Dietary n-3/n-6 polyunsaturated fatty acid ratio modulates growth performance in spotted seabass (<i>Lateolabrax maculatus</i>) through regulating lipid metabolism, hepatic antioxidant capacity and intestinal health

Dietary n-3/n-6 polyunsaturated fatty acid ratio modulates growth performance in spotted seabass (Lateolabrax maculatus) through regulating lipid metabolism, hepatic antioxidant capacity and intestinal health

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Yanzou Dong^a, Yu Wei^a, Ling Wang^a, Kai Song^a, Chunxiao Zhang^a, Kangle Lu^a^,^*, Samad Rahimnejad^b^,^c

Animal Nutrition | 2023, 14(1) : 20 - 31

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Animal Nutrition | 2023, 14(1): 20-31

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Yanzou Dong^a, Yu Wei^a, Ling Wang^a, Kai Song^a, Chunxiao Zhang^a, Kangle Lu^a^,^*, Samad Rahimnejad^b^,^c

Affiliations

^aKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen 361021, China

^bFaculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Vodňany, Zátiší 728, Vodňany 389 25, Czech Republic

^cFish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Murcia 30100, Spain

Published: 2023-09-10 doi: 10.1016/j.aninu.2023.04.005

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An 8-week feeding experiment was carried out to explore the effects of dietary n-3/n-6 polyunsaturated fatty acid (PUFA) ratio on growth performance, lipid metabolism, hepatic antioxidant status, and gut flora of spotted seabass (Lateolabrax maculatus). Six experimental diets were formulated to contain different levels of two purified oil sources including docosahexaenoic and eicosapentaenoic acids enriched oil (n-3) and linoleic acid-enriched oil (n-6) leading to n-3/n-6 PUFA ratios of 0.04, 0.35, 0.66, 1.35, 2.45 and 16.17. Each diet was fed to triplicate groups of juvenile L.maculatus (11.06 ± 0.20g, 30 fish/tank). Final body weight (FBW), weight gain (WG), specific growth rates (SGR), protein efficiency ratio (PER) and feed utilization efficiency increased as n-3/n-6 PUFA ratio increased up to a certain level, and then decreased thereafter. Fish fed the diet with n-3/n-6 PUFA ratio of 0.66 exhibited the highest FBW, WG, SGR and PER and the lowest feed conversion ratio. Lower n-3/n-6 PUFA ratios induced up-regulated expression of lipid synthesis-related genes (fas,acc2 and srebp-1c) and down-regulated expression of lipolysis related genes (atgl, pparα, cpt-1 and aox). Higher expression of lipolysis-related genes (atgl, pparα and cpt-1) was recorded at moderate n-3/n-6 PUFA ratios (0.66 to 1.35). Moreover, inappropriate n-3/n-6 PUFA ratios triggered up-regulation of pro-inflammatory genes (il-6 and tnf-α) and down-regulation of anti-inflammatory genes (il-4 and il-10) in the intestine. The diet with n-3/n-6 PUFA ratio of 0.66 inhibited intestine inflammation, improved intestinal flora richness, increased the abundance of beneficial bacteria such as Lactobacillus, Alloprevotella and Ruminococcus, and reduced the abundance of harmful bacteria including Escherichia-Shigella and Enterococcus. In summary, it could be suggested that a dietary n-3/n-6 PUFA ratio of 0.66 can improve growth performance and feed utilization in L.maculatus, as is deemed to be mediated through regulation of lipid metabolism and intestinal flora.

Key words

Lateolabrax maculatus / Growth performance / Lipid metabolism / Intestinal flora / n-3/n-6 PUFA ratio

Cite this Article

Yanzou Dong, Yu Wei, Ling Wang, Kai Song, Chunxiao Zhang, Kangle Lu, Samad Rahimnejad. Dietary n-3/n-6 polyunsaturated fatty acid ratio modulates growth performance in spotted seabass (Lateolabrax maculatus) through regulating lipid metabolism, hepatic antioxidant capacity and intestinal health[J]. Animal Nutrition, 2023 , 14 (1) : 20 -31 . DOI: 10.1016/j.aninu.2023.04.005

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Year 2023 volume 14 Issue 1

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doi: 10.1016/j.aninu.2023.04.005

Receive Date：2022-08-11
Online Date：2026-02-03
Published：2023-09-10

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History

Received：2022-08-11
Revised：2023-01-12
Accepted：2023-04-04

Affiliations

^aKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen 361021, China

Corresponding:

^* Corresponding author. E-mail address: lukangle@jmu.edu.cn (K. Lu).

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表12种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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