior photodynamic and photothermal characteristics, which have considerable potential for combination therapy of hypoxic tumor. Next, we proceeded to study the tumor targeting and phototherapy outcome of NanoNcPB-1 by building the subcutaneous hepatocarcinoma (H22) tumor-bearing mice. All animal studies were carried out in compliance with guidelines of the Animal Ethics Committee of Fuzhou University (No. 2023-SG-001), and also approved by the committee. Firstly, the in vivo biodistribution of NanoNcPB-1 was investigated by fluorescence imaging, and NanoNcP-1 was used as a control. As shown in Fig. 4a, at 36 h after intravenous injection, NanoNcPB-1 was significantly enriched in the tumor area. In contrast, NanoNcP-1 was rarely concentrated in the tumor tissues. Consequently, the fluorescence intensity of NanoNcPB-1 was 2.8-fold higher than that of NanoNcP-1 (Fig. 4b). In addition, to further validate that NanoNcPB-1 has the ability to actively select tumor tissues, we also constructed H22 tumor model at different locations in the trunk of ICR mice. The result showed that NanoNcPB-1 intelligently selects H22 tumor regardless of whether the tumor tissues are in the upper or lower trunk of the mouse (Fig. 4a). Secondly, an evaluation of the distribution of NanoNcPB-1 and NanoNcP-1 in the resected tumor and primary organs was conducted by taking the ex vivo fluorescence images at 72 h after intravenous injection. The findings demonstrated that NanoNcPB-1 has a significant fluorescent signal in the resected tumor tissues compared to NanoNcP-1 (Fig. 4c). Additionally, NanoNcPB-1 was taken up more in tumor tissues than in normal tissues, and its fluorescence signal in the tumor tissue was 6.1-fold higher than in liver and 4.7-fold more than in kidney (Fig. 4d).
In addition, we explored the potential of NanoNcPB-1 as a photothermal therapeutic agent and used PBS-injected mice as a control. Tumor tissues were irradiated by 785 nm laser with different power densities for 8 min at 36 h after intravenous injection of NanoNcPB-1, and temperature profiles of the tumor were observed using an infrared thermal imager. As shown in Figs. 4e and f, under laser irradiation at a power density of 0.1 W/cm2, there was no significant temperature trend in both the NanoNcPB-1 and PBS groups. Notably, under the irradiation of a 785 laser with a power density of 0.5 W/cm2, the temperature of the tumor tissues of mice injected with NanoNcPB-1 was significantly increased, rising to 58.0 ℃. However, it was negligible temperature change of PBS-injected mice at a power density of 0.5 W/cm2. Therefore, it is demonstrated that NanoNcPB-1 should perform both photodynamic and photothermal roles under NIR laser illumination at a power density of 0.5 W/cm2.
The remarkable tumor accumulation capacity of NanoNcPB-1 inspired us to investigate its in vivo phototherapeutic efficacy in mice bearing H22 tumor. Six groups of mice (five mice each) were created: (1) handled with PBS in absence of laser irradiation (marked as PBS), (2) handled with PBS combined with 0.5 W/cm2 laser irradiation (marked as PBS + 0.5 W/cm2), (3) handled with PBS combined with 0.1 W/cm2 laser irradiation (marked as PBS + 0.1 W/cm2), (4) handled with NanoNcPB-1 in absence of laser irradiation (marked as NanoNcPB-1), (5) handled with NanoNcPB-1 combined with 0.5 W/cm2 laser irradiation (marked as NanoNcPB-1 + 0.5 W/cm2), and (6) handled with NanoNcPB-1 combined with 0.1 W/cm2 laser irradiation (marked as NanoNcPB-1 + 0.1 W/cm2). Following various treatments, the body weight and tumor volume of each mouse group were assessed every other day. Throughout the course of the treatments, the mice's body weights increased steadily and did not significantly differ between different groups (Fig. 4g). Nonetheless, there was a noticeable difference in the tumor volumes of the mice amongst the various groups. As shown in Fig. 4h, all of the control groups (PBS, PBS + 0.5 W/cm2, PBS + 0.1 W/cm2, and NanoNcPB-1) have a clear level of tumor growth, while the groups of NanoNcPB-1 + 0.1 W/cm2 and NanoNcPB-1 + 0.5 W/cm2 showed a significant inhibition of tumor growth. After 14 days of treatment, NanoNcPB-1 + 0.1 W/cm2-treated mice's tumors showed 65% growth suppression, which should owe to the type-Ⅰ PDT effect. The tumor inhibition of the NanoNcPB-1 + 0.5 W/cm2 group was higher (92%) than that of the NanoNcPB-1 + 0.1 W/cm2 group, indicating that the combination of type-Ⅰ PDT and PTT should be advantageous. The exceptional antitumor efficaciousness of NanoNcPB-1 under laser irradiation was further validated by the representative images (Fig. 4i) and average tumor weights (Fig. 4j). In addition, pathological examination by the H & E staining was performed to evaluate the biological safety of NanoNcPB-1 on primary organs. It indicated that no obvious histopathological abnormality was observed from normal organs, verifying the excellent biocompatibility of NanoNcPB-1 (Fig. S11 in Supporting information).
Finally, we further studied the excretion of NanoNcPB-1 in the mouse body using a small animal in vivo fluorescence imager (Fig. S12 in Supporting information). As shown in Fig. S12a, at 24 h after intravenous injection, supine mice showed a visible fluorescent signal in the bladder, indicating that NanoNcPB-1 can be urinated out of the body. At the same time, we also monitored the fluorescence signals in urine and feces of mice over a period of 72 h (Fig. S12b). It was found that the fluorescence intensity of urine from mice was strongest at 24 h, while the signal at 72 h was negligible (Fig. S12c) in Supporting information, which is consistent with the results of in vivo imaging in supine mice. Moreover, fluorescent signal was also detected in mouse feces, but it was weaker than that in the urine of mice. Meanwhile, the urine is sent for high-resolution mass spectrometry to study the excretion product (Fig. S13 in Supporting information). It was found that the molecular ion peak of naphthalocyanine could be detected in urine. The result indicates that NanoNcPB-1 is excreted from the urine via the kidney in the original molecular manner. Next, we also performed fluorescence imaging of isolated organs and tumor at 24 and 72 h after injection, respectively (Fig. S12d). As shown in Fig. S12e, both the dissected kidney and tumor had significant fluorescent signals at 24 h, whereas there were no signals in the heart, liver, spleen and lung. Notably, the fluorescent signal in the kidney disappeared at 72 h and remained evident only in the tumor tissues. Taken together, NanoNcPB-1 was effectively cleared by the kidneys and subsequently excreted through urine. This efficient clearance mechanism helps prevent the prolonged accumulation of nanoparticles in the body, contributing to the overall safety of their in vivo applications. Eventually, we also performed fluorescence imaging of blood at different time after intravenous injection of NanoNcPB-1 (Fig. S12f). It can undergo rapid in vivo blood circulation, resulting in a strong fluorescent signal at 2 h. However, the fluorescence signal in the blood gradually decreases as time goes on, and it disappears until 72 h (Fig. S12g). This result also provides further evidence that NanoNcPB-1 avoids the prolonged retention in the body of mice. In conclusion, NanoNcPB-1 is a renally removable PS that can be efficiently and rapidly excreted from the body.
In conclusion, a naphthalocyanine PS (NcPB) with an excellent NIR absorption was achieved by a molecular design strategy of extended conjugated units. Meanwhile, NcPB was formed into nanoparticle with an ultra-small size (NanoNcPB-1) through a supramolecular modulation strategy, which exhibited an effective type-Ⅰ photoreaction and thermal generation, making it suitable for phototherapy of hypoxic tumor. As a result, assays reveal that NanoNcPB-1 shows an efficient accumulation to the tumor tissues and has a significant inhibition on solid tumor under the combined treatment of PDT and PTT. More importantly, this agent is rapidly excreted from mice as original medication via the renal clearance pathway, which avoids prolonged retention of PS in the body's circulation. Therefore, this NIR naphthalocyanine PS with outstanding light absorption, active targeting and renal clearance will encourage the continued exploration of clinical agents for phototherapy.
Declaration of competing interest
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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
CRediT authorship contribution statement
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Li Li: Writing – original draft, Resources, Project administration, Methodology. Jiale Wen: Formal analysis. Xiaojun Zhang: Investigation. Shuwen Fu: Methodology. Zixuan Chen: Resources. Kai Huang: Software. Luyue Fang: Supervision. Tinghe Zhao: Validation. Peipei Zhang: Data curation. Xingshu Li: Writing – review & editing, Funding acquisition.
Acknowledgment
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The authors thank the National Natural Science Foundation of China (Nos. T2322004 and 22078066).
Supplementary materials
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Supplementary material associated with this article can be found, in the online version, at doi:10.1016/j.cclet.2024.110290.
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Year 2025 volume 36 Issue 6
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doi: 10.1016/j.cclet.2024.110290
  • Receive Date：2024-04-30
  • Online Date：2025-10-29
  • Published：2025-06-15
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  • Received：2024-04-30
  • Revised：2024-07-21
  • Accepted：2024-07-22
Affiliations
    Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, College of Chemistry, Fuzhou University, Fuzhou 350108, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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