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Low Ca diet leads to increased Ca retention by changing the gut flora and ileal pH value in laying hens
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Sha Jianga, b, 1, Xinyu Zoua, 1, Miao Maoa, Mi Zhanga, Wenjun Tua, Meilan Jinc, *
Animal Nutrition | 2023, 13(1) : 270 - 281
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Animal Nutrition | 2023, 13(1): 270-281
• Original Research Article •
Low Ca diet leads to increased Ca retention by changing the gut flora and ileal pH value in laying hens
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Sha Jianga, b, 1, Xinyu Zoua, 1, Miao Maoa, Mi Zhanga, Wenjun Tua, Meilan Jinc, *
Affiliations
  • aJoint International Research Laboratory of Animal Health and Animal Food Safety, College of Veterinary Medicine, Southwest University, Chongqing, 400715, China
  • bImmunology Research Center, Medical Research Institute, Southwest University, Chongqing, 402460, China
  • cLaboratory Animal Center, Southwest University, Chongqing, 400715, China
Published: 2023-06-10 doi: 10.1016/j.aninu.2023.02.006
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Osteoporosis is a common degenerative metabolic bone disease in caged laying hens. Intensive egg production mobilizing large amounts of Ca from bone for eggshell formation, consequently leading to Ca deficiency, has been recognized as a critical factor causing osteoporosis in commercial laying hens. The aim of this study was to examine the effect of Ca deficiency on the function of the gut microbiota-bone axis and related egg production traits and bone health in laying hens. Twenty-four 48-week-old laying hens were fed a control diet (Control, 3.72%) or a low Ca diet (LC, 2.04%) for 60 d (n = 12). Compared to the Control hens, the LC hens had higher levels of alkaline phosphatase and tartrate resistant acid phosphatase (P < 0.05) with lower bone strength, eggshell thickness, and eggshell strength (P < 0.05). In addition, the LC hens had higher plasma estradiol concentrations, while having lower concentrations of interleukin-1 (IL-1) and IL-6. The LC hens also had a lower pH value in the ileum with an increased Ca retention. The principal co-ordinates analysis showed significantly separate cecal microbiota populations between the Control and LC hens. The Prevotellaceae_UCG-001, Subdoligranulum, Peptococcus, and Eubacterium_hallii_group (P < 0.05) were higher, while the CHKC1001 and Sutterella (P < 0.05) were lower at the genus level in the LC hens. In addition, Prevotellaceae_UCG-001, Subdoligranulum and Eubacterium_hallii_group had a negative correlation, while Sutterella was positively correlated with ileal pH values. The transcriptome analysis revealed that the low Ca diet caused 20 and 31 genes to be significantly up-and down-regulated, respectively. The gene expressions of cystic fibrosis transmembrane conductance regulator, solute carrier family 26 member 3 of the anion exchangers, and mitogenactivated protein kinase 12 of pro-inflammatory factors were lower in the LC birds, which was correlated with the lower ileal pH values. These results suggest that the hens with low Ca diet-induced osteoporosis have an increased intestinal Ca retention with a decreased ileal pH value, correlated with the changes in Prevotellaceae_UCG-001, Subdoligranulum, and Eubacterium_hallii_group of beneficial genera. The results provide insights for further understanding and preventing osteoporosis in laying hens.

Low Ca diet  /  Ca retention  /  Microbiota  /  Transcriptional profiling  /  Hen
Sha Jiang, Xinyu Zou, Miao Mao, Mi Zhang, Wenjun Tu, Meilan Jin. Low Ca diet leads to increased Ca retention by changing the gut flora and ileal pH value in laying hens[J]. Animal Nutrition, 2023 , 13 (1) : 270 -281 . DOI: 10.1016/j.aninu.2023.02.006
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Year 2023 volume 13 Issue 1
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Article Info
doi: 10.1016/j.aninu.2023.02.006
  • Receive Date：2022-04-07
  • Online Date：2026-02-03
  • Published：2023-06-10
Article Data
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History
  • Received：2022-04-07
  • Revised：2023-01-16
  • Accepted：2023-02-09
Affiliations
    aJoint International Research Laboratory of Animal Health and Animal Food Safety, College of Veterinary Medicine, Southwest University, Chongqing, 400715, China
    bImmunology Research Center, Medical Research Institute, Southwest University, Chongqing, 402460, China
    cLaboratory Animal Center, Southwest University, Chongqing, 400715, China

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* Corresponding author. E-mail address: (M. Jin).
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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