Dietary rumen-protected L-arginine or N-carbamylglutamate enhances placental amino acid transport and suppresses angiogenesis and steroid anabolism in underfed pregnant ewes

Dietary rumen-protected L-arginine or N-carbamylglutamate enhances placental amino acid transport and suppresses angiogenesis and steroid anabolism in underfed pregnant ewes

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Hao Zhang^a^,^b, Xia Zha^a^,^b, Bei Zhang^a^,^b, Yi Zheng^a^,^b, Xiaoyun Liu^a^,^b, Mabrouk Elsabagh^c^,^d, Yi Ma^a^,^b, Hongrong Wang^a^,^b, Guihua Shu^a^,^e^,^*, Mengzhi Wang^a^,^b^,^*

Animal Nutrition | 2023, 15(1) : 149 - 158

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Animal Nutrition | 2023, 15(1): 149-158

• Original Research Article •

Dietary rumen-protected L-arginine or N-carbamylglutamate enhances placental amino acid transport and suppresses angiogenesis and steroid anabolism in underfed pregnant ewes

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Hao Zhang^a^,^b, Xia Zha^a^,^b, Bei Zhang^a^,^b, Yi Zheng^a^,^b, Xiaoyun Liu^a^,^b, Mabrouk Elsabagh^c^,^d, Yi Ma^a^,^b, Hongrong Wang^a^,^b, Guihua Shu^a^,^e^,^*, Mengzhi Wang^a^,^b^,^*

Affiliations

^aLaboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

^bJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China

^cDepartment of Animal Production and Technology, Faculty of Agricultural Sciences and Technologies, Niǧde Ömer Halisdemir University, Nigde 51240, Turkey

^dDepartment of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Kafrelsheikh University, KafrelSheikh, Egypt

^eDepartment of Pediatrics, Northern Jiangsu People's Hospital, Clinical Medical College, Yangzhou University, Yangzhou 225001, China

Published: 2023-12-10 doi: 10.1016/j.aninu.2023.08.005

Outline

Abstract

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This study aimed to investigate the effects of dietary supplementation of underfed Hu ewes from d 35 to 110 of gestation with either rumen-protected L-arginine (RP-Arg) or N-carbamylglutamate (NCG) on placental amino acid (AA) transport, angiogenic gene expression, and steroid anabolism. On d 35 of gestation, 32 Hu ewes carrying twin fetuses were randomly divided into four treatment groups, each consisting of eight ewes, and were fed the following diets: A diet providing 100% of NRC's nutrient re-quirements for pregnant ewes (CON); A diet providing 50% of NRC's nutrient requirements for pregnant ewes (RES); RES diet plus 5g/d NCG (RES + NCG); or RES diet plus 20g/d RP-Arg (RES + ARG). On the d 110 of pregnancy, blood samples were taken from the mother, and samples were collected from type A cotyledons (COT; the fetal portions of the placenta). The levels of 17β-estradiol and progesterone in the maternal serum and both the capillary area density (CAD) and capillary surface density (CSD) in type A COT were decreased in response to Arg or NCG supplementation when compared to the RES group. The concentrations of arginine, leucine, putrescine and spermidine in type A COT were higher (P < 0.05) in the RES + ARG or RES + NCG group than in the RES group. The mRNA expression levels of inducible nitric oxide synthase (iNOS) and solute carrier family 15, member 1 (SLC15A1) were increased (P < 0.05) while those of progesterone receptor (PGR) and fibroblast growth factor 2 (FGF2) were decreased in type A COT by supplementation with either NCG or RP-Arg compared to the RES group. The results suggest that providing underfed pregnant ewes from d 35 to 110 of gestation with a diet supplemented with NCG or RP-Arg improves placental AA transport, and reduces the expression of angiogenic growth factor genes and steroid anabolism, leading to better fetal development.

Key words

Angiogenesis factor / L-arginine / N-carbamylglutamate / Placental amino acid transport / Pregnant ewes / Steroid anabolism

Cite this Article

Hao Zhang, Xia Zha, Bei Zhang, Yi Zheng, Xiaoyun Liu, Mabrouk Elsabagh, Yi Ma, Hongrong Wang, Guihua Shu, Mengzhi Wang. Dietary rumen-protected L-arginine or N-carbamylglutamate enhances placental amino acid transport and suppresses angiogenesis and steroid anabolism in underfed pregnant ewes[J]. Animal Nutrition, 2023 , 15 (1) : 149 -158 . DOI: 10.1016/j.aninu.2023.08.005

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Year 2023 volume 15 Issue 1

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Article Info

doi: 10.1016/j.aninu.2023.08.005

Receive Date：2022-03-25
Online Date：2026-02-03
Published：2023-12-10

Article Data

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History

Received：2022-03-25
Revised：2023-07-31
Accepted：2023-08-16

Affiliations

^aLaboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

^bJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China

^cDepartment of Animal Production and Technology, Faculty of Agricultural Sciences and Technologies, Niǧde Ömer Halisdemir University, Nigde 51240, Turkey

^dDepartment of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Kafrelsheikh University, KafrelSheikh, Egypt

^eDepartment of Pediatrics, Northern Jiangsu People's Hospital, Clinical Medical College, Yangzhou University, Yangzhou 225001, China

Corresponding:

^* Corresponding authors.E-mail addresses: yzsbsgh@126.com (G. Shu)

mzwang@yzu.edu.cn (M. Wang).

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表12种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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