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Characterization of a novel bifunctional enzyme from buffalo rumen metagenome and its effect on in vitro ruminal fermentation and microbial community composition
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Zhenxiang Menga, Jing Maa, Zhanying Suna, Chengjian Yangc, Jing Lengd, Weiyun Zhua, Yanfen Chenga, b, *
Animal Nutrition | 2023, 13(1) : 137 - 149
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Animal Nutrition | 2023, 13(1): 137-149
• Original Research Article •
Characterization of a novel bifunctional enzyme from buffalo rumen metagenome and its effect on in vitro ruminal fermentation and microbial community composition
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Zhenxiang Menga, Jing Maa, Zhanying Suna, Chengjian Yangc, Jing Lengd, Weiyun Zhua, Yanfen Chenga, b, *
Affiliations
  • aLaboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095, China
  • bState Key Laboratory of Grassland Agro-Ecosystems, Center for Grassland Microbiome, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730000, China
  • cBuffalo Research Institute, Chinese Academy of Agricultural, Nanning 530000, China
  • dKey Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650000, China
Published: 2023-06-10 doi: 10.1016/j.aninu.2023.01.004
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To efficiently use lignocellulosic materials in ruminants, it is crucial to explore effective enzymes, especially bifunctional enzymes. In this study, a novel stable bifunctional cellulase-xylanase protein from buffalo rumen metagenome was expressed and characterized, CelXyn2. The enzyme displayed optimal activity at pH 6.0 and 45 ℃. The residual endoglucanase and xylanase activities were 90.6% and 86.4% after a 60-min pre-incubation at 55℃. Hydrolysis of rice straw, wheat straw, sheepgrass and sugar beet pulp by CelXyn2 showed its ability to degrade both cellulose and hemicellulose polymers. Treatment with CelXyn2 improved the hydrolysis of agricultural residues with an evident increase in production of total gas, lactate and volatile fatty acids. The results of 16S rRNA and realtime PCR showed that the effect on in vitro ruminal microbial community depended on fermentation substrates. This study demonstrated that CelXyn2 could strengthen lignocellulose hydrolysis and in vitro ruminal fermentation. These characteristics of CelXyn2 distinguish it as a promising candidate for agricultural application.

Bifunctional enzyme  /  In vitro ruminal fermentation  /  Lignocellulose  /  Microbial community
Zhenxiang Meng, Jing Ma, Zhanying Sun, Chengjian Yang, Jing Leng, Weiyun Zhu, Yanfen Cheng. Characterization of a novel bifunctional enzyme from buffalo rumen metagenome and its effect on in vitro ruminal fermentation and microbial community composition[J]. Animal Nutrition, 2023 , 13 (1) : 137 -149 . DOI: 10.1016/j.aninu.2023.01.004
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Year 2023 volume 13 Issue 1
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Article Info
doi: 10.1016/j.aninu.2023.01.004
  • Receive Date：2022-03-29
  • Online Date：2026-02-03
  • Published：2023-06-10
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History
  • Received：2022-03-29
  • Revised：2022-12-26
  • Accepted：2023-01-09
Affiliations
    aLaboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095, China
    bState Key Laboratory of Grassland Agro-Ecosystems, Center for Grassland Microbiome, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730000, China
    cBuffalo Research Institute, Chinese Academy of Agricultural, Nanning 530000, China
    dKey Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650000, China

Corresponding:

* Corresponding author. E-mail address: (Y. Cheng).
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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