Transcriptomic and metabolomic insights into the roles of exogenous β-hydroxybutyrate acid for the development of rumen epithelium in young goats

Transcriptomic and metabolomic insights into the roles of exogenous β-hydroxybutyrate acid for the development of rumen epithelium in young goats

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Yimin Zhuang^a, Jianmin Chai^a^,^b^,^c, Mahmoud M. Abdelsattar^a^,^d, Yuze Fu^a, Naifeng Zhang^a^,^*

Animal Nutrition | 2023, 15(1) : 10 - 21

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Animal Nutrition | 2023, 15(1): 10-21

• Original Research Article •

Transcriptomic and metabolomic insights into the roles of exogenous β-hydroxybutyrate acid for the development of rumen epithelium in young goats

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Yimin Zhuang^a, Jianmin Chai^a^,^b^,^c, Mahmoud M. Abdelsattar^a^,^d, Yuze Fu^a, Naifeng Zhang^a^,^*

Affiliations

^aKey Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research of Chinese Academy of Agricultural Sciences, Beijing 100081, China

^bDepartment of Animal Science, Division of Agriculture, University of Arkansas, Fayetteville, AR 72701, USA

^cGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan University, Foshan, China

^dDepartment of Animal and Poultry Production, Faculty of Agriculture, South Valley University, 83523 Qena, Egypt

Published: 2023-12-10 doi: 10.1016/j.aninu.2023.02.012

Outline

Abstract

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Beta-hydroxybutyric acid (BHBA), as one of the main metabolic ketones in the rumen epithelium, plays critical roles in cellular growth and metabolism. The ketogenic capacity is associated with the maturation of rumen in young ruminants, and the exogenous BHBA in diet may promote the rumen development. However, the effects of exogenous BHBA on rumen remain unknown. This is the first study to investigate the mechanisms of BHBA on gene expression and metabolism of rumen epithelium using young goats as a model through multi-omics techniques. Thirty-two young goats were divided into control, low dose, middle dose, and high dose groups by supplementation of BHBA in starter (0, 3, 6, and 9g/day, respectively). Results demonstrated the dietary of BHBA promoted the growth performance of young goats and increased width and length of the rumen papilla (P < 0.05). Hub genes in host transcriptome that were positively related to rumen characteristics and BHBA concentration were identified. Several upregulated hub genes including NDUFC1, NDUFB4, NDUFB10, NDUFA11 and NDUFA1 were enriched in the gene ontology (GO) pathway of nicotinamide adenine dinucleotide (NADH) dehydrogenase (ubiquinone) activity, while ATP5ME, ATP5PO and ATP5PF were associated with ATP synthesis. RT-PCR revealed the expression of genes (HMGCS2, BDH1, SLC16A3, etc.) associated with lipolysis increased significantly by BHBA supplementation (P < 0.05). Metabolomics indicated that some metabolites such as glucose, palmitic acid, cortisol and capric acid were also increased (P < 0.05). This study revealed that BHBA promoted rumen development through altering NADH balance and accelerating lipid metabolism, which provides a theoretical guidance for the strategies of gastrointestinal health and development of young ruminants.

Key words

Rumen / Beta-hydroxybutyrate acid / Lipid metabolism / Transcriptome / Metabolomics / Goat

Cite this Article

Yimin Zhuang, Jianmin Chai, Mahmoud M. Abdelsattar, Yuze Fu, Naifeng Zhang. Transcriptomic and metabolomic insights into the roles of exogenous β-hydroxybutyrate acid for the development of rumen epithelium in young goats[J]. Animal Nutrition, 2023 , 15 (1) : 10 -21 . DOI: 10.1016/j.aninu.2023.02.012

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Year 2023 volume 15 Issue 1

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Article Info

doi: 10.1016/j.aninu.2023.02.012

Receive Date：2021-09-06
Online Date：2026-02-03
Published：2023-12-10

Article Data

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History

Received：2021-09-06
Revised：2023-01-29
Accepted：2023-02-17

Affiliations

^aKey Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research of Chinese Academy of Agricultural Sciences, Beijing 100081, China

^bDepartment of Animal Science, Division of Agriculture, University of Arkansas, Fayetteville, AR 72701, USA

^cGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan University, Foshan, China

^dDepartment of Animal and Poultry Production, Faculty of Agriculture, South Valley University, 83523 Qena, Egypt

Corresponding:

^* Corresponding author. E-mail address: zhangnaifeng@caas.cn (N. Zhang).

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表12种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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