Host gut-derived Bacillus probiotics supplementation improves growth performance, serum and liver immunity, gut health, and resistive capacity against Vibrio harveyi infection in hybrid grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)

Host gut-derived Bacillus probiotics supplementation improves growth performance, serum and liver immunity, gut health, and resistive capacity against Vibrio harveyi infection in hybrid grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)

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Kwaku Amoah^a^,^b^,^c, Beiping Tan^a^,^b^,^c, Shuang Zhang^a^,^b^,^c, Shuyan Chi^a^,^b^,^c, Qihui Yang^a^,^b^,^c, Hongyu Liu^a^,^b^,^c, Yuanzhi Yang^a, Haitao Zhang^c, Xiaohui Dong^a^,^b^,^c^,^*

Animal Nutrition | 2023, 14(1) : 163 - 184

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Animal Nutrition | 2023, 14(1): 163-184

• Original Research Article •

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Kwaku Amoah^a^,^b^,^c, Beiping Tan^a^,^b^,^c, Shuang Zhang^a^,^b^,^c, Shuyan Chi^a^,^b^,^c, Qihui Yang^a^,^b^,^c, Hongyu Liu^a^,^b^,^c, Yuanzhi Yang^a, Haitao Zhang^c, Xiaohui Dong^a^,^b^,^c^,^*

Affiliations

^aLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China

^bAquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, Guangdong 524088, China

^cKey Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong 524000, China

Published: 2023-09-10 doi: 10.1016/j.aninu.2023.05.005

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Abstract

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Several reports have revealed the vital role that probiotics play in fish growth and health. However, few works are available for host gut-derived probiotics on the growth, immunity, and gut microbiota of fish, especially in hybrid grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus) due to their isolation difficulty and functional verification. This study aimed at assessing 3 host gut-derived Bacillus species' effects on the growth, immune and antioxidant-biochemical responses, haematological parameters, intestinal morphology, immune-related gene expression, gut microbiota, and disease resistance against Vibrio harveyi in hybrid grouper. A total of 480 hybrid grouper (initial weight = 9.03 ± 0.02 g) were randomly allotted into 4 groups, namely, the group fed a basal diet without probiotic inclusion (control, B0), the group fed the basal diet with Bacillus velezensis GPSAK4 (BV), the group fed the basal diet with Bacillus subtilis GPSAK9 (BS), and the group fed the basal diet with Bacillus tequilensis GPSAK2 (BT) strains at 1.0 × 10⁹ CFU/g. After a 6-week feeding trial, the results revealed significant improvements (P < 0.05) in the growth performance, whole fish-body proximate composition, blood haematological parameters, serum, liver, and intestinal biochemical indexes, intestinal morphology, and protection against V. harveyi pathogen in the probiotic-treated groups compared with the untreated. Additionally, the expressions of intestinal tight junction genes (occludin and ZO1), pro- and anti-inflammatory genes, including IL1β, IL6, IL8, TNFα, MyD88, IL10, and TGFβ, were upregulated (P < 0.05) after Bacillus species administration. Host gut-derived Bacillus supplementation shaped the gut microbiota by significantly increasing (P < 0.05) the relative abundance of Proteobacteria, Bacteroidetes, Actinobacteria (except the BS group), Acidobacteria (except the BT group), Cyanobacteria (except the BV and BT groups), and Verrucomicrobia phyla, as well as known beneficial genera (Romboutsia, Turicibacter, Epulopiscium, Clostridium_sensu_stricto 1 and 13, Lactobacillus, and Bacillus), but significantly decreased (P < 0.05) the abundance of Firmicutes, Chloroflexi, and Fusobacteria phyla, and purported pathogenic genera (Staphylococcus and Photobacterium) compared with the control group. Collectively, the results suggest that B. velezensis GPSAK4, B. subtilis GPSAK9 (especially this strain), B. tequilensis GPSAK2 dietary supplementation at 1.0 × 10⁹ CFU/g has positive effects on the intestinal health of hybrid grouper via microbial composition modulation, thus enhancing the assimilation and absorption of nutrients to boost fish growth, immunity, and disease resistance.

Key words

Hybrid grouper (♀Epinephelus fuscoguttatus × ♂E. lanceolatus) / Host-associated probiotics / Immune and antioxidant-biochemical / responses / Gut microbiota / Immune-related gene expression / Vibrio harveyi

Cite this Article

Kwaku Amoah, Beiping Tan, Shuang Zhang, Shuyan Chi, Qihui Yang, Hongyu Liu, Yuanzhi Yang, Haitao Zhang, Xiaohui Dong. Host gut-derived Bacillus probiotics supplementation improves growth performance, serum and liver immunity, gut health, and resistive capacity against Vibrio harveyi infection in hybrid grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)[J]. Animal Nutrition, 2023 , 14 (1) : 163 -184 . DOI: 10.1016/j.aninu.2023.05.005

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Year 2023 volume 14 Issue 1

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Article Info

doi: 10.1016/j.aninu.2023.05.005

Receive Date：2022-09-26
Online Date：2026-02-03
Published：2023-09-10

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History

Received：2022-09-26
Revised：2023-05-04
Accepted：2023-05-11

Affiliations

^aLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China

^bAquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, Guangdong 524088, China

^cKey Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong 524000, China

Corresponding:

^* Corresponding author. E-mail address: dongxiaohui2003@163.com (X. Dong).

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表12种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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