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Sodium acetate promotes fat synthesis by suppressing TATA element modulatory factor 1 in bovine mammary epithelial cells
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Chaochao Luob, c, Nan Lia, *, Qingzhu Wange, Chunjiang Lia, d, *
Animal Nutrition | 2023, 13(1) : 126 - 136
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Animal Nutrition | 2023, 13(1): 126-136
• Original Research Article •
Sodium acetate promotes fat synthesis by suppressing TATA element modulatory factor 1 in bovine mammary epithelial cells
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Chaochao Luob, c, Nan Lia, *, Qingzhu Wange, Chunjiang Lia, d, *
Affiliations
  • aSchool of Basic Medical Sciences, Jiamusi University, Jiamusi, Heilongjiang, 154002, China
  • bCollege of Life Sciences, Shihezi University, Shihezi, Xinjiang, 832003, China
  • cDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, 510515, China
  • dMudanjiang Normal University, Mudanjiang, Heilongjiang, 157011, China
  • eHarbin Weike Biotechnology Co., Ltd, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, 150069, China
Published: 2023-06-10 doi: 10.1016/j.aninu.2023.01.002
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Short-chain fatty acids are important nutrients that regulate milk fat synthesis. They regulate milk synthesis via the sterol regulatory element binding protein 1 (SREBP1) pathway; however, the details are still unknown. Here, the regulation and mechanism of sodium acetate (SA) in milk fat synthesis in bovine mammary epithelial cells (BMECs) were assessed. BMECs were treated with SA supplementation (SA+) or without SA supplementation (SA-), and milk fat synthesis and activation of the SREBP1 pathway were increased (P = 0.0045; P = 0.0042) by SA+ and decreased (P = 0.0068; P = 0.0031) by SA-, respectively. Overexpression or inhibition of SREBP1 demonstrated that SA promoted milk fat synthesis (P = 0.0045) via the SREBP1 pathway. Overexpression or inhibition of TATA element modulatory factor 1 (TMF1) demonstrated that TMF1 suppressed activation of the SREBP1 pathway (P = 0.0001) and milk fat synthesis (P = 0.0022) activated by SA+. Overexpression or inhibition of TMF1 and SREBP1 showed that TMF1 suppressed milk fat synthesis (P = 0.0073) through the SREBP1 pathway. Coimmunoprecipitation analysis revealed that TMF1 interacted with SREBP1 in the cytoplasm and suppressed the nuclear localization of SREBP1 (P = 0.0066). The absence or presence of SA demonstrated that SA inhibited the expression of TMF1 (P = 0.0002) and the interaction between TMF1 and SREBP1 (P = 0.0001). Collectively, our research suggested that TMF1 was a new negative regulator of milk fat synthesis. In BMECs, SA promoted the SREBP1 pathway and milk fat synthesis by suppressing TMF1. This study enhances the current understanding of the regulation of milk fat synthesis and provides new scientific data for the regulation of milk fat synthesis.

Sodium acetate  /  Milk fat synthesis  /  Sterol regulatory element-binding protein 1  /  TATA element modulatory factor 1  /  Bovine mammary epithelial cell
Chaochao Luo, Nan Li, Qingzhu Wang, Chunjiang Li. Sodium acetate promotes fat synthesis by suppressing TATA element modulatory factor 1 in bovine mammary epithelial cells[J]. Animal Nutrition, 2023 , 13 (1) : 126 -136 . DOI: 10.1016/j.aninu.2023.01.002
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Year 2023 volume 13 Issue 1
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Article Info
doi: 10.1016/j.aninu.2023.01.002
  • Receive Date：2021-08-26
  • Online Date：2026-02-03
  • Published：2023-06-10
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History
  • Received：2021-08-26
  • Revised：2023-01-03
  • Accepted：2023-01-06
Affiliations
    aSchool of Basic Medical Sciences, Jiamusi University, Jiamusi, Heilongjiang, 154002, China
    bCollege of Life Sciences, Shihezi University, Shihezi, Xinjiang, 832003, China
    cDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, 510515, China
    dMudanjiang Normal University, Mudanjiang, Heilongjiang, 157011, China
    eHarbin Weike Biotechnology Co., Ltd, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, 150069, China

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* Corresponding authors. E-mail addresses: (N. Li)
(C. Li).
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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