Effects of paraprobiotics on bile acid metabolism and liver health in largemouth bass (<i>Micropterus salmoides</i>) fed a cottonseed protein concentrate-based diet

Effects of paraprobiotics on bile acid metabolism and liver health in largemouth bass (Micropterus salmoides) fed a cottonseed protein concentrate-based diet

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Xiaoze Xie^a^,^c, Xiaofang Liang^a, Hao Wang^a, Qiang Zhu^b, Junjun Wang^c, Ying Chang^d, Eric Leclercq^d, Min Xue^a, Jie Wang^a^,^b^,^*

Animal Nutrition | 2023, 13(1) : 302 - 312

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Animal Nutrition | 2023, 13(1): 302-312

• Original Research Article •

Effects of paraprobiotics on bile acid metabolism and liver health in largemouth bass (Micropterus salmoides) fed a cottonseed protein concentrate-based diet

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Xiaoze Xie^a^,^c, Xiaofang Liang^a, Hao Wang^a, Qiang Zhu^b, Junjun Wang^c, Ying Chang^d, Eric Leclercq^d, Min Xue^a, Jie Wang^a^,^b^,^*

Affiliations

^aNational Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China

^bXinjiang Jinlan Plant Protein Co., Ltd, Shihezi 833200, China

^cState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

^dLallemand SAS, Blagnac 31702, France

Published: 2023-06-10 doi: 10.1016/j.aninu.2023.02.011

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Cottonseed protein concentrate is a sustainable fishmeal alternative in aquafeed. A 10-week experiment was conducted to investigate the effects of a cottonseed protein concentrate-based diet with and without multi-strain yeast fractions (MsYF) on growth, bile acid metabolism, and health in largemouth bass. Four hundred fish (54.0 ± 0.0 g) were casually distributed into 16 tanks (4 replicates/diet). Fish were fed with 4 iso-nitrogen and iso-energetic diets 3 times daily, including a fishmeal diet (FM), a soy protein concentrate-based diet (SPC; replacing 81% fishmeal protein), a cottonseed protein concentrate-based diet (CPC; replacing 81% fishmeal protein), and a CPC diet supplemented with 800 mg/kg MsYF (CPCY). Results showed that the survival of SPC was the lowest, i.e., 48%, with no apparent diet effect among other treatments; we omitted the SPC in additional analyses. Fish fed cottonseed protein concentrate-based diets showed lower growth than FM (P < 0.05). Fish fed CPC showed the highest nuclear dense hepatic phenotypes ratio (50%), followed by CPCY (33%) and FM (17%). Further, dietary CPC increased hepatic total cholesterol and triglyceride levels with concurrently increased cholesterol synthesis but decreased triglyceride synthesis-associated transcription levels (P < 0.05). Furthermore, dietary CPC increased bile acid synthesis but decreased bile acid transport-associated transcription levels (P < 0.05), and then induced an increment of plasma cholic acid and hepatic chenodeoxycholic acid content and the decrement of genus Romboustia (P < 0.05). Regarding the effect of MsYF, fish fed CPCY reduced hepatic lipid accumulation and total plasma bile acid content (P < 0.05) compared to CPC, suggesting an improvement in liver health. Also, dietary MsYF could reverse the microbiota community structure showing a similar gut microbial composition to FM. In conclusion, 81% of fishmeal protein replaced by cottonseed protein concentrate suppressed growth and liver health, while dietary MsYF might mitigate the negative impact of a high cottonseed protein concentrate level diet on liver functions via gut microbiota regulation.

Key words

Cottonseed protein concentrate / Multi-yeast strain fractions / Micropterus salmoides / Liver health / Bile acid metabolism / Gut microbiota

Cite this Article

Xiaoze Xie, Xiaofang Liang, Hao Wang, Qiang Zhu, Junjun Wang, Ying Chang, Eric Leclercq, Min Xue, Jie Wang. Effects of paraprobiotics on bile acid metabolism and liver health in largemouth bass (Micropterus salmoides) fed a cottonseed protein concentrate-based diet[J]. Animal Nutrition, 2023 , 13 (1) : 302 -312 . DOI: 10.1016/j.aninu.2023.02.011

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Year 2023 volume 13 Issue 1

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Article Info

doi: 10.1016/j.aninu.2023.02.011

Receive Date：2022-10-23
Online Date：2026-02-03
Published：2023-06-10

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History

Received：2022-10-23
Revised：2023-02-04
Accepted：2023-02-13

Affiliations

^aNational Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China

^bXinjiang Jinlan Plant Protein Co., Ltd, Shihezi 833200, China

^cState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

^dLallemand SAS, Blagnac 31702, France

Corresponding:

^* Corresponding author. E-mail address: wangjie03@caas.cn (J. Wang).

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表12种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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