Proteomic and phosphoproteomic analysis reveal threonine deficiency increases hepatic lipid deposition in Pekin ducks via reducing STAT phosphorylation

Proteomic and phosphoproteomic analysis reveal threonine deficiency increases hepatic lipid deposition in Pekin ducks via reducing STAT phosphorylation

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Yong Jiang^a^,^c, Zhong Zhuang^a, Wenqia Jia^a, Zhiguo Wen^d^,^*, Ming Xie^b, Hao Bai^a, Yulin Bi^a, Zhixiu Wang^a, Guobin Chang^a, Shuisheng Hou^b, Guohong Chen^a^,^*

Animal Nutrition | 2023, 13(1) : 249 - 260

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Animal Nutrition | 2023, 13(1): 249-260

• Original Research Article •

Proteomic and phosphoproteomic analysis reveal threonine deficiency increases hepatic lipid deposition in Pekin ducks via reducing STAT phosphorylation

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Yong Jiang^a^,^c, Zhong Zhuang^a, Wenqia Jia^a, Zhiguo Wen^d^,^*, Ming Xie^b, Hao Bai^a, Yulin Bi^a, Zhixiu Wang^a, Guobin Chang^a, Shuisheng Hou^b, Guohong Chen^a^,^*

Affiliations

^aCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

^bState Key Laboratory of Animal Nutrition, Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

^cJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China

^dKey Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Published: 2023-06-10 doi: 10.1016/j.aninu.2023.01.008

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Abstract

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Dietary threonine (Thr) deficiency enhances triglyceride (TG) deposition in the liver of Pekin ducks, which injures hepatic function and impairs growth performance. However, the underlying molecular mechanisms remain unclear. In the present study, we investigated the effects of dietary Thr deficiency on the expressions of proteins and phosphoproteins in liver of Pekin ducks, to identify the underlying molecular changes. A total of 300 one-day-old ducklings were divided into 3 groups with 10 replicates of 10 birds. All ducks were fed corn-wheat-peanut meal diets containing 0.46%, 0.71%, and 0.96% Thr, respectively, from 1 to 21 days of age. Growth performance, serum parameters, hepatic TG content, and expression of genes involved in lipid metabolism of Pekin ducks were determined. A Thr deficiency group (Thr-D, 0.46% Thr) and a Thr sufficiency group (Thr-S, 0.71% Thr) were selected for subsequent proteomic and phosphoproteomic analysis. The results showed that Thr-D reduced the growth performance (P < 0.001), and increased the plasma concentrations of cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and hepatic TG (P < 0.05). Thr-D increased gene expression related to fatty acid and TG synthesis (P < 0.05). A total of 176 proteins and 259 phosphosites (containing 198 phosphoproteins) were observed to be differentially expressed as a result of Thr-D. The upregulated proteins were enriched in the pathway related to amino acid metabolism, peroxisome. The downregulated proteins were enriched in linolenic and arachidonic acid metabolism, and the Janus kinasesignal transducer and activator of transcription (JAK-STAT) signaling pathway. The upregulated phosphoproteins were enriched in the pathways related to fatty acid biosynthesis, fructose and mannose metabolism, and glycolysis/gluconeogenesis. Thr-D reduced the phosphorylation of STAT1 at S729 and STAT3 at S728, and expression of STAT5B. In contrast, Thr-D increased non-receptor tyrosine-protein kinase (TYK2) expression and STAT1 phosphorylation at S649. Taken together, dietary Thr-D increased hepatic TG accumulation by upregulating the expression of genes and proteins, and phosphoproteins related to fatty acid and triglyceride synthesis. Furthermore, these processes might be regulated by the JAK-STAT signaling pathway, especially the phosphorylation of STAT1 and STAT3.

Key words

Threonine deficiency / Triglyceride accumulation / Proteomic / Phosphoproteomic / Pekin duck

Cite this Article

Yong Jiang, Zhong Zhuang, Wenqia Jia, Zhiguo Wen, Ming Xie, Hao Bai, Yulin Bi, Zhixiu Wang, Guobin Chang, Shuisheng Hou, Guohong Chen. Proteomic and phosphoproteomic analysis reveal threonine deficiency increases hepatic lipid deposition in Pekin ducks via reducing STAT phosphorylation[J]. Animal Nutrition, 2023 , 13 (1) : 249 -260 . DOI: 10.1016/j.aninu.2023.01.008

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Year 2023 volume 13 Issue 1

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Article Info

doi: 10.1016/j.aninu.2023.01.008

Receive Date：2022-07-16
Online Date：2026-02-03
Published：2023-06-10

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History

Received：2022-07-16
Revised：2023-01-16
Accepted：2023-01-19

Affiliations

^aCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

^dKey Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Corresponding:

^* Corresponding authors. E-mail addresses: wenzhiguo@caas.cn (Z. Wen)

ghchen2019@yzu.edu.cn (G. Chen).

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表12种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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