Zearalenone induces apoptosis and autophagy by regulating endoplasmic reticulum stress signalling in porcine trophectoderm cells

Zearalenone induces apoptosis and autophagy by regulating endoplasmic reticulum stress signalling in porcine trophectoderm cells

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Jun Bai^a, Jun Li^a, Ning Liu^a, Hai Jia^a, Xuemeng Si^a, Yusong Zhou^a, Zhian Zhai^a, Ying Yang^a, Fazheng Ren^b, Zhenlong Wu^a^,^b^,^*

Animal Nutrition | 2023, 12(1) : 186 - 199

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Animal Nutrition | 2023, 12(1): 186-199

• Original Research Article •

Zearalenone induces apoptosis and autophagy by regulating endoplasmic reticulum stress signalling in porcine trophectoderm cells

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Jun Bai^a, Jun Li^a, Ning Liu^a, Hai Jia^a, Xuemeng Si^a, Yusong Zhou^a, Zhian Zhai^a, Ying Yang^a, Fazheng Ren^b, Zhenlong Wu^a^,^b^,^*

Affiliations

^aState Key Laboratory of Animal Nutrition, Department of Companion Animal Science, China Agricultural University, Beijing, 100193, China

^bBeijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, 100193, China

Published: 2023-03-10 doi: 10.1016/j.aninu.2022.08.016

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Abstract

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Zearalenone (ZEA), a mycotoxin produced mainly by fungi belonging to Fusarium species in foods and feeds, causes a serious hazard to humans and animals. Numerous studies have revealed that ingesting ZEA can disrupt the reproductive function and impair the reproductive process in animals. This experiment was to investigate the toxicological effect and the mechanism of ZEA exposure on reproduction in pigs during early stages of pregnancy. In the present study, we treated with 0 to 80 μmol/L ZEA for 12 or 24 h in trophoblast ectoderm (pTr) cells. The results showed that ZEA had significantly decreased cell proliferation (P < 0.05), which was accompanied by DNA damage-related cell cycle arrest at G2/M phase, activation of the apoptosis and endoplasmic reticulum (ER) stress, as well as impairment of barrier function (P < 0.05). Western blot analysis and transmission electron microscopy (TEM) showed that exposure to ZEA can activation of autophagy in pTr cells. Importantly, pretreatment with chloroquine (CQ) or 3-methyladenine (3-MA) led to increased apoptosis in pTr cells. Interestingly, pTr cells pretreated with 4-phenylbutyric acid (4-PBA), an inhibitor of ER stress, resulted in reduced cell death in pTr cells, indicating a critical role for ER stress in the activation of autophagy. In conclusion, these results reveal that ZEA-triggered ER stress is critical for the cell fate decision of pTr cells during early porcine embryonic development. Application of small molecules with ability of blocking ER stress might be ther apeutic option to reduce the deleterious effect of ZEA in pregnant animals.

Key words

Zearalenone / Porcine trophectoderm / Apoptosis / Endoplasmic reticulum stress / Autophagy

Cite this Article

Jun Bai, Jun Li, Ning Liu, Hai Jia, Xuemeng Si, Yusong Zhou, Zhian Zhai, Ying Yang, Fazheng Ren, Zhenlong Wu. Zearalenone induces apoptosis and autophagy by regulating endoplasmic reticulum stress signalling in porcine trophectoderm cells[J]. Animal Nutrition, 2023 , 12 (1) : 186 -199 . DOI: 10.1016/j.aninu.2022.08.016

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Year 2023 volume 12 Issue 1

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Article Info

doi: 10.1016/j.aninu.2022.08.016

Receive Date：2022-01-23
Online Date：2026-02-03
Published：2023-03-10

Article Data

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History

Received：2022-01-23
Revised：2022-08-02
Accepted：2022-08-24

Affiliations

^aState Key Laboratory of Animal Nutrition, Department of Companion Animal Science, China Agricultural University, Beijing, 100193, China

^bBeijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, 100193, China

Corresponding:

^* Corresponding author. E-mail address: wuzhenlong@cau.edu.cn (Z. Wu).

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表12种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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